Strain Fitness in Rhodanobacter sp. FW510-T8 around OKGIIK_04970

Experiment: R2A_PIPES with Nickel (II) chloride 424 uM

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 nthisB and hisH are separated by 112 nucleotideshisH and hisA overlap by 4 nucleotideshisA and hisF are separated by 88 nucleotideshisF and hisIE are separated by 104 nucleotides OKGIIK_04960: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,076,636 to 1,077,703 hisB OKGIIK_04965: hisH - imidazole glycerol phosphate synthase subunit HisH, at 1,077,816 to 1,078,403 hisH OKGIIK_04970: hisA - 1-(5-phosphoribosyl)-5-[(5-phosphoribosylamino)me thylideneamino]imidazole-4-carboxamide isomerase, at 1,078,400 to 1,079,128 hisA OKGIIK_04975: hisF - imidazole glycerol phosphate synthase subunit HisF, at 1,079,217 to 1,079,990 hisF OKGIIK_04980: hisIE - bifunctional phosphoribosyl-AMP cyclohydrolase/phosphoribosyl-ATP diphosphatase HisIE, at 1,080,095 to 1,080,721 hisIE Position (kb) 1078 1079 1080Strain fitness (log2 ratio) -3 -2 -1 0 1 2at 1077.467 kb on + strand, within hisBat 1077.467 kb on + strand, within hisBat 1077.467 kb on + strand, within hisBat 1077.467 kb on + strand, within hisBat 1077.468 kb on - strand, within hisBat 1077.468 kb on - strand, within hisBat 1077.468 kb on - strand, within hisBat 1077.504 kb on + strand, within hisBat 1077.505 kb on - strand, within hisBat 1077.701 kb on + strandat 1077.701 kb on + strandat 1077.701 kb on + strandat 1077.701 kb on + strandat 1077.701 kb on + strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.702 kb on - strandat 1077.750 kb on + strandat 1077.751 kb on - strandat 1077.751 kb on - strandat 1077.808 kb on + strandat 1077.808 kb on + strandat 1077.809 kb on - strandat 1077.809 kb on - strandat 1077.809 kb on - strandat 1077.809 kb on - strandat 1077.809 kb on - strandat 1077.809 kb on - strandat 1078.157 kb on + strand, within hisHat 1078.157 kb on + strand, within hisHat 1078.157 kb on + strand, within hisHat 1078.158 kb on - strand, within hisHat 1078.158 kb on - strand, within hisHat 1078.230 kb on + strand, within hisHat 1078.230 kb on + strand, within hisHat 1078.230 kb on + strand, within hisHat 1078.230 kb on + strand, within hisHat 1078.231 kb on - strand, within hisHat 1078.231 kb on - strand, within hisHat 1078.231 kb on - strand, within hisHat 1078.245 kb on + strand, within hisHat 1078.245 kb on + strand, within hisHat 1078.245 kb on + strand, within hisHat 1078.245 kb on + strand, within hisHat 1078.246 kb on - strand, within hisHat 1078.246 kb on - strand, within hisHat 1078.246 kb on - strand, within hisHat 1078.246 kb on - strand, within hisHat 1078.246 kb on - strand, within hisHat 1078.399 kb on + strandat 1078.399 kb on + strandat 1078.399 kb on + strandat 1078.399 kb on + strandat 1078.400 kb on - strandat 1078.400 kb on - strandat 1078.400 kb on - strandat 1078.400 kb on - strandat 1078.400 kb on - strandat 1078.470 kb on - strandat 1078.470 kb on - strandat 1078.487 kb on + strand, within hisAat 1078.487 kb on + strand, within hisAat 1078.487 kb on + strand, within hisAat 1078.487 kb on + strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.488 kb on - strand, within hisAat 1078.521 kb on - strand, within hisAat 1078.651 kb on - strand, within hisAat 1078.651 kb on - strand, within hisAat 1078.760 kb on + strand, within hisAat 1078.880 kb on + strand, within hisAat 1078.881 kb on - strand, within hisAat 1078.881 kb on - strand, within hisAat 1078.881 kb on - strand, within hisAat 1078.962 kb on - strand, within hisAat 1079.138 kb on + strandat 1079.138 kb on + strandat 1079.138 kb on + strandat 1079.138 kb on + strandat 1079.139 kb on - strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.216 kb on + strandat 1079.217 kb on - strandat 1079.364 kb on + strand, within hisFat 1079.364 kb on + strand, within hisFat 1079.364 kb on + strand, within hisFat 1079.364 kb on + strand, within hisFat 1079.364 kb on + strand, within hisFat 1079.365 kb on - strand, within hisFat 1079.365 kb on - strand, within hisFat 1079.365 kb on - strand, within hisFat 1079.365 kb on - strand, within hisFat 1079.612 kb on - strand, within hisFat 1079.646 kb on + strand, within hisFat 1079.646 kb on + strand, within hisFat 1079.647 kb on - strand, within hisFat 1079.647 kb on - strand, within hisFat 1079.952 kb on + strandat 1079.952 kb on + strandat 1079.952 kb on + strandat 1079.971 kb on + strandat 1079.971 kb on + strandat 1079.971 kb on + strandat 1079.971 kb on + strandat 1079.972 kb on - strandat 1079.972 kb on - strandat 1079.972 kb on - strandat 1079.972 kb on - strandat 1079.972 kb on - strandat 1079.972 kb on - strandat 1079.972 kb on - strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.058 kb on + strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strandat 1080.059 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM
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1,077,467 + hisB OKGIIK_04960 0.78 -0.7
1,077,467 + hisB OKGIIK_04960 0.78 +0.3
1,077,467 + hisB OKGIIK_04960 0.78 -0.7
1,077,467 + hisB OKGIIK_04960 0.78 -0.6
1,077,468 - hisB OKGIIK_04960 0.78 -2.4
1,077,468 - hisB OKGIIK_04960 0.78 +0.3
1,077,468 - hisB OKGIIK_04960 0.78 -1.0
1,077,504 + hisB OKGIIK_04960 0.81 -2.9
1,077,505 - hisB OKGIIK_04960 0.81 -1.2
1,077,701 + +1.9
1,077,701 + -0.0
1,077,701 + -0.7
1,077,701 + -0.0
1,077,701 + +1.1
1,077,702 - +0.8
1,077,702 - -0.7
1,077,702 - +1.0
1,077,702 - +0.6
1,077,702 - -1.2
1,077,702 - -1.2
1,077,702 - +0.1
1,077,702 - -0.4
1,077,702 - -0.6
1,077,702 - +0.6
1,077,702 - -0.2
1,077,750 + -1.3
1,077,751 - -0.7
1,077,751 - -0.3
1,077,808 + -1.4
1,077,808 + -1.3
1,077,809 - -1.4
1,077,809 - -1.0
1,077,809 - -1.4
1,077,809 - -0.6
1,077,809 - -0.4
1,077,809 - -1.0
1,078,157 + hisH OKGIIK_04965 0.58 -0.4
1,078,157 + hisH OKGIIK_04965 0.58 -0.5
1,078,157 + hisH OKGIIK_04965 0.58 +0.3
1,078,158 - hisH OKGIIK_04965 0.58 -0.4
1,078,158 - hisH OKGIIK_04965 0.58 -0.7
1,078,230 + hisH OKGIIK_04965 0.70 -0.9
1,078,230 + hisH OKGIIK_04965 0.70 +1.3
1,078,230 + hisH OKGIIK_04965 0.70 -1.0
1,078,230 + hisH OKGIIK_04965 0.70 +0.4
1,078,231 - hisH OKGIIK_04965 0.71 -0.7
1,078,231 - hisH OKGIIK_04965 0.71 -2.0
1,078,231 - hisH OKGIIK_04965 0.71 -1.6
1,078,245 + hisH OKGIIK_04965 0.73 -1.2
1,078,245 + hisH OKGIIK_04965 0.73 -0.5
1,078,245 + hisH OKGIIK_04965 0.73 -1.2
1,078,245 + hisH OKGIIK_04965 0.73 -0.6
1,078,246 - hisH OKGIIK_04965 0.73 -0.7
1,078,246 - hisH OKGIIK_04965 0.73 -1.0
1,078,246 - hisH OKGIIK_04965 0.73 +0.6
1,078,246 - hisH OKGIIK_04965 0.73 -1.0
1,078,246 - hisH OKGIIK_04965 0.73 +1.4
1,078,399 + -0.6
1,078,399 + -1.9
1,078,399 + +1.6
1,078,399 + +0.4
1,078,400 - +1.2
1,078,400 - +1.0
1,078,400 - +1.3
1,078,400 - -0.5
1,078,400 - -0.6
1,078,470 - -2.3
1,078,470 - -0.7
1,078,487 + hisA OKGIIK_04970 0.12 -0.7
1,078,487 + hisA OKGIIK_04970 0.12 -1.2
1,078,487 + hisA OKGIIK_04970 0.12 -1.0
1,078,487 + hisA OKGIIK_04970 0.12 -0.7
1,078,488 - hisA OKGIIK_04970 0.12 -1.4
1,078,488 - hisA OKGIIK_04970 0.12 -0.4
1,078,488 - hisA OKGIIK_04970 0.12 +0.6
1,078,488 - hisA OKGIIK_04970 0.12 -1.7
1,078,488 - hisA OKGIIK_04970 0.12 -1.9
1,078,488 - hisA OKGIIK_04970 0.12 -1.6
1,078,488 - hisA OKGIIK_04970 0.12 -2.0
1,078,488 - hisA OKGIIK_04970 0.12 -0.4
1,078,488 - hisA OKGIIK_04970 0.12 +0.4
1,078,488 - hisA OKGIIK_04970 0.12 -0.9
1,078,521 - hisA OKGIIK_04970 0.17 -1.5
1,078,651 - hisA OKGIIK_04970 0.34 +0.6
1,078,651 - hisA OKGIIK_04970 0.34 -0.4
1,078,760 + hisA OKGIIK_04970 0.49 -1.2
1,078,880 + hisA OKGIIK_04970 0.66 -0.7
1,078,881 - hisA OKGIIK_04970 0.66 -1.2
1,078,881 - hisA OKGIIK_04970 0.66 +0.6
1,078,881 - hisA OKGIIK_04970 0.66 -0.4
1,078,962 - hisA OKGIIK_04970 0.77 -2.1
1,079,138 + -1.0
1,079,138 + -0.4
1,079,138 + -0.0
1,079,138 + +0.3
1,079,139 - -0.9
1,079,216 + +1.2
1,079,216 + -0.7
1,079,216 + -0.4
1,079,216 + -0.3
1,079,216 + -1.2
1,079,216 + -0.7
1,079,216 + -0.4
1,079,216 + +0.2
1,079,216 + +0.2
1,079,217 - -0.0
1,079,364 + hisF OKGIIK_04975 0.19 -2.0
1,079,364 + hisF OKGIIK_04975 0.19 -1.9
1,079,364 + hisF OKGIIK_04975 0.19 -0.7
1,079,364 + hisF OKGIIK_04975 0.19 -1.6
1,079,364 + hisF OKGIIK_04975 0.19 -0.3
1,079,365 - hisF OKGIIK_04975 0.19 -1.1
1,079,365 - hisF OKGIIK_04975 0.19 -1.0
1,079,365 - hisF OKGIIK_04975 0.19 -0.2
1,079,365 - hisF OKGIIK_04975 0.19 -0.7
1,079,612 - hisF OKGIIK_04975 0.51 -1.0
1,079,646 + hisF OKGIIK_04975 0.55 -0.4
1,079,646 + hisF OKGIIK_04975 0.55 +0.8
1,079,647 - hisF OKGIIK_04975 0.56 -1.1
1,079,647 - hisF OKGIIK_04975 0.56 -0.4
1,079,952 + -1.0
1,079,952 + -0.7
1,079,952 + -0.7
1,079,971 + -0.7
1,079,971 + -2.7
1,079,971 + -0.3
1,079,971 + +0.7
1,079,972 - -0.5
1,079,972 - +0.4
1,079,972 - -1.1
1,079,972 - -1.4
1,079,972 - -0.4
1,079,972 - +0.3
1,079,972 - -0.1
1,080,058 + +1.2
1,080,058 + -0.7
1,080,058 + -1.2
1,080,058 + -1.2
1,080,058 + -0.4
1,080,058 + -2.2
1,080,058 + -0.4
1,080,058 + -1.2
1,080,058 + -0.4
1,080,058 + +0.4
1,080,058 + +0.8
1,080,058 + +0.5
1,080,058 + -1.3
1,080,058 + -0.4
1,080,059 - -1.7
1,080,059 - -1.4
1,080,059 - -1.7
1,080,059 - -1.9
1,080,059 - +1.2
1,080,059 - +1.3
1,080,059 - -2.3
1,080,059 - +0.1
1,080,059 - -1.4
1,080,059 - -0.7
1,080,059 - +0.0
1,080,059 - +0.0
1,080,059 - +1.6
1,080,059 - +0.3

Or see this region's nucleotide sequence