Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hyfB and hyfC overlap by 4 nucleotides hyfC and hyfE are separated by 0 nucleotides hyfE and hyfB are separated by 1 nucleotides
OKGIIK_07585: hyfB - hydrogenase 4 subunit B, at 1,676,192 to 1,678,213
hyfB
OKGIIK_07590: hyfC - formate hydrogenlyase, at 1,678,210 to 1,679,163
hyfC
OKGIIK_07595: hyfE - formate hydrogenlyase, at 1,679,164 to 1,679,832
hyfE
OKGIIK_07600: hyfB - hydrogenase 4 subunit F, at 1,679,834 to 1,681,285
hyfB
Position (kb) 1678
1679
1680 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1677.569 kb on + strand, within hyfB at 1677.569 kb on + strand, within hyfB at 1677.569 kb on + strand, within hyfB at 1677.569 kb on + strand, within hyfB at 1677.569 kb on + strand, within hyfB at 1677.570 kb on - strand, within hyfB at 1677.570 kb on - strand, within hyfB at 1677.570 kb on - strand, within hyfB at 1677.570 kb on - strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.579 kb on + strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.580 kb on - strand, within hyfB at 1677.585 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.806 kb on + strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.807 kb on - strand, within hyfB at 1677.867 kb on + strand, within hyfB at 1677.867 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.878 kb on + strand, within hyfB at 1677.879 kb on - strand, within hyfB at 1677.879 kb on - strand, within hyfB at 1677.879 kb on - strand, within hyfB at 1677.879 kb on - strand, within hyfB at 1677.879 kb on - strand, within hyfB at 1678.085 kb on + strand at 1678.086 kb on - strand at 1678.086 kb on - strand at 1678.086 kb on - strand at 1678.160 kb on + strand at 1678.160 kb on + strand at 1678.160 kb on + strand at 1678.160 kb on + strand at 1678.160 kb on + strand at 1678.160 kb on + strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.161 kb on - strand at 1678.169 kb on + strand at 1678.169 kb on + strand at 1678.170 kb on - strand at 1678.170 kb on - strand at 1678.191 kb on + strand at 1678.191 kb on + strand at 1678.191 kb on + strand at 1678.192 kb on - strand at 1678.192 kb on - strand at 1678.192 kb on - strand at 1678.250 kb on + strand at 1678.251 kb on - strand at 1678.251 kb on - strand at 1678.290 kb on - strand at 1678.345 kb on + strand, within hyfC at 1678.345 kb on + strand, within hyfC at 1678.424 kb on - strand, within hyfC at 1678.424 kb on - strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.921 kb on + strand, within hyfC at 1678.922 kb on - strand, within hyfC at 1678.922 kb on - strand, within hyfC at 1678.922 kb on - strand, within hyfC at 1678.922 kb on - strand, within hyfC at 1679.147 kb on + strand at 1679.147 kb on + strand at 1679.147 kb on + strand at 1679.147 kb on + strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.148 kb on - strand at 1679.350 kb on + strand, within hyfE at 1679.350 kb on + strand, within hyfE at 1679.350 kb on + strand, within hyfE at 1679.350 kb on + strand, within hyfE at 1679.350 kb on + strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.351 kb on - strand, within hyfE at 1679.353 kb on + strand, within hyfE at 1679.353 kb on + strand, within hyfE at 1679.353 kb on + strand, within hyfE at 1679.354 kb on - strand, within hyfE at 1679.354 kb on - strand, within hyfE at 1679.354 kb on - strand, within hyfE at 1679.689 kb on + strand, within hyfE at 1679.690 kb on - strand, within hyfE at 1679.857 kb on + strand at 1679.967 kb on + strand at 1679.968 kb on - strand at 1680.095 kb on + strand, within hyfB at 1680.095 kb on + strand, within hyfB at 1680.095 kb on + strand, within hyfB at 1680.095 kb on + strand, within hyfB at 1680.095 kb on + strand, within hyfB at 1680.095 kb on + strand, within hyfB at 1680.096 kb on - strand, within hyfB at 1680.096 kb on - strand, within hyfB at 1680.096 kb on - strand, within hyfB at 1680.096 kb on - strand, within hyfB at 1680.103 kb on + strand, within hyfB at 1680.104 kb on - strand, within hyfB at 1680.150 kb on - strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.161 kb on + strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB at 1680.162 kb on - strand, within hyfB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,677,569 + hyfB OKGIIK_07585 0.68 +1.3 1,677,569 + hyfB OKGIIK_07585 0.68 -1.2 1,677,569 + hyfB OKGIIK_07585 0.68 +0.3 1,677,569 + hyfB OKGIIK_07585 0.68 -1.7 1,677,569 + hyfB OKGIIK_07585 0.68 -1.7 1,677,570 - hyfB OKGIIK_07585 0.68 -1.2 1,677,570 - hyfB OKGIIK_07585 0.68 -0.9 1,677,570 - hyfB OKGIIK_07585 0.68 -1.4 1,677,570 - hyfB OKGIIK_07585 0.68 -1.3 1,677,579 + hyfB OKGIIK_07585 0.69 -1.0 1,677,579 + hyfB OKGIIK_07585 0.69 -0.4 1,677,579 + hyfB OKGIIK_07585 0.69 +1.0 1,677,579 + hyfB OKGIIK_07585 0.69 -0.6 1,677,579 + hyfB OKGIIK_07585 0.69 -0.5 1,677,579 + hyfB OKGIIK_07585 0.69 -0.7 1,677,579 + hyfB OKGIIK_07585 0.69 -2.0 1,677,580 - hyfB OKGIIK_07585 0.69 -0.4 1,677,580 - hyfB OKGIIK_07585 0.69 -1.1 1,677,580 - hyfB OKGIIK_07585 0.69 +1.7 1,677,580 - hyfB OKGIIK_07585 0.69 +1.1 1,677,580 - hyfB OKGIIK_07585 0.69 -1.0 1,677,580 - hyfB OKGIIK_07585 0.69 +1.6 1,677,580 - hyfB OKGIIK_07585 0.69 -0.6 1,677,585 + hyfB OKGIIK_07585 0.69 -0.4 1,677,806 + hyfB OKGIIK_07585 0.80 -0.4 1,677,806 + hyfB OKGIIK_07585 0.80 -0.2 1,677,806 + hyfB OKGIIK_07585 0.80 +0.4 1,677,806 + hyfB OKGIIK_07585 0.80 -0.1 1,677,806 + hyfB OKGIIK_07585 0.80 -1.0 1,677,806 + hyfB OKGIIK_07585 0.80 +0.3 1,677,806 + hyfB OKGIIK_07585 0.80 +1.4 1,677,806 + hyfB OKGIIK_07585 0.80 -0.5 1,677,806 + hyfB OKGIIK_07585 0.80 -0.4 1,677,806 + hyfB OKGIIK_07585 0.80 -1.4 1,677,806 + hyfB OKGIIK_07585 0.80 +0.5 1,677,806 + hyfB OKGIIK_07585 0.80 +1.2 1,677,806 + hyfB OKGIIK_07585 0.80 -0.9 1,677,806 + hyfB OKGIIK_07585 0.80 +1.8 1,677,806 + hyfB OKGIIK_07585 0.80 +0.2 1,677,806 + hyfB OKGIIK_07585 0.80 +0.1 1,677,806 + hyfB OKGIIK_07585 0.80 -0.5 1,677,806 + hyfB OKGIIK_07585 0.80 -0.2 1,677,807 - hyfB OKGIIK_07585 0.80 -1.2 1,677,807 - hyfB OKGIIK_07585 0.80 +1.5 1,677,807 - hyfB OKGIIK_07585 0.80 +0.6 1,677,807 - hyfB OKGIIK_07585 0.80 +1.8 1,677,807 - hyfB OKGIIK_07585 0.80 +0.6 1,677,807 - hyfB OKGIIK_07585 0.80 -0.7 1,677,807 - hyfB OKGIIK_07585 0.80 -1.2 1,677,807 - hyfB OKGIIK_07585 0.80 +0.0 1,677,867 + hyfB OKGIIK_07585 0.83 -2.4 1,677,867 + hyfB OKGIIK_07585 0.83 +0.0 1,677,878 + hyfB OKGIIK_07585 0.83 -1.6 1,677,878 + hyfB OKGIIK_07585 0.83 +0.3 1,677,878 + hyfB OKGIIK_07585 0.83 -1.2 1,677,878 + hyfB OKGIIK_07585 0.83 -1.9 1,677,878 + hyfB OKGIIK_07585 0.83 +0.9 1,677,878 + hyfB OKGIIK_07585 0.83 +0.6 1,677,878 + hyfB OKGIIK_07585 0.83 -1.3 1,677,879 - hyfB OKGIIK_07585 0.83 -0.3 1,677,879 - hyfB OKGIIK_07585 0.83 +0.6 1,677,879 - hyfB OKGIIK_07585 0.83 +0.1 1,677,879 - hyfB OKGIIK_07585 0.83 -0.7 1,677,879 - hyfB OKGIIK_07585 0.83 -1.2 1,678,085 + -1.2 1,678,086 - -1.6 1,678,086 - +0.6 1,678,086 - +0.9 1,678,160 + +0.4 1,678,160 + -0.6 1,678,160 + -0.3 1,678,160 + -2.2 1,678,160 + +1.2 1,678,160 + -0.2 1,678,161 - -0.9 1,678,161 - -0.4 1,678,161 - +0.6 1,678,161 - +0.6 1,678,161 - -0.3 1,678,161 - +0.3 1,678,161 - +1.3 1,678,161 - -0.1 1,678,161 - -1.1 1,678,161 - -0.7 1,678,169 + -2.6 1,678,169 + +0.0 1,678,170 - +0.6 1,678,170 - +1.3 1,678,191 + -0.6 1,678,191 + -2.0 1,678,191 + -2.6 1,678,192 - +0.0 1,678,192 - -0.5 1,678,192 - -0.7 1,678,250 + +0.1 1,678,251 - -0.0 1,678,251 - +0.2 1,678,290 - +2.4 1,678,345 + hyfC OKGIIK_07590 0.14 +1.2 1,678,345 + hyfC OKGIIK_07590 0.14 +0.6 1,678,424 - hyfC OKGIIK_07590 0.22 -1.4 1,678,424 - hyfC OKGIIK_07590 0.22 +0.6 1,678,921 + hyfC OKGIIK_07590 0.75 +0.9 1,678,921 + hyfC OKGIIK_07590 0.75 +1.0 1,678,921 + hyfC OKGIIK_07590 0.75 +0.0 1,678,921 + hyfC OKGIIK_07590 0.75 -1.0 1,678,921 + hyfC OKGIIK_07590 0.75 -1.0 1,678,921 + hyfC OKGIIK_07590 0.75 -1.0 1,678,921 + hyfC OKGIIK_07590 0.75 +0.9 1,678,921 + hyfC OKGIIK_07590 0.75 +1.0 1,678,921 + hyfC OKGIIK_07590 0.75 -1.0 1,678,921 + hyfC OKGIIK_07590 0.75 -0.4 1,678,921 + hyfC OKGIIK_07590 0.75 -1.5 1,678,921 + hyfC OKGIIK_07590 0.75 -1.2 1,678,922 - hyfC OKGIIK_07590 0.75 -1.4 1,678,922 - hyfC OKGIIK_07590 0.75 -0.1 1,678,922 - hyfC OKGIIK_07590 0.75 +1.1 1,678,922 - hyfC OKGIIK_07590 0.75 +0.6 1,679,147 + -1.1 1,679,147 + -1.2 1,679,147 + -1.2 1,679,147 + -0.5 1,679,148 - +0.4 1,679,148 - +0.8 1,679,148 - -0.1 1,679,148 - -0.7 1,679,148 - -1.0 1,679,148 - -0.3 1,679,148 - +1.2 1,679,148 - -0.1 1,679,148 - -0.1 1,679,148 - +0.9 1,679,350 + hyfE OKGIIK_07595 0.28 -2.2 1,679,350 + hyfE OKGIIK_07595 0.28 +1.9 1,679,350 + hyfE OKGIIK_07595 0.28 -1.3 1,679,350 + hyfE OKGIIK_07595 0.28 +0.7 1,679,350 + hyfE OKGIIK_07595 0.28 -0.8 1,679,351 - hyfE OKGIIK_07595 0.28 -2.6 1,679,351 - hyfE OKGIIK_07595 0.28 +1.6 1,679,351 - hyfE OKGIIK_07595 0.28 -0.1 1,679,351 - hyfE OKGIIK_07595 0.28 -0.5 1,679,351 - hyfE OKGIIK_07595 0.28 +0.8 1,679,351 - hyfE OKGIIK_07595 0.28 -2.1 1,679,351 - hyfE OKGIIK_07595 0.28 -1.4 1,679,353 + hyfE OKGIIK_07595 0.28 -0.2 1,679,353 + hyfE OKGIIK_07595 0.28 +0.5 1,679,353 + hyfE OKGIIK_07595 0.28 -3.0 1,679,354 - hyfE OKGIIK_07595 0.28 +1.6 1,679,354 - hyfE OKGIIK_07595 0.28 +1.6 1,679,354 - hyfE OKGIIK_07595 0.28 +1.0 1,679,689 + hyfE OKGIIK_07595 0.78 +0.5 1,679,690 - hyfE OKGIIK_07595 0.79 -2.1 1,679,857 + -1.2 1,679,967 + -0.5 1,679,968 - -0.4 1,680,095 + hyfB OKGIIK_07600 0.18 +0.6 1,680,095 + hyfB OKGIIK_07600 0.18 -0.7 1,680,095 + hyfB OKGIIK_07600 0.18 +0.8 1,680,095 + hyfB OKGIIK_07600 0.18 -1.0 1,680,095 + hyfB OKGIIK_07600 0.18 -0.6 1,680,095 + hyfB OKGIIK_07600 0.18 +0.2 1,680,096 - hyfB OKGIIK_07600 0.18 +0.1 1,680,096 - hyfB OKGIIK_07600 0.18 +0.5 1,680,096 - hyfB OKGIIK_07600 0.18 +0.6 1,680,096 - hyfB OKGIIK_07600 0.18 +0.9 1,680,103 + hyfB OKGIIK_07600 0.19 +0.3 1,680,104 - hyfB OKGIIK_07600 0.19 +0.8 1,680,150 - hyfB OKGIIK_07600 0.22 +0.1 1,680,161 + hyfB OKGIIK_07600 0.23 -0.1 1,680,161 + hyfB OKGIIK_07600 0.23 -0.1 1,680,161 + hyfB OKGIIK_07600 0.23 +0.8 1,680,161 + hyfB OKGIIK_07600 0.23 +1.3 1,680,161 + hyfB OKGIIK_07600 0.23 -0.4 1,680,161 + hyfB OKGIIK_07600 0.23 -0.9 1,680,161 + hyfB OKGIIK_07600 0.23 -1.0 1,680,161 + hyfB OKGIIK_07600 0.23 +1.7 1,680,161 + hyfB OKGIIK_07600 0.23 -0.7 1,680,161 + hyfB OKGIIK_07600 0.23 +0.3 1,680,161 + hyfB OKGIIK_07600 0.23 -1.0 1,680,161 + hyfB OKGIIK_07600 0.23 -0.1 1,680,161 + hyfB OKGIIK_07600 0.23 -0.5 1,680,161 + hyfB OKGIIK_07600 0.23 +0.6 1,680,162 - hyfB OKGIIK_07600 0.23 -0.3 1,680,162 - hyfB OKGIIK_07600 0.23 +0.2 1,680,162 - hyfB OKGIIK_07600 0.23 +0.0 1,680,162 - hyfB OKGIIK_07600 0.23 -0.3 1,680,162 - hyfB OKGIIK_07600 0.23 -0.7 1,680,162 - hyfB OKGIIK_07600 0.23 -0.3 1,680,162 - hyfB OKGIIK_07600 0.23 -0.7 1,680,162 - hyfB OKGIIK_07600 0.23 +0.8 1,680,162 - hyfB OKGIIK_07600 0.23 +0.7 1,680,162 - hyfB OKGIIK_07600 0.23 +0.3
Or see this region's nucleotide sequence