Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rpsF and iscA are separated by 183 nucleotides iscA and asnS are separated by 155 nucleotides asnS and OKGIIK_09615 are separated by 14 nucleotides OKGIIK_09615 and OKGIIK_09620 overlap by 4 nucleotides
OKGIIK_09600: rpsF - 30S ribosomal protein S6, at 2,077,814 to 2,078,242
rpsF
OKGIIK_09605: iscA - iron-sulfur cluster assembly accessory protein, at 2,078,426 to 2,078,752
iscA
OKGIIK_09610: asnS - asparagine--tRNA ligase, at 2,078,908 to 2,080,308
asnS
OKGIIK_09615: OKGIIK_09615 - Maltose operon periplasmic protein precursor (MalM), at 2,080,323 to 2,081,147
_09615
OKGIIK_09620: OKGIIK_09620 - hypothetical protein, at 2,081,144 to 2,081,458
_09620
Position (kb)
2078
2079
2080
2081 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2078.354 kb on - strand at 2078.354 kb on - strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.815 kb on + strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2078.816 kb on - strand at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.599 kb on + strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.600 kb on - strand, within OKGIIK_09615 at 2080.692 kb on + strand, within OKGIIK_09615 at 2080.692 kb on + strand, within OKGIIK_09615 at 2080.692 kb on + strand, within OKGIIK_09615 at 2080.692 kb on + strand, within OKGIIK_09615 at 2080.693 kb on - strand, within OKGIIK_09615 at 2080.693 kb on - strand, within OKGIIK_09615 at 2080.693 kb on - strand, within OKGIIK_09615 at 2080.693 kb on - strand, within OKGIIK_09615 at 2080.693 kb on - strand, within OKGIIK_09615 at 2080.693 kb on - strand, within OKGIIK_09615 at 2080.839 kb on + strand, within OKGIIK_09615 at 2080.839 kb on + strand, within OKGIIK_09615 at 2080.839 kb on + strand, within OKGIIK_09615 at 2080.839 kb on + strand, within OKGIIK_09615 at 2080.840 kb on - strand, within OKGIIK_09615 at 2080.840 kb on - strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.851 kb on + strand, within OKGIIK_09615 at 2080.852 kb on - strand, within OKGIIK_09615 at 2080.852 kb on - strand, within OKGIIK_09615 at 2080.852 kb on - strand, within OKGIIK_09615 at 2080.852 kb on - strand, within OKGIIK_09615 at 2080.852 kb on - strand, within OKGIIK_09615 at 2080.852 kb on - strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.884 kb on + strand, within OKGIIK_09615 at 2080.885 kb on - strand, within OKGIIK_09615 at 2080.885 kb on - strand, within OKGIIK_09615 at 2080.885 kb on - strand, within OKGIIK_09615 at 2081.013 kb on + strand, within OKGIIK_09615 at 2081.013 kb on + strand, within OKGIIK_09615 at 2081.013 kb on + strand, within OKGIIK_09615 at 2081.013 kb on + strand, within OKGIIK_09615 at 2081.014 kb on - strand, within OKGIIK_09615 at 2081.156 kb on + strand at 2081.156 kb on + strand at 2081.156 kb on + strand at 2081.156 kb on + strand at 2081.156 kb on + strand at 2081.157 kb on - strand at 2081.157 kb on - strand at 2081.157 kb on - strand at 2081.157 kb on - strand at 2081.157 kb on - strand at 2081.201 kb on + strand, within OKGIIK_09620 at 2081.201 kb on + strand, within OKGIIK_09620 at 2081.201 kb on + strand, within OKGIIK_09620 at 2081.201 kb on + strand, within OKGIIK_09620 at 2081.202 kb on - strand, within OKGIIK_09620 at 2081.202 kb on - strand, within OKGIIK_09620 at 2081.202 kb on - strand, within OKGIIK_09620 at 2081.202 kb on - strand, within OKGIIK_09620 at 2081.202 kb on - strand, within OKGIIK_09620
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,078,354 - +0.4 2,078,354 - -0.3 2,078,815 + +1.3 2,078,815 + +0.3 2,078,815 + -1.2 2,078,815 + +1.0 2,078,815 + -0.4 2,078,815 + -1.6 2,078,815 + -0.7 2,078,815 + -0.9 2,078,815 + -1.7 2,078,815 + -0.3 2,078,815 + -0.4 2,078,815 + -0.5 2,078,815 + -0.8 2,078,815 + -0.4 2,078,816 - +0.4 2,078,816 - -1.7 2,078,816 - +0.6 2,078,816 - +0.1 2,078,816 - +1.3 2,078,816 - -0.2 2,078,816 - -1.6 2,078,816 - +0.1 2,078,816 - -2.5 2,080,599 + OKGIIK_09615 0.33 -0.0 2,080,599 + OKGIIK_09615 0.33 +0.4 2,080,599 + OKGIIK_09615 0.33 -0.7 2,080,599 + OKGIIK_09615 0.33 -1.2 2,080,599 + OKGIIK_09615 0.33 -2.8 2,080,599 + OKGIIK_09615 0.33 -0.4 2,080,599 + OKGIIK_09615 0.33 -1.2 2,080,599 + OKGIIK_09615 0.33 +0.6 2,080,599 + OKGIIK_09615 0.33 +0.2 2,080,599 + OKGIIK_09615 0.33 -0.7 2,080,599 + OKGIIK_09615 0.33 -1.1 2,080,599 + OKGIIK_09615 0.33 +0.6 2,080,599 + OKGIIK_09615 0.33 -0.7 2,080,600 - OKGIIK_09615 0.34 -0.6 2,080,600 - OKGIIK_09615 0.34 -1.1 2,080,600 - OKGIIK_09615 0.34 -1.2 2,080,600 - OKGIIK_09615 0.34 -1.0 2,080,600 - OKGIIK_09615 0.34 -0.8 2,080,600 - OKGIIK_09615 0.34 +0.1 2,080,600 - OKGIIK_09615 0.34 +0.7 2,080,600 - OKGIIK_09615 0.34 -0.5 2,080,600 - OKGIIK_09615 0.34 -1.0 2,080,600 - OKGIIK_09615 0.34 -1.1 2,080,600 - OKGIIK_09615 0.34 +1.3 2,080,600 - OKGIIK_09615 0.34 -0.4 2,080,600 - OKGIIK_09615 0.34 -0.9 2,080,600 - OKGIIK_09615 0.34 +0.5 2,080,600 - OKGIIK_09615 0.34 -1.7 2,080,600 - OKGIIK_09615 0.34 +0.3 2,080,600 - OKGIIK_09615 0.34 +0.6 2,080,600 - OKGIIK_09615 0.34 -2.2 2,080,600 - OKGIIK_09615 0.34 +0.8 2,080,600 - OKGIIK_09615 0.34 -0.9 2,080,692 + OKGIIK_09615 0.45 -0.7 2,080,692 + OKGIIK_09615 0.45 -0.4 2,080,692 + OKGIIK_09615 0.45 +0.7 2,080,692 + OKGIIK_09615 0.45 -0.5 2,080,693 - OKGIIK_09615 0.45 -0.5 2,080,693 - OKGIIK_09615 0.45 +0.3 2,080,693 - OKGIIK_09615 0.45 -1.6 2,080,693 - OKGIIK_09615 0.45 -1.6 2,080,693 - OKGIIK_09615 0.45 -1.6 2,080,693 - OKGIIK_09615 0.45 -3.5 2,080,839 + OKGIIK_09615 0.63 -1.2 2,080,839 + OKGIIK_09615 0.63 +0.4 2,080,839 + OKGIIK_09615 0.63 -0.4 2,080,839 + OKGIIK_09615 0.63 +0.6 2,080,840 - OKGIIK_09615 0.63 +0.6 2,080,840 - OKGIIK_09615 0.63 +0.9 2,080,851 + OKGIIK_09615 0.64 -0.8 2,080,851 + OKGIIK_09615 0.64 +0.2 2,080,851 + OKGIIK_09615 0.64 -0.6 2,080,851 + OKGIIK_09615 0.64 +0.6 2,080,851 + OKGIIK_09615 0.64 -1.4 2,080,851 + OKGIIK_09615 0.64 +0.3 2,080,851 + OKGIIK_09615 0.64 -0.7 2,080,852 - OKGIIK_09615 0.64 +0.3 2,080,852 - OKGIIK_09615 0.64 -0.1 2,080,852 - OKGIIK_09615 0.64 -1.9 2,080,852 - OKGIIK_09615 0.64 -0.0 2,080,852 - OKGIIK_09615 0.64 +0.1 2,080,852 - OKGIIK_09615 0.64 -0.1 2,080,884 + OKGIIK_09615 0.68 -0.2 2,080,884 + OKGIIK_09615 0.68 -1.2 2,080,884 + OKGIIK_09615 0.68 +1.8 2,080,884 + OKGIIK_09615 0.68 +0.8 2,080,884 + OKGIIK_09615 0.68 +0.1 2,080,884 + OKGIIK_09615 0.68 -0.7 2,080,884 + OKGIIK_09615 0.68 +0.6 2,080,884 + OKGIIK_09615 0.68 -0.3 2,080,884 + OKGIIK_09615 0.68 +0.1 2,080,884 + OKGIIK_09615 0.68 -1.4 2,080,884 + OKGIIK_09615 0.68 -0.4 2,080,884 + OKGIIK_09615 0.68 +1.8 2,080,884 + OKGIIK_09615 0.68 +1.2 2,080,885 - OKGIIK_09615 0.68 -0.4 2,080,885 - OKGIIK_09615 0.68 -0.4 2,080,885 - OKGIIK_09615 0.68 -0.0 2,081,013 + OKGIIK_09615 0.84 +0.8 2,081,013 + OKGIIK_09615 0.84 -0.2 2,081,013 + OKGIIK_09615 0.84 -0.0 2,081,013 + OKGIIK_09615 0.84 -0.4 2,081,014 - OKGIIK_09615 0.84 +0.4 2,081,156 + -0.2 2,081,156 + +1.0 2,081,156 + -0.7 2,081,156 + -2.0 2,081,156 + -0.4 2,081,157 - +0.2 2,081,157 - -0.7 2,081,157 - +1.6 2,081,157 - +0.3 2,081,157 - +0.4 2,081,201 + OKGIIK_09620 0.18 +1.4 2,081,201 + OKGIIK_09620 0.18 -0.2 2,081,201 + OKGIIK_09620 0.18 -0.7 2,081,201 + OKGIIK_09620 0.18 +0.9 2,081,202 - OKGIIK_09620 0.18 -0.7 2,081,202 - OKGIIK_09620 0.18 +0.9 2,081,202 - OKGIIK_09620 0.18 -0.6 2,081,202 - OKGIIK_09620 0.18 -1.2 2,081,202 - OKGIIK_09620 0.18 -0.3
Or see this region's nucleotide sequence