Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dnaB and rplI are separated by 243 nucleotides rplI and rpsR are separated by 135 nucleotides rpsR and rpsF are separated by 14 nucleotides rpsF and iscA are separated by 183 nucleotides
OKGIIK_09585: dnaB - replicative DNA helicase, at 2,075,346 to 2,076,740
dnaB
OKGIIK_09590: rplI - 50S ribosomal protein L9, at 2,076,984 to 2,077,433
rplI
OKGIIK_09595: rpsR - 30S ribosomal protein S18, at 2,077,569 to 2,077,799
rpsR
OKGIIK_09600: rpsF - 30S ribosomal protein S6, at 2,077,814 to 2,078,242
rpsF
OKGIIK_09605: iscA - iron-sulfur cluster assembly accessory protein, at 2,078,426 to 2,078,752
iscA
Position (kb)
2076
2077
2078 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.766 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.851 kb on - strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2076.985 kb on + strand at 2077.054 kb on + strand, within rplI at 2077.055 kb on - strand, within rplI at 2077.273 kb on + strand, within rplI at 2077.274 kb on - strand, within rplI at 2077.274 kb on - strand, within rplI at 2077.349 kb on - strand, within rplI at 2077.591 kb on + strand at 2077.592 kb on - strand, within rpsR at 2077.592 kb on - strand, within rpsR at 2077.592 kb on - strand, within rpsR at 2078.354 kb on - strand at 2078.354 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 2,076,766 - +0.6 2,076,766 - -0.7 2,076,766 - -1.2 2,076,766 - -1.5 2,076,766 - -0.9 2,076,766 - +1.8 2,076,766 - -0.1 2,076,766 - -1.9 2,076,766 - +1.4 2,076,766 - -0.0 2,076,766 - -0.1 2,076,766 - -0.4 2,076,851 - +0.4 2,076,851 - -1.0 2,076,851 - -1.4 2,076,851 - +0.9 2,076,851 - +0.4 2,076,851 - -1.0 2,076,851 - -0.2 2,076,851 - -0.5 2,076,851 - +1.4 2,076,851 - -0.7 2,076,851 - +2.4 2,076,851 - -0.4 2,076,851 - -0.3 2,076,851 - +0.3 2,076,851 - -1.2 2,076,851 - -0.4 2,076,851 - +1.9 2,076,985 + -1.2 2,076,985 + -0.2 2,076,985 + -1.2 2,076,985 + +0.6 2,076,985 + +2.6 2,076,985 + -0.7 2,076,985 + -0.0 2,076,985 + -1.2 2,076,985 + -0.4 2,076,985 + +0.3 2,076,985 + +1.3 2,076,985 + -0.7 2,076,985 + +0.5 2,076,985 + +0.5 2,076,985 + -1.4 2,076,985 + +0.3 2,076,985 + -1.7 2,076,985 + -1.2 2,076,985 + -0.4 2,076,985 + -0.7 2,077,054 + rplI OKGIIK_09590 0.16 -0.6 2,077,055 - rplI OKGIIK_09590 0.16 -3.1 2,077,273 + rplI OKGIIK_09590 0.64 +0.2 2,077,274 - rplI OKGIIK_09590 0.64 -0.7 2,077,274 - rplI OKGIIK_09590 0.64 -0.6 2,077,349 - rplI OKGIIK_09590 0.81 -2.5 2,077,591 + -0.7 2,077,592 - rpsR OKGIIK_09595 0.10 -1.4 2,077,592 - rpsR OKGIIK_09595 0.10 -1.2 2,077,592 - rpsR OKGIIK_09595 0.10 -0.7 2,078,354 - +0.4 2,078,354 - -0.3
Or see this region's nucleotide sequence