Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_11055 and cheW are separated by 128 nucleotides cheW and ompR overlap by 4 nucleotides ompR and pilG are separated by 22 nucleotides pilG and gshB are separated by 345 nucleotides
OKGIIK_11055: OKGIIK_11055 - chemotaxis protein, at 2,371,190 to 2,373,220
_11055
OKGIIK_11060: cheW - chemotaxis protein CheW, at 2,373,349 to 2,373,888
cheW
OKGIIK_11065: ompR - two-component system response regulator, at 2,373,885 to 2,374,256
ompR
OKGIIK_11070: pilG - twitching motility response regulator PilG, at 2,374,279 to 2,374,677
pilG
OKGIIK_11075: gshB - glutathione synthase, at 2,375,023 to 2,376,000
gshB
Position (kb)
2373
2374
2375 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2372.916 kb on + strand, within OKGIIK_11055 at 2372.916 kb on + strand, within OKGIIK_11055 at 2372.916 kb on + strand, within OKGIIK_11055 at 2372.916 kb on + strand, within OKGIIK_11055 at 2372.917 kb on - strand, within OKGIIK_11055 at 2373.155 kb on + strand at 2373.156 kb on - strand at 2373.156 kb on - strand at 2373.176 kb on + strand at 2373.176 kb on + strand at 2373.176 kb on + strand at 2373.176 kb on + strand at 2373.176 kb on + strand at 2373.176 kb on + strand at 2373.177 kb on - strand at 2373.177 kb on - strand at 2373.177 kb on - strand at 2373.177 kb on - strand at 2373.177 kb on - strand at 2373.177 kb on - strand at 2373.183 kb on + strand at 2373.184 kb on - strand at 2373.211 kb on + strand at 2373.211 kb on + strand at 2373.211 kb on + strand at 2373.211 kb on + strand at 2373.211 kb on + strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.212 kb on - strand at 2373.237 kb on + strand at 2373.237 kb on + strand at 2373.237 kb on + strand at 2373.238 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.246 kb on - strand at 2373.574 kb on - strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.584 kb on + strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.585 kb on - strand, within cheW at 2373.842 kb on + strand at 2373.842 kb on + strand at 2373.842 kb on + strand at 2373.842 kb on + strand at 2373.842 kb on + strand at 2373.842 kb on + strand at 2373.843 kb on - strand at 2373.843 kb on - strand at 2373.843 kb on - strand at 2373.843 kb on - strand at 2374.061 kb on + strand, within ompR at 2374.164 kb on + strand, within ompR at 2374.164 kb on + strand, within ompR at 2374.164 kb on + strand, within ompR at 2374.165 kb on - strand, within ompR at 2374.165 kb on - strand, within ompR at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.247 kb on + strand at 2374.248 kb on - strand at 2374.248 kb on - strand at 2374.248 kb on - strand at 2374.248 kb on - strand at 2374.248 kb on - strand at 2374.248 kb on - strand at 2374.355 kb on + strand, within pilG at 2374.355 kb on + strand, within pilG at 2374.355 kb on + strand, within pilG at 2374.355 kb on + strand, within pilG at 2374.355 kb on + strand, within pilG at 2374.355 kb on + strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.356 kb on - strand, within pilG at 2374.476 kb on - strand, within pilG at 2374.573 kb on + strand, within pilG at 2374.573 kb on + strand, within pilG at 2374.573 kb on + strand, within pilG at 2374.573 kb on + strand, within pilG at 2374.573 kb on + strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.574 kb on - strand, within pilG at 2374.668 kb on + strand at 2374.668 kb on + strand at 2374.668 kb on + strand at 2374.668 kb on + strand at 2374.669 kb on - strand at 2374.669 kb on - strand at 2374.669 kb on - strand at 2374.669 kb on - strand at 2374.669 kb on - strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.691 kb on + strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.692 kb on - strand at 2374.723 kb on + strand at 2374.723 kb on + strand at 2374.723 kb on + strand at 2374.723 kb on + strand at 2374.724 kb on - strand at 2374.793 kb on + strand at 2374.793 kb on + strand at 2374.794 kb on - strand at 2374.807 kb on - strand at 2374.834 kb on - strand at 2374.834 kb on - strand at 2374.834 kb on - strand at 2374.834 kb on - strand at 2374.863 kb on + strand at 2374.863 kb on + strand at 2374.863 kb on + strand at 2374.863 kb on + strand at 2374.863 kb on + strand at 2374.863 kb on + strand at 2374.864 kb on - strand at 2374.864 kb on - strand at 2374.864 kb on - strand at 2374.864 kb on - strand at 2374.864 kb on - strand at 2374.903 kb on + strand at 2374.903 kb on + strand at 2374.903 kb on + strand at 2374.903 kb on + strand at 2374.903 kb on + strand at 2374.903 kb on + strand at 2374.903 kb on + strand at 2374.904 kb on - strand at 2374.904 kb on - strand at 2374.904 kb on - strand at 2374.904 kb on - strand at 2374.904 kb on - strand at 2374.963 kb on + strand at 2374.963 kb on + strand at 2374.963 kb on + strand at 2374.963 kb on + strand at 2374.963 kb on + strand at 2374.963 kb on + strand at 2374.964 kb on - strand at 2374.964 kb on - strand at 2374.964 kb on - strand at 2374.964 kb on - strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.966 kb on + strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.967 kb on - strand at 2374.968 kb on + strand at 2374.968 kb on + strand at 2374.968 kb on + strand at 2374.968 kb on + strand at 2374.968 kb on + strand at 2374.969 kb on - strand at 2374.969 kb on - strand at 2374.969 kb on - strand at 2374.969 kb on - strand at 2375.072 kb on + strand at 2375.073 kb on - strand at 2375.073 kb on - strand at 2375.134 kb on + strand, within gshB at 2375.134 kb on + strand, within gshB at 2375.135 kb on - strand, within gshB at 2375.135 kb on - strand, within gshB at 2375.135 kb on - strand, within gshB at 2375.135 kb on - strand, within gshB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,372,916 + OKGIIK_11055 0.85 -2.3 2,372,916 + OKGIIK_11055 0.85 -1.3 2,372,916 + OKGIIK_11055 0.85 -1.1 2,372,916 + OKGIIK_11055 0.85 -1.0 2,372,917 - OKGIIK_11055 0.85 -1.5 2,373,155 + +0.7 2,373,156 - -0.8 2,373,156 - +0.4 2,373,176 + -0.8 2,373,176 + -1.8 2,373,176 + -0.3 2,373,176 + -0.4 2,373,176 + -3.1 2,373,176 + -0.3 2,373,177 - -1.4 2,373,177 - -2.3 2,373,177 - -1.5 2,373,177 - -0.4 2,373,177 - -2.3 2,373,177 - -1.1 2,373,183 + -0.0 2,373,184 - +0.2 2,373,211 + +0.4 2,373,211 + -0.5 2,373,211 + -0.2 2,373,211 + +0.6 2,373,211 + -1.4 2,373,212 - +0.4 2,373,212 - +0.4 2,373,212 - -1.0 2,373,212 - -0.7 2,373,212 - -1.4 2,373,212 - -1.2 2,373,212 - +1.1 2,373,212 - -1.6 2,373,212 - -1.6 2,373,212 - -0.4 2,373,237 + +0.4 2,373,237 + -1.1 2,373,237 + -0.3 2,373,238 - +1.3 2,373,246 - -1.8 2,373,246 - -0.0 2,373,246 - -1.0 2,373,246 - -0.5 2,373,246 - -1.9 2,373,246 - -0.8 2,373,246 - -0.0 2,373,246 - -0.9 2,373,246 - -0.0 2,373,246 - -0.9 2,373,246 - -0.2 2,373,574 - cheW OKGIIK_11060 0.42 +1.7 2,373,584 + cheW OKGIIK_11060 0.44 -0.8 2,373,584 + cheW OKGIIK_11060 0.44 -0.8 2,373,584 + cheW OKGIIK_11060 0.44 +1.1 2,373,584 + cheW OKGIIK_11060 0.44 -0.3 2,373,584 + cheW OKGIIK_11060 0.44 +0.2 2,373,584 + cheW OKGIIK_11060 0.44 -1.4 2,373,584 + cheW OKGIIK_11060 0.44 +0.7 2,373,584 + cheW OKGIIK_11060 0.44 -0.5 2,373,585 - cheW OKGIIK_11060 0.44 +0.8 2,373,585 - cheW OKGIIK_11060 0.44 -0.2 2,373,585 - cheW OKGIIK_11060 0.44 -2.3 2,373,585 - cheW OKGIIK_11060 0.44 -2.1 2,373,585 - cheW OKGIIK_11060 0.44 +1.1 2,373,585 - cheW OKGIIK_11060 0.44 +0.6 2,373,585 - cheW OKGIIK_11060 0.44 -0.8 2,373,842 + -2.1 2,373,842 + +0.1 2,373,842 + +0.6 2,373,842 + -2.7 2,373,842 + +0.6 2,373,842 + +0.9 2,373,843 - -1.5 2,373,843 - -1.6 2,373,843 - -1.4 2,373,843 - +0.2 2,374,061 + ompR OKGIIK_11065 0.47 -1.9 2,374,164 + ompR OKGIIK_11065 0.75 -0.8 2,374,164 + ompR OKGIIK_11065 0.75 +0.6 2,374,164 + ompR OKGIIK_11065 0.75 -1.3 2,374,165 - ompR OKGIIK_11065 0.75 +0.2 2,374,165 - ompR OKGIIK_11065 0.75 -0.8 2,374,247 + +0.6 2,374,247 + -2.3 2,374,247 + +0.6 2,374,247 + +0.7 2,374,247 + -2.5 2,374,247 + -0.8 2,374,247 + +0.6 2,374,247 + -0.0 2,374,247 + -0.8 2,374,247 + -1.3 2,374,247 + +0.1 2,374,247 + -1.2 2,374,247 + -0.4 2,374,247 + -0.2 2,374,248 - +0.2 2,374,248 - -0.4 2,374,248 - +0.3 2,374,248 - +0.6 2,374,248 - +2.1 2,374,248 - -0.9 2,374,355 + pilG OKGIIK_11070 0.19 -0.4 2,374,355 + pilG OKGIIK_11070 0.19 -0.4 2,374,355 + pilG OKGIIK_11070 0.19 +0.1 2,374,355 + pilG OKGIIK_11070 0.19 -0.0 2,374,355 + pilG OKGIIK_11070 0.19 -1.4 2,374,355 + pilG OKGIIK_11070 0.19 +0.7 2,374,356 - pilG OKGIIK_11070 0.19 -0.3 2,374,356 - pilG OKGIIK_11070 0.19 -0.9 2,374,356 - pilG OKGIIK_11070 0.19 -0.0 2,374,356 - pilG OKGIIK_11070 0.19 +0.4 2,374,356 - pilG OKGIIK_11070 0.19 -0.6 2,374,356 - pilG OKGIIK_11070 0.19 -0.4 2,374,356 - pilG OKGIIK_11070 0.19 -2.3 2,374,356 - pilG OKGIIK_11070 0.19 -1.6 2,374,356 - pilG OKGIIK_11070 0.19 -0.8 2,374,356 - pilG OKGIIK_11070 0.19 -0.0 2,374,356 - pilG OKGIIK_11070 0.19 -0.3 2,374,356 - pilG OKGIIK_11070 0.19 -0.6 2,374,356 - pilG OKGIIK_11070 0.19 -0.4 2,374,356 - pilG OKGIIK_11070 0.19 +0.6 2,374,356 - pilG OKGIIK_11070 0.19 -0.4 2,374,356 - pilG OKGIIK_11070 0.19 -0.6 2,374,476 - pilG OKGIIK_11070 0.49 +0.1 2,374,573 + pilG OKGIIK_11070 0.74 -1.3 2,374,573 + pilG OKGIIK_11070 0.74 -0.7 2,374,573 + pilG OKGIIK_11070 0.74 -2.4 2,374,573 + pilG OKGIIK_11070 0.74 +0.3 2,374,573 + pilG OKGIIK_11070 0.74 +0.7 2,374,574 - pilG OKGIIK_11070 0.74 +0.1 2,374,574 - pilG OKGIIK_11070 0.74 +1.9 2,374,574 - pilG OKGIIK_11070 0.74 -2.7 2,374,574 - pilG OKGIIK_11070 0.74 -0.0 2,374,574 - pilG OKGIIK_11070 0.74 -1.4 2,374,574 - pilG OKGIIK_11070 0.74 +0.1 2,374,574 - pilG OKGIIK_11070 0.74 -0.5 2,374,574 - pilG OKGIIK_11070 0.74 -1.0 2,374,668 + -0.5 2,374,668 + -0.9 2,374,668 + -0.0 2,374,668 + -0.6 2,374,669 - -0.5 2,374,669 - -0.6 2,374,669 - -2.0 2,374,669 - +0.3 2,374,669 - +0.1 2,374,691 + -0.4 2,374,691 + -1.3 2,374,691 + -0.0 2,374,691 + -0.2 2,374,691 + -1.3 2,374,691 + +0.8 2,374,691 + -1.9 2,374,691 + -0.3 2,374,691 + +1.1 2,374,691 + +0.6 2,374,691 + -1.8 2,374,691 + +0.4 2,374,692 - +0.8 2,374,692 - -0.9 2,374,692 - -0.0 2,374,692 - -1.0 2,374,692 - -0.8 2,374,692 - -0.9 2,374,692 - -1.8 2,374,692 - -0.0 2,374,723 + -0.4 2,374,723 + -0.9 2,374,723 + -0.6 2,374,723 + -1.3 2,374,724 - +0.3 2,374,793 + -0.4 2,374,793 + +0.2 2,374,794 - +2.1 2,374,807 - -0.7 2,374,834 - -0.6 2,374,834 - +1.1 2,374,834 - -0.1 2,374,834 - -0.3 2,374,863 + +0.0 2,374,863 + -1.0 2,374,863 + +1.3 2,374,863 + -0.7 2,374,863 + -0.6 2,374,863 + -0.1 2,374,864 - +1.4 2,374,864 - +1.9 2,374,864 - +0.3 2,374,864 - +0.1 2,374,864 - +1.2 2,374,903 + -0.4 2,374,903 + -0.7 2,374,903 + -0.3 2,374,903 + +0.1 2,374,903 + -0.8 2,374,903 + -1.4 2,374,903 + +0.2 2,374,904 - -0.0 2,374,904 - -0.8 2,374,904 - -1.1 2,374,904 - -1.4 2,374,904 - +0.6 2,374,963 + +0.4 2,374,963 + +0.2 2,374,963 + -0.2 2,374,963 + +0.2 2,374,963 + -0.5 2,374,963 + +1.1 2,374,964 - +0.2 2,374,964 - -1.7 2,374,964 - -0.8 2,374,964 - +1.0 2,374,966 + -0.9 2,374,966 + +0.6 2,374,966 + +1.1 2,374,966 + +1.3 2,374,966 + +0.9 2,374,966 + +0.1 2,374,966 + -0.8 2,374,966 + -0.3 2,374,966 + -1.4 2,374,966 + -0.8 2,374,966 + -1.0 2,374,966 + -0.3 2,374,966 + -0.9 2,374,966 + +0.6 2,374,966 + +0.1 2,374,966 + +0.1 2,374,966 + +1.8 2,374,966 + +0.6 2,374,966 + -1.4 2,374,966 + -0.1 2,374,966 + +0.8 2,374,966 + +0.7 2,374,966 + +0.3 2,374,966 + +0.3 2,374,966 + +0.3 2,374,967 - -0.1 2,374,967 - -0.1 2,374,967 - -1.5 2,374,967 - +0.0 2,374,967 - +0.6 2,374,967 - -1.0 2,374,967 - +0.7 2,374,967 - -0.8 2,374,967 - -1.4 2,374,967 - -0.7 2,374,967 - -0.6 2,374,967 - +0.3 2,374,967 - -0.7 2,374,967 - -0.8 2,374,967 - +0.8 2,374,967 - -0.2 2,374,968 + +0.1 2,374,968 + -0.4 2,374,968 + +0.9 2,374,968 + -0.8 2,374,968 + -2.4 2,374,969 - +0.2 2,374,969 - -1.2 2,374,969 - +0.5 2,374,969 - -0.8 2,375,072 + -1.4 2,375,073 - -2.0 2,375,073 - +0.6 2,375,134 + gshB OKGIIK_11075 0.11 -0.4 2,375,134 + gshB OKGIIK_11075 0.11 -1.4 2,375,135 - gshB OKGIIK_11075 0.11 -1.6 2,375,135 - gshB OKGIIK_11075 0.11 -1.8 2,375,135 - gshB OKGIIK_11075 0.11 -0.9 2,375,135 - gshB OKGIIK_11075 0.11 -1.0
Or see this region's nucleotide sequence