Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt phaC and mtnA are separated by 52 nucleotides mtnA and OKGIIK_05890 overlap by 4 nucleotides OKGIIK_05890 and gyrA are separated by 138 nucleotides
OKGIIK_05880: phaC - class III poly(R)-hydroxyalkanoic acid synthase subunit PhaC, at 1,289,112 to 1,290,179
phaC
OKGIIK_05885: mtnA - S-methyl-5-thioribose-1-phosphate isomerase, at 1,290,232 to 1,291,248
mtnA
OKGIIK_05890: OKGIIK_05890 - GtrA domain-containing protein, at 1,291,245 to 1,291,658
_05890
OKGIIK_05895: gyrA - DNA gyrase subunit A, at 1,291,797 to 1,294,400
gyrA
Position (kb) 1290
1291
1292 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1289.250 kb on + strand, within phaC at 1289.250 kb on + strand, within phaC at 1289.250 kb on + strand, within phaC at 1289.250 kb on + strand, within phaC at 1289.251 kb on - strand, within phaC at 1289.251 kb on - strand, within phaC at 1289.251 kb on - strand, within phaC at 1289.272 kb on + strand, within phaC at 1289.272 kb on + strand, within phaC at 1289.272 kb on + strand, within phaC at 1289.272 kb on + strand, within phaC at 1289.272 kb on + strand, within phaC at 1289.272 kb on + strand, within phaC at 1289.273 kb on - strand, within phaC at 1289.273 kb on - strand, within phaC at 1289.273 kb on - strand, within phaC at 1289.329 kb on - strand, within phaC at 1289.329 kb on - strand, within phaC at 1289.415 kb on + strand, within phaC at 1289.415 kb on + strand, within phaC at 1289.415 kb on + strand, within phaC at 1289.415 kb on + strand, within phaC at 1289.416 kb on - strand, within phaC at 1289.416 kb on - strand, within phaC at 1289.416 kb on - strand, within phaC at 1289.416 kb on - strand, within phaC at 1289.472 kb on + strand, within phaC at 1289.472 kb on + strand, within phaC at 1289.472 kb on + strand, within phaC at 1289.472 kb on + strand, within phaC at 1289.472 kb on + strand, within phaC at 1289.473 kb on - strand, within phaC at 1289.484 kb on + strand, within phaC at 1289.484 kb on + strand, within phaC at 1289.484 kb on + strand, within phaC at 1289.580 kb on + strand, within phaC at 1289.580 kb on + strand, within phaC at 1289.580 kb on + strand, within phaC at 1289.581 kb on - strand, within phaC at 1289.581 kb on - strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.739 kb on + strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.740 kb on - strand, within phaC at 1289.784 kb on + strand, within phaC at 1289.784 kb on + strand, within phaC at 1289.784 kb on + strand, within phaC at 1289.784 kb on + strand, within phaC at 1289.784 kb on + strand, within phaC at 1289.784 kb on + strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.785 kb on - strand, within phaC at 1289.910 kb on + strand, within phaC at 1289.910 kb on + strand, within phaC at 1289.911 kb on - strand, within phaC at 1289.911 kb on - strand, within phaC at 1289.911 kb on - strand, within phaC at 1289.911 kb on - strand, within phaC at 1290.072 kb on + strand, within phaC at 1290.072 kb on + strand, within phaC at 1290.073 kb on - strand at 1290.073 kb on - strand at 1290.073 kb on - strand at 1290.111 kb on + strand at 1290.111 kb on + strand at 1290.111 kb on + strand at 1290.111 kb on + strand at 1290.111 kb on + strand at 1290.111 kb on + strand at 1290.111 kb on + strand at 1290.111 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.112 kb on - strand at 1290.204 kb on + strand at 1290.204 kb on + strand at 1290.204 kb on + strand at 1290.205 kb on - strand at 1290.205 kb on - strand at 1290.205 kb on - strand at 1290.769 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.964 kb on + strand, within mtnA at 1290.965 kb on - strand, within mtnA at 1290.965 kb on - strand, within mtnA at 1290.965 kb on - strand, within mtnA at 1290.965 kb on - strand, within mtnA at 1290.965 kb on - strand, within mtnA at 1290.965 kb on - strand, within mtnA at 1290.988 kb on + strand, within mtnA at 1290.988 kb on + strand, within mtnA at 1290.988 kb on + strand, within mtnA at 1290.989 kb on - strand, within mtnA at 1291.356 kb on + strand, within OKGIIK_05890 at 1291.356 kb on + strand, within OKGIIK_05890 at 1291.356 kb on + strand, within OKGIIK_05890 at 1291.357 kb on - strand, within OKGIIK_05890 at 1291.357 kb on - strand, within OKGIIK_05890 at 1291.357 kb on - strand, within OKGIIK_05890 at 1291.357 kb on - strand, within OKGIIK_05890 at 1291.419 kb on + strand, within OKGIIK_05890 at 1291.509 kb on + strand, within OKGIIK_05890 at 1291.509 kb on + strand, within OKGIIK_05890 at 1291.510 kb on - strand, within OKGIIK_05890 at 1291.518 kb on + strand, within OKGIIK_05890 at 1291.518 kb on + strand, within OKGIIK_05890 at 1291.518 kb on + strand, within OKGIIK_05890 at 1291.518 kb on + strand, within OKGIIK_05890 at 1291.519 kb on - strand, within OKGIIK_05890 at 1291.519 kb on - strand, within OKGIIK_05890 at 1291.669 kb on + strand at 1291.670 kb on - strand at 1291.672 kb on - strand at 1291.708 kb on + strand at 1291.708 kb on + strand at 1291.708 kb on + strand at 1291.708 kb on + strand at 1291.709 kb on - strand at 1291.709 kb on - strand at 1291.709 kb on - strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand at 1291.710 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,289,250 + phaC OKGIIK_05880 0.13 -1.6 1,289,250 + phaC OKGIIK_05880 0.13 -0.6 1,289,250 + phaC OKGIIK_05880 0.13 -0.9 1,289,250 + phaC OKGIIK_05880 0.13 -0.7 1,289,251 - phaC OKGIIK_05880 0.13 -0.2 1,289,251 - phaC OKGIIK_05880 0.13 -2.1 1,289,251 - phaC OKGIIK_05880 0.13 -0.2 1,289,272 + phaC OKGIIK_05880 0.15 +0.2 1,289,272 + phaC OKGIIK_05880 0.15 -1.2 1,289,272 + phaC OKGIIK_05880 0.15 -0.2 1,289,272 + phaC OKGIIK_05880 0.15 -0.6 1,289,272 + phaC OKGIIK_05880 0.15 +0.9 1,289,272 + phaC OKGIIK_05880 0.15 +0.4 1,289,273 - phaC OKGIIK_05880 0.15 +0.0 1,289,273 - phaC OKGIIK_05880 0.15 +0.0 1,289,273 - phaC OKGIIK_05880 0.15 -0.5 1,289,329 - phaC OKGIIK_05880 0.20 -0.4 1,289,329 - phaC OKGIIK_05880 0.20 +1.2 1,289,415 + phaC OKGIIK_05880 0.28 +0.6 1,289,415 + phaC OKGIIK_05880 0.28 +0.2 1,289,415 + phaC OKGIIK_05880 0.28 -1.4 1,289,415 + phaC OKGIIK_05880 0.28 -2.2 1,289,416 - phaC OKGIIK_05880 0.28 +0.0 1,289,416 - phaC OKGIIK_05880 0.28 -1.6 1,289,416 - phaC OKGIIK_05880 0.28 +0.2 1,289,416 - phaC OKGIIK_05880 0.28 -0.8 1,289,472 + phaC OKGIIK_05880 0.34 -0.7 1,289,472 + phaC OKGIIK_05880 0.34 +0.6 1,289,472 + phaC OKGIIK_05880 0.34 -0.4 1,289,472 + phaC OKGIIK_05880 0.34 -1.2 1,289,472 + phaC OKGIIK_05880 0.34 -1.7 1,289,473 - phaC OKGIIK_05880 0.34 -1.3 1,289,484 + phaC OKGIIK_05880 0.35 +0.0 1,289,484 + phaC OKGIIK_05880 0.35 -0.7 1,289,484 + phaC OKGIIK_05880 0.35 +0.9 1,289,580 + phaC OKGIIK_05880 0.44 -1.8 1,289,580 + phaC OKGIIK_05880 0.44 -0.4 1,289,580 + phaC OKGIIK_05880 0.44 -1.2 1,289,581 - phaC OKGIIK_05880 0.44 +0.2 1,289,581 - phaC OKGIIK_05880 0.44 -0.8 1,289,739 + phaC OKGIIK_05880 0.59 -1.2 1,289,739 + phaC OKGIIK_05880 0.59 -0.7 1,289,739 + phaC OKGIIK_05880 0.59 -2.4 1,289,739 + phaC OKGIIK_05880 0.59 -2.0 1,289,739 + phaC OKGIIK_05880 0.59 +0.0 1,289,739 + phaC OKGIIK_05880 0.59 -1.4 1,289,739 + phaC OKGIIK_05880 0.59 +0.6 1,289,739 + phaC OKGIIK_05880 0.59 +0.6 1,289,739 + phaC OKGIIK_05880 0.59 +0.4 1,289,739 + phaC OKGIIK_05880 0.59 +0.0 1,289,740 - phaC OKGIIK_05880 0.59 -1.6 1,289,740 - phaC OKGIIK_05880 0.59 -0.2 1,289,740 - phaC OKGIIK_05880 0.59 -0.7 1,289,740 - phaC OKGIIK_05880 0.59 -0.7 1,289,740 - phaC OKGIIK_05880 0.59 -1.6 1,289,740 - phaC OKGIIK_05880 0.59 -1.0 1,289,740 - phaC OKGIIK_05880 0.59 -1.4 1,289,740 - phaC OKGIIK_05880 0.59 -1.6 1,289,740 - phaC OKGIIK_05880 0.59 -1.5 1,289,740 - phaC OKGIIK_05880 0.59 -1.4 1,289,784 + phaC OKGIIK_05880 0.63 -0.4 1,289,784 + phaC OKGIIK_05880 0.63 +0.6 1,289,784 + phaC OKGIIK_05880 0.63 -0.2 1,289,784 + phaC OKGIIK_05880 0.63 -1.7 1,289,784 + phaC OKGIIK_05880 0.63 -0.6 1,289,784 + phaC OKGIIK_05880 0.63 +1.0 1,289,785 - phaC OKGIIK_05880 0.63 +0.5 1,289,785 - phaC OKGIIK_05880 0.63 -0.6 1,289,785 - phaC OKGIIK_05880 0.63 -1.0 1,289,785 - phaC OKGIIK_05880 0.63 +0.6 1,289,785 - phaC OKGIIK_05880 0.63 -1.4 1,289,785 - phaC OKGIIK_05880 0.63 -1.6 1,289,785 - phaC OKGIIK_05880 0.63 -0.4 1,289,910 + phaC OKGIIK_05880 0.75 -0.3 1,289,910 + phaC OKGIIK_05880 0.75 +1.0 1,289,911 - phaC OKGIIK_05880 0.75 -1.5 1,289,911 - phaC OKGIIK_05880 0.75 -0.5 1,289,911 - phaC OKGIIK_05880 0.75 -0.2 1,289,911 - phaC OKGIIK_05880 0.75 -1.4 1,290,072 + phaC OKGIIK_05880 0.90 -1.5 1,290,072 + phaC OKGIIK_05880 0.90 -1.4 1,290,073 - +0.6 1,290,073 - -0.1 1,290,073 - +0.0 1,290,111 + +2.2 1,290,111 + -1.2 1,290,111 + -0.4 1,290,111 + -2.0 1,290,111 + -1.0 1,290,111 + +0.1 1,290,111 + -0.1 1,290,111 - -0.5 1,290,112 - +0.8 1,290,112 - -0.4 1,290,112 - +0.2 1,290,112 - -1.6 1,290,112 - -0.4 1,290,112 - -2.2 1,290,112 - -2.2 1,290,112 - +1.2 1,290,112 - -0.4 1,290,112 - -1.3 1,290,112 - -0.6 1,290,112 - +0.4 1,290,112 - +1.0 1,290,112 - -0.1 1,290,204 + +0.0 1,290,204 + -0.7 1,290,204 + -0.4 1,290,205 - -2.1 1,290,205 - +0.4 1,290,205 - -0.0 1,290,769 + mtnA OKGIIK_05885 0.53 -0.4 1,290,964 + mtnA OKGIIK_05885 0.72 -0.4 1,290,964 + mtnA OKGIIK_05885 0.72 -0.2 1,290,964 + mtnA OKGIIK_05885 0.72 -1.7 1,290,964 + mtnA OKGIIK_05885 0.72 +1.0 1,290,964 + mtnA OKGIIK_05885 0.72 +1.6 1,290,964 + mtnA OKGIIK_05885 0.72 -0.5 1,290,964 + mtnA OKGIIK_05885 0.72 -0.4 1,290,965 - mtnA OKGIIK_05885 0.72 -1.3 1,290,965 - mtnA OKGIIK_05885 0.72 -0.1 1,290,965 - mtnA OKGIIK_05885 0.72 -0.7 1,290,965 - mtnA OKGIIK_05885 0.72 -1.2 1,290,965 - mtnA OKGIIK_05885 0.72 -0.4 1,290,965 - mtnA OKGIIK_05885 0.72 +0.0 1,290,988 + mtnA OKGIIK_05885 0.74 -2.9 1,290,988 + mtnA OKGIIK_05885 0.74 +0.2 1,290,988 + mtnA OKGIIK_05885 0.74 -1.8 1,290,989 - mtnA OKGIIK_05885 0.74 -1.0 1,291,356 + OKGIIK_05890 0.27 +0.8 1,291,356 + OKGIIK_05890 0.27 -0.8 1,291,356 + OKGIIK_05890 0.27 -0.7 1,291,357 - OKGIIK_05890 0.27 +0.2 1,291,357 - OKGIIK_05890 0.27 -0.4 1,291,357 - OKGIIK_05890 0.27 +1.2 1,291,357 - OKGIIK_05890 0.27 +0.4 1,291,419 + OKGIIK_05890 0.42 -0.4 1,291,509 + OKGIIK_05890 0.64 -0.4 1,291,509 + OKGIIK_05890 0.64 +0.2 1,291,510 - OKGIIK_05890 0.64 -1.6 1,291,518 + OKGIIK_05890 0.66 -2.7 1,291,518 + OKGIIK_05890 0.66 +0.4 1,291,518 + OKGIIK_05890 0.66 +0.5 1,291,518 + OKGIIK_05890 0.66 -1.6 1,291,519 - OKGIIK_05890 0.66 +1.6 1,291,519 - OKGIIK_05890 0.66 +0.0 1,291,669 + -1.2 1,291,670 - +1.0 1,291,672 - +0.1 1,291,708 + -1.8 1,291,708 + +0.6 1,291,708 + +1.4 1,291,708 + -0.4 1,291,709 - -0.3 1,291,709 - -1.2 1,291,709 - +1.4 1,291,710 + -0.6 1,291,710 + +0.4 1,291,710 + +1.3 1,291,710 + -0.3 1,291,710 + -1.2 1,291,710 + -0.9 1,291,710 + -1.0 1,291,710 + +0.9 1,291,710 + +0.8 1,291,710 + -0.4 1,291,710 + +1.5 1,291,710 + +0.6 1,291,710 + -1.0 1,291,710 + +0.4
Or see this region's nucleotide sequence