Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt lysR and greA are separated by 292 nucleotides greA and carB overlap by 4 nucleotides carB and OKGIIK_07300 are separated by 1 nucleotides
OKGIIK_07285: lysR - LysR family transcriptional regulator, at 1,610,856 to 1,611,767
lysR
OKGIIK_07290: greA - transcription elongation factor GreA, at 1,612,060 to 1,612,536
greA
OKGIIK_07295: carB - carbamoyl-phosphate synthase large subunit, at 1,612,533 to 1,615,760
carB
OKGIIK_07300: OKGIIK_07300 - carbamoyl phosphate synthase small subunit, at 1,615,762 to 1,616,889
_07300
Position (kb)
1612
1613
1614
1615
1616 Strain fitness (log2 ratio)
-3
-2
-1
0
1 at 1611.639 kb on + strand, within lysR at 1611.640 kb on - strand, within lysR at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.067 kb on + strand at 1612.068 kb on - strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.088 kb on + strand at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.223 kb on + strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.224 kb on - strand, within greA at 1612.394 kb on + strand, within greA at 1612.394 kb on + strand, within greA at 1612.395 kb on - strand, within greA at 1612.395 kb on - strand, within greA at 1612.639 kb on + strand at 1612.640 kb on - strand at 1612.640 kb on - strand at 1612.723 kb on + strand at 1612.724 kb on - strand at 1613.463 kb on + strand, within carB at 1613.464 kb on - strand, within carB at 1613.552 kb on - strand, within carB at 1613.552 kb on - strand, within carB at 1613.575 kb on + strand, within carB at 1613.608 kb on + strand, within carB at 1613.609 kb on - strand, within carB at 1613.609 kb on - strand, within carB at 1613.911 kb on + strand, within carB at 1614.118 kb on + strand, within carB at 1614.127 kb on + strand, within carB at 1614.181 kb on + strand, within carB at 1614.400 kb on + strand, within carB at 1614.401 kb on - strand, within carB at 1614.401 kb on - strand, within carB at 1614.701 kb on - strand, within carB at 1615.213 kb on + strand, within carB at 1615.430 kb on - strand, within carB at 1615.764 kb on - strand at 1615.764 kb on - strand at 1615.764 kb on - strand at 1615.805 kb on + strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.806 kb on - strand at 1615.911 kb on + strand, within OKGIIK_07300 at 1615.911 kb on + strand, within OKGIIK_07300 at 1615.912 kb on - strand, within OKGIIK_07300 at 1616.151 kb on - strand, within OKGIIK_07300 at 1616.366 kb on + strand, within OKGIIK_07300 at 1616.747 kb on + strand, within OKGIIK_07300
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,611,639 + lysR OKGIIK_07285 0.86 -1.0 1,611,640 - lysR OKGIIK_07285 0.86 -0.8 1,612,067 + +0.5 1,612,067 + -1.2 1,612,067 + -0.6 1,612,067 + +0.5 1,612,067 + -1.0 1,612,067 + -0.2 1,612,067 + -0.7 1,612,068 - -0.5 1,612,088 + -1.2 1,612,088 + -0.7 1,612,088 + -1.4 1,612,088 + -1.0 1,612,223 + greA OKGIIK_07290 0.34 -0.3 1,612,223 + greA OKGIIK_07290 0.34 +0.7 1,612,223 + greA OKGIIK_07290 0.34 -0.0 1,612,223 + greA OKGIIK_07290 0.34 -2.7 1,612,223 + greA OKGIIK_07290 0.34 +0.3 1,612,224 - greA OKGIIK_07290 0.34 -0.6 1,612,224 - greA OKGIIK_07290 0.34 +1.2 1,612,224 - greA OKGIIK_07290 0.34 +1.2 1,612,224 - greA OKGIIK_07290 0.34 -0.4 1,612,394 + greA OKGIIK_07290 0.70 +0.9 1,612,394 + greA OKGIIK_07290 0.70 -0.8 1,612,395 - greA OKGIIK_07290 0.70 +0.3 1,612,395 - greA OKGIIK_07290 0.70 +0.2 1,612,639 + -1.6 1,612,640 - -0.4 1,612,640 - -0.4 1,612,723 + -2.3 1,612,724 - -1.2 1,613,463 + carB OKGIIK_07295 0.29 -0.4 1,613,464 - carB OKGIIK_07295 0.29 -1.6 1,613,552 - carB OKGIIK_07295 0.32 +0.3 1,613,552 - carB OKGIIK_07295 0.32 -1.2 1,613,575 + carB OKGIIK_07295 0.32 -3.0 1,613,608 + carB OKGIIK_07295 0.33 -0.4 1,613,609 - carB OKGIIK_07295 0.33 -0.4 1,613,609 - carB OKGIIK_07295 0.33 -0.4 1,613,911 + carB OKGIIK_07295 0.43 -1.4 1,614,118 + carB OKGIIK_07295 0.49 -0.5 1,614,127 + carB OKGIIK_07295 0.49 -0.4 1,614,181 + carB OKGIIK_07295 0.51 -1.0 1,614,400 + carB OKGIIK_07295 0.58 +0.4 1,614,401 - carB OKGIIK_07295 0.58 -2.6 1,614,401 - carB OKGIIK_07295 0.58 -1.6 1,614,701 - carB OKGIIK_07295 0.67 -1.0 1,615,213 + carB OKGIIK_07295 0.83 -1.4 1,615,430 - carB OKGIIK_07295 0.90 -0.4 1,615,764 - -0.5 1,615,764 - +0.4 1,615,764 - -2.2 1,615,805 + -0.7 1,615,806 - -1.6 1,615,806 - -2.1 1,615,806 - -1.8 1,615,806 - -3.2 1,615,911 + OKGIIK_07300 0.13 -1.7 1,615,911 + OKGIIK_07300 0.13 -0.4 1,615,912 - OKGIIK_07300 0.13 -1.7 1,616,151 - OKGIIK_07300 0.34 -1.4 1,616,366 + OKGIIK_07300 0.54 -3.1 1,616,747 + OKGIIK_07300 0.87 -2.3
Or see this region's nucleotide sequence