Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_05205 and OKGIIK_05210 are separated by 158 nucleotides OKGIIK_05210 and OKGIIK_05215 are separated by 14 nucleotides OKGIIK_05215 and OKGIIK_05220 are separated by 617 nucleotides
OKGIIK_05205: OKGIIK_05205 - SseB protein, at 1,130,057 to 1,130,800
_05205
OKGIIK_05210: OKGIIK_05210 - HigA family addiction module antitoxin, at 1,130,959 to 1,131,243
_05210
OKGIIK_05215: OKGIIK_05215 - Killer protein, at 1,131,258 to 1,131,536
_05215
OKGIIK_05220: OKGIIK_05220 - Peptidase C39, at 1,132,154 to 1,134,337
_05220
Position (kb) 1130
1131
1132 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 1130.067 kb on + strand at 1130.067 kb on + strand at 1130.068 kb on - strand at 1130.068 kb on - strand at 1130.128 kb on + strand at 1130.128 kb on + strand at 1130.129 kb on - strand at 1130.129 kb on - strand at 1130.153 kb on + strand, within OKGIIK_05205 at 1130.153 kb on + strand, within OKGIIK_05205 at 1130.154 kb on - strand, within OKGIIK_05205 at 1130.154 kb on - strand, within OKGIIK_05205 at 1130.154 kb on - strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.180 kb on + strand, within OKGIIK_05205 at 1130.181 kb on + strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.181 kb on - strand, within OKGIIK_05205 at 1130.227 kb on + strand, within OKGIIK_05205 at 1130.228 kb on - strand, within OKGIIK_05205 at 1130.228 kb on - strand, within OKGIIK_05205 at 1130.228 kb on - strand, within OKGIIK_05205 at 1130.408 kb on + strand, within OKGIIK_05205 at 1130.409 kb on - strand, within OKGIIK_05205 at 1130.409 kb on - strand, within OKGIIK_05205 at 1130.458 kb on + strand, within OKGIIK_05205 at 1130.458 kb on + strand, within OKGIIK_05205 at 1130.458 kb on + strand, within OKGIIK_05205 at 1130.479 kb on + strand, within OKGIIK_05205 at 1130.547 kb on + strand, within OKGIIK_05205 at 1130.547 kb on + strand, within OKGIIK_05205 at 1130.547 kb on + strand, within OKGIIK_05205 at 1130.547 kb on + strand, within OKGIIK_05205 at 1130.547 kb on + strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.548 kb on - strand, within OKGIIK_05205 at 1130.550 kb on - strand, within OKGIIK_05205 at 1130.550 kb on - strand, within OKGIIK_05205 at 1130.550 kb on - strand, within OKGIIK_05205 at 1130.550 kb on - strand, within OKGIIK_05205 at 1130.550 kb on - strand, within OKGIIK_05205 at 1130.608 kb on + strand, within OKGIIK_05205 at 1130.609 kb on - strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.628 kb on + strand, within OKGIIK_05205 at 1130.629 kb on - strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.691 kb on + strand, within OKGIIK_05205 at 1130.692 kb on - strand, within OKGIIK_05205 at 1130.692 kb on - strand, within OKGIIK_05205 at 1130.692 kb on - strand, within OKGIIK_05205 at 1130.692 kb on - strand, within OKGIIK_05205 at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.766 kb on + strand at 1130.767 kb on - strand at 1130.767 kb on - strand at 1130.767 kb on - strand at 1130.767 kb on - strand at 1130.767 kb on - strand at 1130.790 kb on - strand at 1130.790 kb on - strand at 1130.803 kb on + strand at 1130.804 kb on - strand at 1130.804 kb on - strand at 1130.838 kb on + strand at 1130.838 kb on + strand at 1130.838 kb on + strand at 1130.838 kb on + strand at 1130.839 kb on - strand at 1130.839 kb on - strand at 1130.839 kb on - strand at 1130.839 kb on - strand at 1130.892 kb on + strand at 1130.903 kb on + strand at 1130.903 kb on + strand at 1130.903 kb on + strand at 1130.904 kb on - strand at 1130.924 kb on - strand at 1130.924 kb on - strand at 1130.957 kb on + strand at 1130.957 kb on + strand at 1130.958 kb on - strand at 1130.958 kb on - strand at 1130.961 kb on - strand at 1130.974 kb on + strand at 1130.975 kb on - strand at 1130.975 kb on - strand at 1130.975 kb on - strand at 1131.023 kb on + strand, within OKGIIK_05210 at 1131.023 kb on + strand, within OKGIIK_05210 at 1131.024 kb on - strand, within OKGIIK_05210 at 1131.024 kb on - strand, within OKGIIK_05210 at 1131.024 kb on - strand, within OKGIIK_05210 at 1131.024 kb on - strand, within OKGIIK_05210 at 1131.024 kb on - strand, within OKGIIK_05210 at 1131.089 kb on - strand, within OKGIIK_05210 at 1131.265 kb on + strand at 1131.265 kb on + strand at 1131.265 kb on + strand at 1131.265 kb on + strand at 1131.265 kb on + strand at 1131.265 kb on + strand at 1131.265 kb on + strand at 1131.266 kb on - strand at 1131.266 kb on - strand at 1131.266 kb on - strand at 1131.385 kb on + strand, within OKGIIK_05215 at 1131.385 kb on + strand, within OKGIIK_05215 at 1131.386 kb on - strand, within OKGIIK_05215 at 1131.528 kb on + strand at 1131.528 kb on + strand at 1131.528 kb on + strand at 1131.528 kb on + strand at 1131.529 kb on - strand at 1131.529 kb on - strand at 1131.529 kb on - strand at 1131.529 kb on - strand at 1131.537 kb on - strand at 1131.537 kb on - strand at 1131.537 kb on - strand at 1131.629 kb on - strand at 1131.694 kb on + strand at 1131.694 kb on + strand at 1131.694 kb on + strand at 1131.695 kb on - strand at 1131.695 kb on - strand at 1131.695 kb on - strand at 1131.695 kb on - strand at 1131.725 kb on + strand at 1131.725 kb on + strand at 1131.725 kb on + strand at 1131.725 kb on + strand at 1131.726 kb on - strand at 1131.726 kb on - strand at 1131.729 kb on + strand at 1131.729 kb on + strand at 1131.729 kb on + strand at 1131.729 kb on + strand at 1131.730 kb on - strand at 1131.730 kb on - strand at 1131.764 kb on + strand at 1131.862 kb on + strand at 1131.862 kb on + strand at 1131.863 kb on - strand at 1131.942 kb on + strand at 1131.942 kb on + strand at 1131.942 kb on + strand at 1131.942 kb on + strand at 1131.943 kb on - strand at 1131.943 kb on - strand at 1131.943 kb on - strand at 1131.943 kb on - strand at 1132.001 kb on - strand at 1132.027 kb on + strand at 1132.062 kb on + strand at 1132.062 kb on + strand at 1132.062 kb on + strand at 1132.063 kb on - strand at 1132.063 kb on - strand at 1132.063 kb on - strand at 1132.063 kb on - strand at 1132.063 kb on - strand at 1132.102 kb on + strand at 1132.102 kb on + strand at 1132.102 kb on + strand at 1132.102 kb on + strand at 1132.102 kb on + strand at 1132.102 kb on + strand at 1132.103 kb on - strand at 1132.125 kb on + strand at 1132.126 kb on - strand at 1132.134 kb on + strand at 1132.134 kb on + strand at 1132.134 kb on + strand at 1132.134 kb on + strand at 1132.135 kb on - strand at 1132.135 kb on - strand at 1132.167 kb on + strand at 1132.167 kb on + strand at 1132.167 kb on + strand at 1132.167 kb on + strand at 1132.168 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,130,067 + +1.6 1,130,067 + -0.6 1,130,068 - -1.0 1,130,068 - -0.5 1,130,128 + -0.8 1,130,128 + -1.2 1,130,129 - +0.9 1,130,129 - -0.1 1,130,153 + OKGIIK_05205 0.13 -0.6 1,130,153 + OKGIIK_05205 0.13 +0.6 1,130,154 - OKGIIK_05205 0.13 -0.7 1,130,154 - OKGIIK_05205 0.13 -1.0 1,130,154 - OKGIIK_05205 0.13 -0.5 1,130,180 + OKGIIK_05205 0.17 -1.0 1,130,180 + OKGIIK_05205 0.17 +0.2 1,130,180 + OKGIIK_05205 0.17 -0.4 1,130,180 + OKGIIK_05205 0.17 +0.2 1,130,180 + OKGIIK_05205 0.17 +0.8 1,130,180 + OKGIIK_05205 0.17 -1.4 1,130,180 + OKGIIK_05205 0.17 +0.4 1,130,180 + OKGIIK_05205 0.17 -0.4 1,130,180 + OKGIIK_05205 0.17 +0.9 1,130,180 + OKGIIK_05205 0.17 -2.2 1,130,180 + OKGIIK_05205 0.17 -0.7 1,130,180 + OKGIIK_05205 0.17 -0.2 1,130,180 + OKGIIK_05205 0.17 -0.8 1,130,180 + OKGIIK_05205 0.17 -1.6 1,130,181 + OKGIIK_05205 0.17 -0.5 1,130,181 - OKGIIK_05205 0.17 -0.7 1,130,181 - OKGIIK_05205 0.17 -0.7 1,130,181 - OKGIIK_05205 0.17 -0.2 1,130,181 - OKGIIK_05205 0.17 -0.3 1,130,181 - OKGIIK_05205 0.17 -1.2 1,130,181 - OKGIIK_05205 0.17 -1.0 1,130,181 - OKGIIK_05205 0.17 -1.7 1,130,181 - OKGIIK_05205 0.17 -0.7 1,130,181 - OKGIIK_05205 0.17 -0.1 1,130,181 - OKGIIK_05205 0.17 +0.4 1,130,181 - OKGIIK_05205 0.17 +0.0 1,130,227 + OKGIIK_05205 0.23 -0.6 1,130,228 - OKGIIK_05205 0.23 -3.0 1,130,228 - OKGIIK_05205 0.23 -0.4 1,130,228 - OKGIIK_05205 0.23 +0.3 1,130,408 + OKGIIK_05205 0.47 -1.6 1,130,409 - OKGIIK_05205 0.47 -1.4 1,130,409 - OKGIIK_05205 0.47 +0.8 1,130,458 + OKGIIK_05205 0.54 +0.0 1,130,458 + OKGIIK_05205 0.54 -2.7 1,130,458 + OKGIIK_05205 0.54 +0.3 1,130,479 + OKGIIK_05205 0.57 -0.2 1,130,547 + OKGIIK_05205 0.66 +0.0 1,130,547 + OKGIIK_05205 0.66 +0.2 1,130,547 + OKGIIK_05205 0.66 +1.4 1,130,547 + OKGIIK_05205 0.66 -0.6 1,130,547 + OKGIIK_05205 0.66 -1.9 1,130,548 - OKGIIK_05205 0.66 +0.1 1,130,548 - OKGIIK_05205 0.66 -1.8 1,130,548 - OKGIIK_05205 0.66 -1.6 1,130,548 - OKGIIK_05205 0.66 +0.3 1,130,548 - OKGIIK_05205 0.66 -2.2 1,130,548 - OKGIIK_05205 0.66 -0.7 1,130,548 - OKGIIK_05205 0.66 -0.7 1,130,548 - OKGIIK_05205 0.66 -1.6 1,130,548 - OKGIIK_05205 0.66 -0.1 1,130,548 - OKGIIK_05205 0.66 -0.6 1,130,550 - OKGIIK_05205 0.66 +0.1 1,130,550 - OKGIIK_05205 0.66 -0.8 1,130,550 - OKGIIK_05205 0.66 -1.9 1,130,550 - OKGIIK_05205 0.66 -0.4 1,130,550 - OKGIIK_05205 0.66 -1.2 1,130,608 + OKGIIK_05205 0.74 -2.7 1,130,609 - OKGIIK_05205 0.74 -1.8 1,130,628 + OKGIIK_05205 0.77 +0.6 1,130,628 + OKGIIK_05205 0.77 -0.5 1,130,628 + OKGIIK_05205 0.77 -0.5 1,130,628 + OKGIIK_05205 0.77 +0.3 1,130,628 + OKGIIK_05205 0.77 +0.0 1,130,628 + OKGIIK_05205 0.77 -0.6 1,130,628 + OKGIIK_05205 0.77 -0.8 1,130,628 + OKGIIK_05205 0.77 -0.1 1,130,629 - OKGIIK_05205 0.77 -1.7 1,130,691 + OKGIIK_05205 0.85 -0.2 1,130,691 + OKGIIK_05205 0.85 -0.4 1,130,691 + OKGIIK_05205 0.85 +0.8 1,130,691 + OKGIIK_05205 0.85 +0.3 1,130,691 + OKGIIK_05205 0.85 -1.2 1,130,691 + OKGIIK_05205 0.85 -0.7 1,130,691 + OKGIIK_05205 0.85 +1.2 1,130,691 + OKGIIK_05205 0.85 -0.3 1,130,691 + OKGIIK_05205 0.85 +0.6 1,130,692 - OKGIIK_05205 0.85 -0.1 1,130,692 - OKGIIK_05205 0.85 -1.5 1,130,692 - OKGIIK_05205 0.85 -0.3 1,130,692 - OKGIIK_05205 0.85 +1.2 1,130,766 + -1.6 1,130,766 + -0.4 1,130,766 + -2.3 1,130,766 + +0.3 1,130,766 + -0.6 1,130,766 + -0.4 1,130,766 + +0.6 1,130,766 + +0.6 1,130,766 + -0.1 1,130,766 + -0.4 1,130,766 + +0.3 1,130,767 - +0.9 1,130,767 - +0.0 1,130,767 - +0.9 1,130,767 - -1.6 1,130,767 - -1.2 1,130,790 - -0.1 1,130,790 - -0.9 1,130,803 + +0.9 1,130,804 - +1.8 1,130,804 - +0.5 1,130,838 + -2.0 1,130,838 + +1.3 1,130,838 + -2.8 1,130,838 + -0.7 1,130,839 - +1.1 1,130,839 - -2.5 1,130,839 - +0.7 1,130,839 - -0.7 1,130,892 + +1.9 1,130,903 + -0.6 1,130,903 + -1.0 1,130,903 + -1.8 1,130,904 - -1.4 1,130,924 - -2.1 1,130,924 - +0.9 1,130,957 + +0.9 1,130,957 + -0.5 1,130,958 - -0.7 1,130,958 - +1.4 1,130,961 - +0.3 1,130,974 + -1.6 1,130,975 - +0.6 1,130,975 - +0.7 1,130,975 - -0.6 1,131,023 + OKGIIK_05210 0.22 +0.6 1,131,023 + OKGIIK_05210 0.22 -1.0 1,131,024 - OKGIIK_05210 0.23 +0.3 1,131,024 - OKGIIK_05210 0.23 -0.7 1,131,024 - OKGIIK_05210 0.23 -2.4 1,131,024 - OKGIIK_05210 0.23 -1.7 1,131,024 - OKGIIK_05210 0.23 -1.1 1,131,089 - OKGIIK_05210 0.46 -2.0 1,131,265 + -0.3 1,131,265 + -0.7 1,131,265 + -0.9 1,131,265 + -0.7 1,131,265 + +0.6 1,131,265 + -1.7 1,131,265 + +1.9 1,131,266 - -0.1 1,131,266 - -0.7 1,131,266 - -0.2 1,131,385 + OKGIIK_05215 0.46 -1.2 1,131,385 + OKGIIK_05215 0.46 +0.9 1,131,386 - OKGIIK_05215 0.46 -1.0 1,131,528 + -0.9 1,131,528 + -1.3 1,131,528 + -1.2 1,131,528 + -0.7 1,131,529 - -1.7 1,131,529 - -2.6 1,131,529 - +1.0 1,131,529 - -0.7 1,131,537 - -0.6 1,131,537 - +0.8 1,131,537 - +0.3 1,131,629 - -0.2 1,131,694 + -0.2 1,131,694 + +0.3 1,131,694 + +1.9 1,131,695 - -0.6 1,131,695 - +0.0 1,131,695 - -1.0 1,131,695 - +0.5 1,131,725 + +0.2 1,131,725 + -1.0 1,131,725 + +1.1 1,131,725 + -0.7 1,131,726 - -0.2 1,131,726 - -0.1 1,131,729 + -2.2 1,131,729 + -0.2 1,131,729 + -1.0 1,131,729 + +0.3 1,131,730 - -0.1 1,131,730 - -0.0 1,131,764 + +0.1 1,131,862 + -1.4 1,131,862 + -0.8 1,131,863 - +0.6 1,131,942 + -0.4 1,131,942 + -0.2 1,131,942 + -0.3 1,131,942 + +0.1 1,131,943 - -0.4 1,131,943 - +0.4 1,131,943 - +0.1 1,131,943 - -0.4 1,132,001 - +0.0 1,132,027 + -0.9 1,132,062 + +0.3 1,132,062 + +0.3 1,132,062 + -0.4 1,132,063 - +0.0 1,132,063 - -0.1 1,132,063 - -0.6 1,132,063 - -1.0 1,132,063 - -2.3 1,132,102 + -1.0 1,132,102 + -0.9 1,132,102 + -2.2 1,132,102 + -1.0 1,132,102 + +0.0 1,132,102 + -0.7 1,132,103 - +1.2 1,132,125 + +0.7 1,132,126 - +0.4 1,132,134 + +1.2 1,132,134 + +0.1 1,132,134 + -1.0 1,132,134 + -0.4 1,132,135 - -0.6 1,132,135 - +0.7 1,132,167 + -2.0 1,132,167 + +0.5 1,132,167 + -2.0 1,132,167 + +0.8 1,132,168 - -1.6
Or see this region's nucleotide sequence