Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisH and hisA overlap by 4 nucleotides hisA and hisF are separated by 88 nucleotides hisF and hisIE are separated by 104 nucleotides hisIE and OKGIIK_04985 are separated by 6 nucleotides
OKGIIK_04965: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,077,816 to 1,078,403
hisH
OKGIIK_04970: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 1,078,400 to 1,079,128
hisA
OKGIIK_04975: hisF - imidazole glycerol phosphate synthase subunit HisF, at 1,079,217 to 1,079,990
hisF
OKGIIK_04980: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,080,095 to 1,080,721
hisIE
OKGIIK_04985: OKGIIK_04985 - peptidase C13, at 1,080,728 to 1,081,738
_04985
Position (kb)
1079
1080 Strain fitness (log2 ratio)
-2
-1
0
1 at 1078.230 kb on + strand, within hisH at 1078.230 kb on + strand, within hisH at 1078.230 kb on + strand, within hisH at 1078.230 kb on + strand, within hisH at 1078.231 kb on - strand, within hisH at 1078.231 kb on - strand, within hisH at 1078.231 kb on - strand, within hisH at 1078.245 kb on + strand, within hisH at 1078.245 kb on + strand, within hisH at 1078.245 kb on + strand, within hisH at 1078.245 kb on + strand, within hisH at 1078.246 kb on - strand, within hisH at 1078.246 kb on - strand, within hisH at 1078.246 kb on - strand, within hisH at 1078.246 kb on - strand, within hisH at 1078.246 kb on - strand, within hisH at 1078.399 kb on + strand at 1078.399 kb on + strand at 1078.399 kb on + strand at 1078.399 kb on + strand at 1078.400 kb on - strand at 1078.400 kb on - strand at 1078.400 kb on - strand at 1078.400 kb on - strand at 1078.400 kb on - strand at 1078.470 kb on - strand at 1078.470 kb on - strand at 1078.487 kb on + strand, within hisA at 1078.487 kb on + strand, within hisA at 1078.487 kb on + strand, within hisA at 1078.487 kb on + strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.488 kb on - strand, within hisA at 1078.521 kb on - strand, within hisA at 1078.651 kb on - strand, within hisA at 1078.651 kb on - strand, within hisA at 1078.760 kb on + strand, within hisA at 1078.880 kb on + strand, within hisA at 1078.881 kb on - strand, within hisA at 1078.881 kb on - strand, within hisA at 1078.881 kb on - strand, within hisA at 1078.962 kb on - strand, within hisA at 1079.138 kb on + strand at 1079.138 kb on + strand at 1079.138 kb on + strand at 1079.138 kb on + strand at 1079.139 kb on - strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.216 kb on + strand at 1079.217 kb on - strand at 1079.364 kb on + strand, within hisF at 1079.364 kb on + strand, within hisF at 1079.364 kb on + strand, within hisF at 1079.364 kb on + strand, within hisF at 1079.364 kb on + strand, within hisF at 1079.365 kb on - strand, within hisF at 1079.365 kb on - strand, within hisF at 1079.365 kb on - strand, within hisF at 1079.365 kb on - strand, within hisF at 1079.612 kb on - strand, within hisF at 1079.646 kb on + strand, within hisF at 1079.646 kb on + strand, within hisF at 1079.647 kb on - strand, within hisF at 1079.647 kb on - strand, within hisF at 1079.952 kb on + strand at 1079.952 kb on + strand at 1079.952 kb on + strand at 1079.971 kb on + strand at 1079.971 kb on + strand at 1079.971 kb on + strand at 1079.971 kb on + strand at 1079.972 kb on - strand at 1079.972 kb on - strand at 1079.972 kb on - strand at 1079.972 kb on - strand at 1079.972 kb on - strand at 1079.972 kb on - strand at 1079.972 kb on - strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.058 kb on + strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.059 kb on - strand at 1080.203 kb on + strand, within hisIE at 1080.203 kb on + strand, within hisIE at 1080.203 kb on + strand, within hisIE at 1080.203 kb on + strand, within hisIE at 1080.204 kb on - strand, within hisIE at 1080.204 kb on - strand, within hisIE at 1080.204 kb on - strand, within hisIE at 1080.204 kb on - strand, within hisIE at 1080.204 kb on - strand, within hisIE at 1080.204 kb on - strand, within hisIE at 1080.257 kb on + strand, within hisIE at 1080.257 kb on + strand, within hisIE at 1080.257 kb on + strand, within hisIE at 1080.257 kb on + strand, within hisIE at 1080.258 kb on - strand, within hisIE at 1080.258 kb on - strand, within hisIE at 1080.258 kb on - strand, within hisIE at 1080.349 kb on + strand, within hisIE at 1080.349 kb on + strand, within hisIE at 1080.349 kb on + strand, within hisIE at 1080.350 kb on - strand, within hisIE at 1080.350 kb on - strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.500 kb on + strand, within hisIE at 1080.501 kb on - strand, within hisIE at 1080.501 kb on - strand, within hisIE at 1080.501 kb on - strand, within hisIE at 1080.501 kb on - strand, within hisIE at 1080.501 kb on - strand, within hisIE at 1080.981 kb on + strand, within OKGIIK_04985 at 1080.981 kb on + strand, within OKGIIK_04985 at 1080.981 kb on + strand, within OKGIIK_04985 at 1080.981 kb on + strand, within OKGIIK_04985 at 1080.981 kb on + strand, within OKGIIK_04985 at 1080.981 kb on + strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985 at 1080.982 kb on - strand, within OKGIIK_04985
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,078,230 + hisH OKGIIK_04965 0.70 -0.9 1,078,230 + hisH OKGIIK_04965 0.70 +1.3 1,078,230 + hisH OKGIIK_04965 0.70 -1.0 1,078,230 + hisH OKGIIK_04965 0.70 +0.4 1,078,231 - hisH OKGIIK_04965 0.71 -0.7 1,078,231 - hisH OKGIIK_04965 0.71 -2.0 1,078,231 - hisH OKGIIK_04965 0.71 -1.6 1,078,245 + hisH OKGIIK_04965 0.73 -1.2 1,078,245 + hisH OKGIIK_04965 0.73 -0.5 1,078,245 + hisH OKGIIK_04965 0.73 -1.2 1,078,245 + hisH OKGIIK_04965 0.73 -0.6 1,078,246 - hisH OKGIIK_04965 0.73 -0.7 1,078,246 - hisH OKGIIK_04965 0.73 -1.0 1,078,246 - hisH OKGIIK_04965 0.73 +0.6 1,078,246 - hisH OKGIIK_04965 0.73 -1.0 1,078,246 - hisH OKGIIK_04965 0.73 +1.4 1,078,399 + -0.6 1,078,399 + -1.9 1,078,399 + +1.6 1,078,399 + +0.4 1,078,400 - +1.2 1,078,400 - +1.0 1,078,400 - +1.3 1,078,400 - -0.5 1,078,400 - -0.6 1,078,470 - -2.3 1,078,470 - -0.7 1,078,487 + hisA OKGIIK_04970 0.12 -0.7 1,078,487 + hisA OKGIIK_04970 0.12 -1.2 1,078,487 + hisA OKGIIK_04970 0.12 -1.0 1,078,487 + hisA OKGIIK_04970 0.12 -0.7 1,078,488 - hisA OKGIIK_04970 0.12 -1.4 1,078,488 - hisA OKGIIK_04970 0.12 -0.4 1,078,488 - hisA OKGIIK_04970 0.12 +0.6 1,078,488 - hisA OKGIIK_04970 0.12 -1.7 1,078,488 - hisA OKGIIK_04970 0.12 -1.9 1,078,488 - hisA OKGIIK_04970 0.12 -1.6 1,078,488 - hisA OKGIIK_04970 0.12 -2.0 1,078,488 - hisA OKGIIK_04970 0.12 -0.4 1,078,488 - hisA OKGIIK_04970 0.12 +0.4 1,078,488 - hisA OKGIIK_04970 0.12 -0.9 1,078,521 - hisA OKGIIK_04970 0.17 -1.5 1,078,651 - hisA OKGIIK_04970 0.34 +0.6 1,078,651 - hisA OKGIIK_04970 0.34 -0.4 1,078,760 + hisA OKGIIK_04970 0.49 -1.2 1,078,880 + hisA OKGIIK_04970 0.66 -0.7 1,078,881 - hisA OKGIIK_04970 0.66 -1.2 1,078,881 - hisA OKGIIK_04970 0.66 +0.6 1,078,881 - hisA OKGIIK_04970 0.66 -0.4 1,078,962 - hisA OKGIIK_04970 0.77 -2.1 1,079,138 + -1.0 1,079,138 + -0.4 1,079,138 + -0.0 1,079,138 + +0.3 1,079,139 - -0.9 1,079,216 + +1.2 1,079,216 + -0.7 1,079,216 + -0.4 1,079,216 + -0.3 1,079,216 + -1.2 1,079,216 + -0.7 1,079,216 + -0.4 1,079,216 + +0.2 1,079,216 + +0.2 1,079,217 - -0.0 1,079,364 + hisF OKGIIK_04975 0.19 -2.0 1,079,364 + hisF OKGIIK_04975 0.19 -1.9 1,079,364 + hisF OKGIIK_04975 0.19 -0.7 1,079,364 + hisF OKGIIK_04975 0.19 -1.6 1,079,364 + hisF OKGIIK_04975 0.19 -0.3 1,079,365 - hisF OKGIIK_04975 0.19 -1.1 1,079,365 - hisF OKGIIK_04975 0.19 -1.0 1,079,365 - hisF OKGIIK_04975 0.19 -0.2 1,079,365 - hisF OKGIIK_04975 0.19 -0.7 1,079,612 - hisF OKGIIK_04975 0.51 -1.0 1,079,646 + hisF OKGIIK_04975 0.55 -0.4 1,079,646 + hisF OKGIIK_04975 0.55 +0.8 1,079,647 - hisF OKGIIK_04975 0.56 -1.1 1,079,647 - hisF OKGIIK_04975 0.56 -0.4 1,079,952 + -1.0 1,079,952 + -0.7 1,079,952 + -0.7 1,079,971 + -0.7 1,079,971 + -2.7 1,079,971 + -0.3 1,079,971 + +0.7 1,079,972 - -0.5 1,079,972 - +0.4 1,079,972 - -1.1 1,079,972 - -1.4 1,079,972 - -0.4 1,079,972 - +0.3 1,079,972 - -0.1 1,080,058 + +1.2 1,080,058 + -0.7 1,080,058 + -1.2 1,080,058 + -1.2 1,080,058 + -0.4 1,080,058 + -2.2 1,080,058 + -0.4 1,080,058 + -1.2 1,080,058 + -0.4 1,080,058 + +0.4 1,080,058 + +0.8 1,080,058 + +0.5 1,080,058 + -1.3 1,080,058 + -0.4 1,080,059 - -1.7 1,080,059 - -1.4 1,080,059 - -1.7 1,080,059 - -1.9 1,080,059 - +1.2 1,080,059 - +1.3 1,080,059 - -2.3 1,080,059 - +0.1 1,080,059 - -1.4 1,080,059 - -0.7 1,080,059 - +0.0 1,080,059 - +0.0 1,080,059 - +1.6 1,080,059 - +0.3 1,080,203 + hisIE OKGIIK_04980 0.17 -0.6 1,080,203 + hisIE OKGIIK_04980 0.17 -1.2 1,080,203 + hisIE OKGIIK_04980 0.17 -1.6 1,080,203 + hisIE OKGIIK_04980 0.17 +0.6 1,080,204 - hisIE OKGIIK_04980 0.17 -1.6 1,080,204 - hisIE OKGIIK_04980 0.17 -0.1 1,080,204 - hisIE OKGIIK_04980 0.17 +0.3 1,080,204 - hisIE OKGIIK_04980 0.17 +1.3 1,080,204 - hisIE OKGIIK_04980 0.17 -0.7 1,080,204 - hisIE OKGIIK_04980 0.17 -0.7 1,080,257 + hisIE OKGIIK_04980 0.26 -0.9 1,080,257 + hisIE OKGIIK_04980 0.26 -1.4 1,080,257 + hisIE OKGIIK_04980 0.26 +0.3 1,080,257 + hisIE OKGIIK_04980 0.26 -0.5 1,080,258 - hisIE OKGIIK_04980 0.26 +0.8 1,080,258 - hisIE OKGIIK_04980 0.26 +0.3 1,080,258 - hisIE OKGIIK_04980 0.26 -1.6 1,080,349 + hisIE OKGIIK_04980 0.41 -0.5 1,080,349 + hisIE OKGIIK_04980 0.41 -1.0 1,080,349 + hisIE OKGIIK_04980 0.41 -0.7 1,080,350 - hisIE OKGIIK_04980 0.41 -1.5 1,080,350 - hisIE OKGIIK_04980 0.41 -0.5 1,080,500 + hisIE OKGIIK_04980 0.65 +0.3 1,080,500 + hisIE OKGIIK_04980 0.65 -1.5 1,080,500 + hisIE OKGIIK_04980 0.65 -0.5 1,080,500 + hisIE OKGIIK_04980 0.65 -0.6 1,080,500 + hisIE OKGIIK_04980 0.65 -0.9 1,080,500 + hisIE OKGIIK_04980 0.65 -0.2 1,080,500 + hisIE OKGIIK_04980 0.65 -1.4 1,080,500 + hisIE OKGIIK_04980 0.65 -2.3 1,080,501 - hisIE OKGIIK_04980 0.65 -0.4 1,080,501 - hisIE OKGIIK_04980 0.65 +1.0 1,080,501 - hisIE OKGIIK_04980 0.65 +0.4 1,080,501 - hisIE OKGIIK_04980 0.65 -1.2 1,080,501 - hisIE OKGIIK_04980 0.65 -0.9 1,080,981 + OKGIIK_04985 0.25 -0.6 1,080,981 + OKGIIK_04985 0.25 +0.6 1,080,981 + OKGIIK_04985 0.25 +0.8 1,080,981 + OKGIIK_04985 0.25 +0.0 1,080,981 + OKGIIK_04985 0.25 -2.3 1,080,981 + OKGIIK_04985 0.25 +0.9 1,080,982 - OKGIIK_04985 0.25 +0.3 1,080,982 - OKGIIK_04985 0.25 -0.4 1,080,982 - OKGIIK_04985 0.25 -0.6 1,080,982 - OKGIIK_04985 0.25 -0.7 1,080,982 - OKGIIK_04985 0.25 -1.4 1,080,982 - OKGIIK_04985 0.25 -0.3 1,080,982 - OKGIIK_04985 0.25 -0.2 1,080,982 - OKGIIK_04985 0.25 -0.6 1,080,982 - OKGIIK_04985 0.25 -1.6 1,080,982 - OKGIIK_04985 0.25 -1.6
Or see this region's nucleotide sequence