Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hisD and hisC overlap by 4 nucleotides hisC and hisB overlap by 4 nucleotides
OKGIIK_04950: hisD - histidinol dehydrogenase, at 1,074,253 to 1,075,551
hisD
OKGIIK_04955: hisC - histidinol-phosphate transaminase, at 1,075,548 to 1,076,639
hisC
OKGIIK_04960: hisB - bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB, at 1,076,636 to 1,077,703
hisB
Position (kb)
1075
1076
1077 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.631 kb on + strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.632 kb on - strand, within hisD at 1074.766 kb on + strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.767 kb on - strand, within hisD at 1074.838 kb on + strand, within hisD at 1074.839 kb on - strand, within hisD at 1075.336 kb on + strand, within hisD at 1075.336 kb on + strand, within hisD at 1075.336 kb on + strand, within hisD at 1075.336 kb on + strand, within hisD at 1075.337 kb on - strand, within hisD at 1075.337 kb on - strand, within hisD at 1075.369 kb on + strand, within hisD at 1075.369 kb on + strand, within hisD at 1075.369 kb on + strand, within hisD at 1075.369 kb on + strand, within hisD at 1075.369 kb on + strand, within hisD at 1075.370 kb on - strand, within hisD at 1075.370 kb on - strand, within hisD at 1075.370 kb on - strand, within hisD at 1075.370 kb on - strand, within hisD at 1075.370 kb on - strand, within hisD at 1075.387 kb on + strand, within hisD at 1075.387 kb on + strand, within hisD at 1075.387 kb on + strand, within hisD at 1075.387 kb on + strand, within hisD at 1075.388 kb on - strand, within hisD at 1075.388 kb on - strand, within hisD at 1075.388 kb on - strand, within hisD at 1075.411 kb on + strand, within hisD at 1075.412 kb on - strand, within hisD at 1075.412 kb on - strand, within hisD at 1075.596 kb on + strand at 1075.596 kb on + strand at 1075.597 kb on - strand at 1075.597 kb on - strand at 1075.746 kb on + strand, within hisC at 1075.747 kb on - strand, within hisC at 1075.843 kb on + strand, within hisC at 1075.843 kb on + strand, within hisC at 1075.844 kb on - strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.872 kb on + strand, within hisC at 1075.873 kb on - strand, within hisC at 1075.873 kb on - strand, within hisC at 1075.873 kb on - strand, within hisC at 1075.873 kb on - strand, within hisC at 1075.873 kb on - strand, within hisC at 1075.873 kb on - strand, within hisC at 1075.873 kb on - strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.097 kb on + strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.098 kb on - strand, within hisC at 1076.204 kb on + strand, within hisC at 1076.443 kb on + strand, within hisC at 1076.443 kb on + strand, within hisC at 1076.444 kb on - strand, within hisC at 1076.587 kb on + strand at 1076.652 kb on + strand at 1076.652 kb on + strand at 1076.652 kb on + strand at 1076.653 kb on - strand at 1076.653 kb on - strand at 1076.653 kb on - strand at 1076.653 kb on - strand at 1076.714 kb on + strand at 1076.714 kb on + strand at 1076.714 kb on + strand at 1076.715 kb on - strand at 1076.715 kb on - strand at 1076.715 kb on - strand at 1076.975 kb on + strand, within hisB at 1076.976 kb on - strand, within hisB at 1076.976 kb on - strand, within hisB at 1076.976 kb on - strand, within hisB at 1076.976 kb on - strand, within hisB at 1077.467 kb on + strand, within hisB at 1077.467 kb on + strand, within hisB at 1077.467 kb on + strand, within hisB at 1077.467 kb on + strand, within hisB at 1077.468 kb on - strand, within hisB at 1077.468 kb on - strand, within hisB at 1077.468 kb on - strand, within hisB at 1077.504 kb on + strand, within hisB at 1077.505 kb on - strand, within hisB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,074,631 + hisD OKGIIK_04950 0.29 -0.4 1,074,631 + hisD OKGIIK_04950 0.29 -0.3 1,074,631 + hisD OKGIIK_04950 0.29 -0.5 1,074,631 + hisD OKGIIK_04950 0.29 +1.9 1,074,631 + hisD OKGIIK_04950 0.29 -1.9 1,074,631 + hisD OKGIIK_04950 0.29 -1.9 1,074,631 + hisD OKGIIK_04950 0.29 -1.0 1,074,631 + hisD OKGIIK_04950 0.29 -0.4 1,074,632 - hisD OKGIIK_04950 0.29 +0.1 1,074,632 - hisD OKGIIK_04950 0.29 -1.9 1,074,632 - hisD OKGIIK_04950 0.29 -0.1 1,074,632 - hisD OKGIIK_04950 0.29 -0.6 1,074,632 - hisD OKGIIK_04950 0.29 -1.7 1,074,766 + hisD OKGIIK_04950 0.39 -1.7 1,074,767 - hisD OKGIIK_04950 0.40 -1.4 1,074,767 - hisD OKGIIK_04950 0.40 -1.0 1,074,767 - hisD OKGIIK_04950 0.40 -0.4 1,074,767 - hisD OKGIIK_04950 0.40 -1.7 1,074,838 + hisD OKGIIK_04950 0.45 +0.0 1,074,839 - hisD OKGIIK_04950 0.45 +0.8 1,075,336 + hisD OKGIIK_04950 0.83 -1.4 1,075,336 + hisD OKGIIK_04950 0.83 -1.0 1,075,336 + hisD OKGIIK_04950 0.83 -1.2 1,075,336 + hisD OKGIIK_04950 0.83 -0.7 1,075,337 - hisD OKGIIK_04950 0.83 -0.5 1,075,337 - hisD OKGIIK_04950 0.83 -0.7 1,075,369 + hisD OKGIIK_04950 0.86 -0.4 1,075,369 + hisD OKGIIK_04950 0.86 +0.3 1,075,369 + hisD OKGIIK_04950 0.86 -1.7 1,075,369 + hisD OKGIIK_04950 0.86 -1.7 1,075,369 + hisD OKGIIK_04950 0.86 +0.6 1,075,370 - hisD OKGIIK_04950 0.86 +1.6 1,075,370 - hisD OKGIIK_04950 0.86 -0.4 1,075,370 - hisD OKGIIK_04950 0.86 +0.6 1,075,370 - hisD OKGIIK_04950 0.86 +0.1 1,075,370 - hisD OKGIIK_04950 0.86 -0.1 1,075,387 + hisD OKGIIK_04950 0.87 -0.5 1,075,387 + hisD OKGIIK_04950 0.87 -2.1 1,075,387 + hisD OKGIIK_04950 0.87 -1.4 1,075,387 + hisD OKGIIK_04950 0.87 -1.4 1,075,388 - hisD OKGIIK_04950 0.87 +0.3 1,075,388 - hisD OKGIIK_04950 0.87 -1.0 1,075,388 - hisD OKGIIK_04950 0.87 +1.6 1,075,411 + hisD OKGIIK_04950 0.89 +0.4 1,075,412 - hisD OKGIIK_04950 0.89 -0.1 1,075,412 - hisD OKGIIK_04950 0.89 -0.1 1,075,596 + -1.5 1,075,596 + -0.5 1,075,597 - +0.2 1,075,597 - -1.4 1,075,746 + hisC OKGIIK_04955 0.18 +1.3 1,075,747 - hisC OKGIIK_04955 0.18 -1.4 1,075,843 + hisC OKGIIK_04955 0.27 -1.0 1,075,843 + hisC OKGIIK_04955 0.27 -0.4 1,075,844 - hisC OKGIIK_04955 0.27 -0.9 1,075,872 + hisC OKGIIK_04955 0.30 -0.7 1,075,872 + hisC OKGIIK_04955 0.30 +0.6 1,075,872 + hisC OKGIIK_04955 0.30 -0.2 1,075,872 + hisC OKGIIK_04955 0.30 -0.4 1,075,872 + hisC OKGIIK_04955 0.30 -2.1 1,075,872 + hisC OKGIIK_04955 0.30 +0.6 1,075,872 + hisC OKGIIK_04955 0.30 -0.0 1,075,872 + hisC OKGIIK_04955 0.30 -0.1 1,075,872 + hisC OKGIIK_04955 0.30 +0.1 1,075,872 + hisC OKGIIK_04955 0.30 -0.6 1,075,872 + hisC OKGIIK_04955 0.30 -1.5 1,075,872 + hisC OKGIIK_04955 0.30 -1.6 1,075,872 + hisC OKGIIK_04955 0.30 -1.0 1,075,872 + hisC OKGIIK_04955 0.30 -1.7 1,075,872 + hisC OKGIIK_04955 0.30 -0.7 1,075,873 - hisC OKGIIK_04955 0.30 +0.6 1,075,873 - hisC OKGIIK_04955 0.30 -0.9 1,075,873 - hisC OKGIIK_04955 0.30 -1.5 1,075,873 - hisC OKGIIK_04955 0.30 -1.0 1,075,873 - hisC OKGIIK_04955 0.30 -1.2 1,075,873 - hisC OKGIIK_04955 0.30 +0.8 1,075,873 - hisC OKGIIK_04955 0.30 -1.6 1,076,097 + hisC OKGIIK_04955 0.50 -0.3 1,076,097 + hisC OKGIIK_04955 0.50 -0.4 1,076,097 + hisC OKGIIK_04955 0.50 -1.0 1,076,097 + hisC OKGIIK_04955 0.50 -0.0 1,076,097 + hisC OKGIIK_04955 0.50 -0.1 1,076,097 + hisC OKGIIK_04955 0.50 -1.0 1,076,097 + hisC OKGIIK_04955 0.50 -0.7 1,076,097 + hisC OKGIIK_04955 0.50 -1.2 1,076,097 + hisC OKGIIK_04955 0.50 -1.2 1,076,097 + hisC OKGIIK_04955 0.50 -1.6 1,076,097 + hisC OKGIIK_04955 0.50 -0.2 1,076,097 + hisC OKGIIK_04955 0.50 -1.0 1,076,097 + hisC OKGIIK_04955 0.50 +0.4 1,076,097 + hisC OKGIIK_04955 0.50 +0.4 1,076,097 + hisC OKGIIK_04955 0.50 -0.4 1,076,098 - hisC OKGIIK_04955 0.50 -0.4 1,076,098 - hisC OKGIIK_04955 0.50 -0.5 1,076,098 - hisC OKGIIK_04955 0.50 +0.1 1,076,098 - hisC OKGIIK_04955 0.50 -1.7 1,076,098 - hisC OKGIIK_04955 0.50 +0.7 1,076,098 - hisC OKGIIK_04955 0.50 +1.2 1,076,098 - hisC OKGIIK_04955 0.50 -2.0 1,076,098 - hisC OKGIIK_04955 0.50 +0.3 1,076,204 + hisC OKGIIK_04955 0.60 -1.1 1,076,443 + hisC OKGIIK_04955 0.82 -1.2 1,076,443 + hisC OKGIIK_04955 0.82 -2.4 1,076,444 - hisC OKGIIK_04955 0.82 +0.5 1,076,587 + -0.4 1,076,652 + -0.6 1,076,652 + -0.0 1,076,652 + -0.4 1,076,653 - -0.5 1,076,653 - -0.6 1,076,653 - +0.3 1,076,653 - +0.3 1,076,714 + -0.6 1,076,714 + +0.1 1,076,714 + -1.6 1,076,715 - -0.4 1,076,715 - -0.5 1,076,715 - -3.3 1,076,975 + hisB OKGIIK_04960 0.32 -0.7 1,076,976 - hisB OKGIIK_04960 0.32 -0.7 1,076,976 - hisB OKGIIK_04960 0.32 +0.4 1,076,976 - hisB OKGIIK_04960 0.32 -0.0 1,076,976 - hisB OKGIIK_04960 0.32 -1.0 1,077,467 + hisB OKGIIK_04960 0.78 -0.7 1,077,467 + hisB OKGIIK_04960 0.78 +0.3 1,077,467 + hisB OKGIIK_04960 0.78 -0.7 1,077,467 + hisB OKGIIK_04960 0.78 -0.6 1,077,468 - hisB OKGIIK_04960 0.78 -2.4 1,077,468 - hisB OKGIIK_04960 0.78 +0.3 1,077,468 - hisB OKGIIK_04960 0.78 -1.0 1,077,504 + hisB OKGIIK_04960 0.81 -2.9 1,077,505 - hisB OKGIIK_04960 0.81 -1.2
Or see this region's nucleotide sequence