Strain Fitness in Rhodanobacter sp. FW510-T8 around OKGIIK_10185

Experiment: R2A_PIPES with Nickel (II) chloride 424 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntbamA and lpxD are separated by 121 nucleotideslpxD and fabZ overlap by 8 nucleotidesfabZ and lpxA overlap by 4 nucleotides OKGIIK_10180: bamA - outer membrane protein assembly factor BamA, at 2,194,668 to 2,197,085 bamA OKGIIK_10185: lpxD - UDP-3-O-(3-hydroxymyristoyl)glucosamine N-acyltransferase, at 2,197,207 to 2,198,235 lpxD OKGIIK_10190: fabZ - 3-hydroxyacyl-ACP dehydratase FabZ, at 2,198,228 to 2,198,701 fabZ OKGIIK_10195: lpxA - acyl-ACP--UDP-N-acetylglucosamine O-acyltransferase, at 2,198,698 to 2,199,471 lpxA Position (kb) 2197 2198 2199Strain fitness (log2 ratio) -2 -1 0 1at 2197.178 kb on + strandat 2197.178 kb on + strandat 2197.178 kb on + strandat 2197.178 kb on + strandat 2198.223 kb on + strandat 2198.223 kb on + strandat 2198.223 kb on + strandat 2198.223 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM
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2,197,178 + -0.1
2,197,178 + +0.2
2,197,178 + +0.9
2,197,178 + -0.2
2,198,223 + +1.0
2,198,223 + -0.0
2,198,223 + -2.7
2,198,223 + +0.7

Or see this region's nucleotide sequence