Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt merR and OKGIIK_06090 are separated by 17 nucleotides OKGIIK_06090 and OKGIIK_06095 are separated by 111 nucleotides OKGIIK_06095 and OKGIIK_06100 are separated by 216 nucleotides OKGIIK_06100 and OKGIIK_06105 are separated by 11 nucleotides
OKGIIK_06085: merR - Hg(II)-responsive transcriptional regulator, at 1,343,553 to 1,343,960
merR
OKGIIK_06090: OKGIIK_06090 - hypothetical protein, at 1,343,978 to 1,344,619
_06090
OKGIIK_06095: OKGIIK_06095 - (2Fe-2S)-binding protein, at 1,344,731 to 1,344,952
_06095
OKGIIK_06100: OKGIIK_06100 - DUF2282 domain-containing protein, at 1,345,169 to 1,345,453
_06100
OKGIIK_06105: OKGIIK_06105 - UPF0276 protein CV_3513, at 1,345,465 to 1,346,337
_06105
Position (kb)
1344
1345 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1343.816 kb on + strand, within merR at 1343.816 kb on + strand, within merR at 1343.817 kb on - strand, within merR at 1343.843 kb on - strand, within merR at 1343.882 kb on - strand, within merR at 1343.991 kb on + strand at 1343.996 kb on + strand at 1343.996 kb on + strand at 1343.996 kb on + strand at 1343.996 kb on + strand at 1343.996 kb on + strand at 1343.997 kb on - strand at 1343.997 kb on - strand at 1343.997 kb on - strand at 1343.997 kb on - strand at 1343.997 kb on - strand at 1344.041 kb on + strand at 1344.041 kb on + strand at 1344.042 kb on - strand at 1344.133 kb on + strand, within OKGIIK_06090 at 1344.133 kb on + strand, within OKGIIK_06090 at 1344.133 kb on + strand, within OKGIIK_06090 at 1344.134 kb on - strand, within OKGIIK_06090 at 1344.261 kb on + strand, within OKGIIK_06090 at 1344.311 kb on + strand, within OKGIIK_06090 at 1344.311 kb on + strand, within OKGIIK_06090 at 1344.311 kb on + strand, within OKGIIK_06090 at 1344.312 kb on - strand, within OKGIIK_06090 at 1344.312 kb on - strand, within OKGIIK_06090 at 1344.312 kb on - strand, within OKGIIK_06090 at 1344.312 kb on - strand, within OKGIIK_06090 at 1344.456 kb on + strand, within OKGIIK_06090 at 1344.647 kb on + strand at 1344.698 kb on - strand at 1344.879 kb on + strand, within OKGIIK_06095 at 1344.879 kb on + strand, within OKGIIK_06095 at 1344.880 kb on - strand, within OKGIIK_06095 at 1344.950 kb on + strand at 1344.950 kb on + strand at 1344.950 kb on + strand at 1344.951 kb on - strand at 1344.951 kb on - strand at 1345.002 kb on - strand at 1345.017 kb on + strand at 1345.018 kb on - strand at 1345.018 kb on - strand at 1345.018 kb on - strand at 1345.153 kb on + strand at 1345.153 kb on + strand at 1345.153 kb on + strand at 1345.153 kb on + strand at 1345.154 kb on - strand at 1345.154 kb on - strand at 1345.154 kb on - strand at 1345.154 kb on - strand at 1345.175 kb on - strand at 1345.279 kb on + strand, within OKGIIK_06100 at 1345.279 kb on + strand, within OKGIIK_06100 at 1345.279 kb on + strand, within OKGIIK_06100 at 1345.279 kb on + strand, within OKGIIK_06100 at 1345.279 kb on + strand, within OKGIIK_06100 at 1345.279 kb on + strand, within OKGIIK_06100 at 1345.280 kb on - strand, within OKGIIK_06100 at 1345.859 kb on + strand, within OKGIIK_06105 at 1345.859 kb on + strand, within OKGIIK_06105 at 1345.859 kb on + strand, within OKGIIK_06105 at 1345.860 kb on - strand, within OKGIIK_06105 at 1345.927 kb on + strand, within OKGIIK_06105 at 1345.927 kb on + strand, within OKGIIK_06105 at 1345.927 kb on + strand, within OKGIIK_06105 at 1345.928 kb on - strand, within OKGIIK_06105 at 1345.928 kb on - strand, within OKGIIK_06105 at 1345.928 kb on - strand, within OKGIIK_06105 at 1345.928 kb on - strand, within OKGIIK_06105
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,343,816 + merR OKGIIK_06085 0.64 -1.4 1,343,816 + merR OKGIIK_06085 0.64 -0.4 1,343,817 - merR OKGIIK_06085 0.65 -0.2 1,343,843 - merR OKGIIK_06085 0.71 -0.8 1,343,882 - merR OKGIIK_06085 0.81 +0.5 1,343,991 + -0.8 1,343,996 + +0.5 1,343,996 + -1.9 1,343,996 + -0.0 1,343,996 + +1.9 1,343,996 + +1.1 1,343,997 - -2.8 1,343,997 - +1.0 1,343,997 - -1.6 1,343,997 - +0.0 1,343,997 - -1.7 1,344,041 + -2.5 1,344,041 + +0.5 1,344,042 - +0.3 1,344,133 + OKGIIK_06090 0.24 +1.2 1,344,133 + OKGIIK_06090 0.24 -1.7 1,344,133 + OKGIIK_06090 0.24 -0.7 1,344,134 - OKGIIK_06090 0.24 -0.1 1,344,261 + OKGIIK_06090 0.44 +0.2 1,344,311 + OKGIIK_06090 0.52 -0.7 1,344,311 + OKGIIK_06090 0.52 -1.0 1,344,311 + OKGIIK_06090 0.52 -1.9 1,344,312 - OKGIIK_06090 0.52 -1.6 1,344,312 - OKGIIK_06090 0.52 -0.7 1,344,312 - OKGIIK_06090 0.52 -1.2 1,344,312 - OKGIIK_06090 0.52 -0.4 1,344,456 + OKGIIK_06090 0.74 -2.4 1,344,647 + +0.3 1,344,698 - -0.6 1,344,879 + OKGIIK_06095 0.67 -0.9 1,344,879 + OKGIIK_06095 0.67 -0.8 1,344,880 - OKGIIK_06095 0.67 -0.7 1,344,950 + -0.9 1,344,950 + +0.0 1,344,950 + +0.1 1,344,951 - -1.6 1,344,951 - +1.0 1,345,002 - -1.6 1,345,017 + +0.0 1,345,018 - +0.9 1,345,018 - +1.4 1,345,018 - -0.1 1,345,153 + +0.6 1,345,153 + -2.0 1,345,153 + -0.5 1,345,153 + -1.2 1,345,154 - -0.9 1,345,154 - -0.6 1,345,154 - -2.3 1,345,154 - +0.3 1,345,175 - -0.3 1,345,279 + OKGIIK_06100 0.39 -2.3 1,345,279 + OKGIIK_06100 0.39 -0.6 1,345,279 + OKGIIK_06100 0.39 -2.2 1,345,279 + OKGIIK_06100 0.39 +0.7 1,345,279 + OKGIIK_06100 0.39 -0.0 1,345,279 + OKGIIK_06100 0.39 -0.3 1,345,280 - OKGIIK_06100 0.39 +0.3 1,345,859 + OKGIIK_06105 0.45 -1.4 1,345,859 + OKGIIK_06105 0.45 +0.3 1,345,859 + OKGIIK_06105 0.45 -0.0 1,345,860 - OKGIIK_06105 0.45 +1.2 1,345,927 + OKGIIK_06105 0.53 +0.6 1,345,927 + OKGIIK_06105 0.53 -1.5 1,345,927 + OKGIIK_06105 0.53 +0.6 1,345,928 - OKGIIK_06105 0.53 -0.1 1,345,928 - OKGIIK_06105 0.53 -0.3 1,345,928 - OKGIIK_06105 0.53 -0.1 1,345,928 - OKGIIK_06105 0.53 +0.0
Or see this region's nucleotide sequence