Strain Fitness in Rhodanobacter sp. FW510-T8 around OKGIIK_11435

Experiment: R2A_PIPES with Nickel (II) chloride 424 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntribD and nrdR are separated by 95 nucleotidesnrdR and glyA are separated by 96 nucleotides OKGIIK_11430: ribD - bifunctional diaminohydroxyphosphoribosylaminopyrimidine deaminase/5-amino-6-(5-phosphoribosylamino)uracil reductase RibD, at 2,454,344 to 2,455,462 ribD OKGIIK_11435: nrdR - transcriptional regulator NrdR, at 2,455,558 to 2,456,070 nrdR OKGIIK_11440: glyA - serine hydroxymethyltransferase, at 2,456,167 to 2,457,435 glyA Position (kb) 2455 2456 2457Strain fitness (log2 ratio) -2 -1 0 1 2at 2455.450 kb on - strandat 2455.479 kb on - strandat 2455.479 kb on - strandat 2455.479 kb on - strandat 2455.479 kb on - strandat 2455.479 kb on - strandat 2455.479 kb on - strandat 2455.545 kb on + strandat 2455.545 kb on + strandat 2455.545 kb on + strandat 2455.545 kb on + strandat 2455.545 kb on + strandat 2455.679 kb on + strand, within nrdRat 2455.679 kb on + strand, within nrdRat 2455.680 kb on - strand, within nrdRat 2455.700 kb on + strand, within nrdRat 2455.701 kb on - strand, within nrdRat 2455.701 kb on - strand, within nrdRat 2455.701 kb on - strand, within nrdRat 2455.701 kb on - strand, within nrdRat 2455.701 kb on - strand, within nrdRat 2455.808 kb on - strand, within nrdRat 2455.808 kb on - strand, within nrdRat 2456.071 kb on - strandat 2456.071 kb on - strandat 2456.071 kb on - strandat 2456.071 kb on - strandat 2456.088 kb on + strandat 2456.089 kb on - strandat 2456.089 kb on - strandat 2456.174 kb on + strandat 2456.175 kb on - strandat 2456.300 kb on + strand, within glyAat 2456.300 kb on + strand, within glyAat 2456.300 kb on + strand, within glyAat 2456.301 kb on - strand, within glyAat 2456.301 kb on - strand, within glyAat 2456.301 kb on - strand, within glyAat 2456.301 kb on - strand, within glyAat 2456.310 kb on + strand, within glyAat 2456.561 kb on + strand, within glyAat 2456.561 kb on + strand, within glyAat 2456.561 kb on + strand, within glyAat 2456.561 kb on + strand, within glyAat 2456.561 kb on + strand, within glyAat 2456.562 kb on - strand, within glyAat 2456.562 kb on - strand, within glyAat 2456.562 kb on - strand, within glyAat 2456.562 kb on - strand, within glyAat 2456.849 kb on + strand, within glyAat 2456.849 kb on + strand, within glyAat 2456.850 kb on - strand, within glyAat 2456.850 kb on - strand, within glyAat 2456.850 kb on - strand, within glyAat 2456.850 kb on - strand, within glyAat 2456.906 kb on + strand, within glyAat 2456.907 kb on - strand, within glyAat 2456.972 kb on + strand, within glyAat 2456.973 kb on - strand, within glyAat 2457.002 kb on + strand, within glyAat 2457.002 kb on + strand, within glyAat 2457.002 kb on + strand, within glyAat 2457.003 kb on - strand, within glyAat 2457.003 kb on - strand, within glyA

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM
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2,455,450 - -1.6
2,455,479 - -2.2
2,455,479 - -0.5
2,455,479 - +0.8
2,455,479 - +1.8
2,455,479 - -0.7
2,455,479 - -0.9
2,455,545 + -1.0
2,455,545 + -0.3
2,455,545 + -1.0
2,455,545 + +0.6
2,455,545 + +1.2
2,455,679 + nrdR OKGIIK_11435 0.24 -1.0
2,455,679 + nrdR OKGIIK_11435 0.24 -1.0
2,455,680 - nrdR OKGIIK_11435 0.24 -0.0
2,455,700 + nrdR OKGIIK_11435 0.28 +0.6
2,455,701 - nrdR OKGIIK_11435 0.28 -0.3
2,455,701 - nrdR OKGIIK_11435 0.28 -0.4
2,455,701 - nrdR OKGIIK_11435 0.28 -1.6
2,455,701 - nrdR OKGIIK_11435 0.28 -1.2
2,455,701 - nrdR OKGIIK_11435 0.28 -0.8
2,455,808 - nrdR OKGIIK_11435 0.49 +0.4
2,455,808 - nrdR OKGIIK_11435 0.49 -1.6
2,456,071 - -0.4
2,456,071 - -0.0
2,456,071 - +0.7
2,456,071 - -0.7
2,456,088 + +0.2
2,456,089 - +0.0
2,456,089 - +0.6
2,456,174 + +1.9
2,456,175 - -1.6
2,456,300 + glyA OKGIIK_11440 0.10 +1.1
2,456,300 + glyA OKGIIK_11440 0.10 -0.2
2,456,300 + glyA OKGIIK_11440 0.10 -1.8
2,456,301 - glyA OKGIIK_11440 0.11 -1.8
2,456,301 - glyA OKGIIK_11440 0.11 +0.6
2,456,301 - glyA OKGIIK_11440 0.11 -0.4
2,456,301 - glyA OKGIIK_11440 0.11 -0.6
2,456,310 + glyA OKGIIK_11440 0.11 -1.0
2,456,561 + glyA OKGIIK_11440 0.31 +0.6
2,456,561 + glyA OKGIIK_11440 0.31 -2.0
2,456,561 + glyA OKGIIK_11440 0.31 -1.4
2,456,561 + glyA OKGIIK_11440 0.31 -0.6
2,456,561 + glyA OKGIIK_11440 0.31 -2.3
2,456,562 - glyA OKGIIK_11440 0.31 -1.8
2,456,562 - glyA OKGIIK_11440 0.31 -1.6
2,456,562 - glyA OKGIIK_11440 0.31 -1.2
2,456,562 - glyA OKGIIK_11440 0.31 +1.3
2,456,849 + glyA OKGIIK_11440 0.54 -0.8
2,456,849 + glyA OKGIIK_11440 0.54 -1.0
2,456,850 - glyA OKGIIK_11440 0.54 -1.0
2,456,850 - glyA OKGIIK_11440 0.54 -0.9
2,456,850 - glyA OKGIIK_11440 0.54 -1.2
2,456,850 - glyA OKGIIK_11440 0.54 -1.6
2,456,906 + glyA OKGIIK_11440 0.58 -0.6
2,456,907 - glyA OKGIIK_11440 0.58 -1.4
2,456,972 + glyA OKGIIK_11440 0.63 -2.6
2,456,973 - glyA OKGIIK_11440 0.64 -0.6
2,457,002 + glyA OKGIIK_11440 0.66 -0.0
2,457,002 + glyA OKGIIK_11440 0.66 -1.4
2,457,002 + glyA OKGIIK_11440 0.66 +0.6
2,457,003 - glyA OKGIIK_11440 0.66 -0.8
2,457,003 - glyA OKGIIK_11440 0.66 -1.6

Or see this region's nucleotide sequence