Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt cusA and OKGIIK_04615 are separated by 3 nucleotides OKGIIK_04615 and OKGIIK_04620 are separated by 11 nucleotides OKGIIK_04620 and OKGIIK_04625 overlap by 20 nucleotides OKGIIK_04625 and rhaT are separated by 34 nucleotides
OKGIIK_04610: cusA - CusA/CzcA family heavy metal efflux RND transporter, at 1,006,517 to 1,009,693
cusA
OKGIIK_04615: OKGIIK_04615 - Copper-binding protein, at 1,009,697 to 1,010,053
_04615
OKGIIK_04620: OKGIIK_04620 - hypothetical protein, at 1,010,065 to 1,010,412
_04620
OKGIIK_04625: OKGIIK_04625 - Methyltransferase, at 1,010,393 to 1,011,037
_04625
OKGIIK_04630: rhaT - EamA family transporter, at 1,011,072 to 1,012,001
rhaT
Position (kb)
1010
1011 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 1009.065 kb on + strand, within cusA at 1009.076 kb on - strand, within cusA at 1009.076 kb on - strand, within cusA at 1009.076 kb on - strand, within cusA at 1009.107 kb on - strand, within cusA at 1009.107 kb on - strand, within cusA at 1009.107 kb on - strand, within cusA at 1009.107 kb on - strand, within cusA at 1009.178 kb on + strand, within cusA at 1009.178 kb on + strand, within cusA at 1009.178 kb on + strand, within cusA at 1009.356 kb on - strand, within cusA at 1009.443 kb on + strand at 1009.443 kb on + strand at 1009.444 kb on - strand at 1009.546 kb on - strand at 1009.546 kb on - strand at 1009.601 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.603 kb on - strand at 1009.613 kb on + strand at 1009.642 kb on + strand at 1009.705 kb on - strand at 1009.753 kb on - strand, within OKGIIK_04615 at 1009.753 kb on - strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.842 kb on + strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.843 kb on - strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.876 kb on + strand, within OKGIIK_04615 at 1009.877 kb on - strand, within OKGIIK_04615 at 1009.953 kb on + strand, within OKGIIK_04615 at 1009.953 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1009.983 kb on + strand, within OKGIIK_04615 at 1010.051 kb on + strand at 1010.052 kb on - strand at 1010.052 kb on - strand at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.161 kb on + strand, within OKGIIK_04620 at 1010.324 kb on + strand, within OKGIIK_04620 at 1010.324 kb on + strand, within OKGIIK_04620 at 1010.325 kb on - strand, within OKGIIK_04620 at 1010.325 kb on - strand, within OKGIIK_04620 at 1010.325 kb on - strand, within OKGIIK_04620 at 1010.443 kb on - strand at 1010.443 kb on - strand at 1010.443 kb on - strand at 1010.443 kb on - strand at 1010.522 kb on + strand, within OKGIIK_04625 at 1010.547 kb on + strand, within OKGIIK_04625 at 1010.558 kb on - strand, within OKGIIK_04625 at 1010.652 kb on + strand, within OKGIIK_04625 at 1010.653 kb on - strand, within OKGIIK_04625 at 1010.653 kb on - strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.672 kb on + strand, within OKGIIK_04625 at 1010.673 kb on - strand, within OKGIIK_04625 at 1010.715 kb on + strand, within OKGIIK_04625 at 1010.953 kb on + strand, within OKGIIK_04625 at 1011.193 kb on + strand, within rhaT at 1011.193 kb on + strand, within rhaT at 1011.194 kb on - strand, within rhaT at 1011.194 kb on - strand, within rhaT at 1011.253 kb on + strand, within rhaT at 1011.253 kb on + strand, within rhaT at 1011.254 kb on - strand, within rhaT
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 1,009,065 + cusA OKGIIK_04610 0.80 +0.2 1,009,076 - cusA OKGIIK_04610 0.81 -2.6 1,009,076 - cusA OKGIIK_04610 0.81 -2.0 1,009,076 - cusA OKGIIK_04610 0.81 -0.4 1,009,107 - cusA OKGIIK_04610 0.82 +0.4 1,009,107 - cusA OKGIIK_04610 0.82 -1.1 1,009,107 - cusA OKGIIK_04610 0.82 +0.2 1,009,107 - cusA OKGIIK_04610 0.82 +2.2 1,009,178 + cusA OKGIIK_04610 0.84 +0.8 1,009,178 + cusA OKGIIK_04610 0.84 +0.2 1,009,178 + cusA OKGIIK_04610 0.84 -1.8 1,009,356 - cusA OKGIIK_04610 0.89 +0.2 1,009,443 + -0.4 1,009,443 + -0.8 1,009,444 - -1.8 1,009,546 - +0.7 1,009,546 - -0.4 1,009,601 - +0.2 1,009,603 - -0.9 1,009,603 - +0.4 1,009,603 - +0.2 1,009,603 - -0.4 1,009,603 - -1.4 1,009,603 - -0.5 1,009,613 + +0.4 1,009,642 + -0.4 1,009,705 - -0.3 1,009,753 - OKGIIK_04615 0.16 +0.3 1,009,753 - OKGIIK_04615 0.16 -0.8 1,009,842 + OKGIIK_04615 0.41 -0.2 1,009,842 + OKGIIK_04615 0.41 +0.9 1,009,842 + OKGIIK_04615 0.41 -1.7 1,009,842 + OKGIIK_04615 0.41 +2.0 1,009,842 + OKGIIK_04615 0.41 -0.5 1,009,843 - OKGIIK_04615 0.41 -1.4 1,009,843 - OKGIIK_04615 0.41 +0.4 1,009,843 - OKGIIK_04615 0.41 +0.8 1,009,876 + OKGIIK_04615 0.50 -1.2 1,009,876 + OKGIIK_04615 0.50 -0.1 1,009,876 + OKGIIK_04615 0.50 +0.8 1,009,876 + OKGIIK_04615 0.50 +0.0 1,009,877 - OKGIIK_04615 0.50 -1.0 1,009,953 + OKGIIK_04615 0.72 -2.1 1,009,953 + OKGIIK_04615 0.72 -1.0 1,009,983 + OKGIIK_04615 0.80 -1.1 1,009,983 + OKGIIK_04615 0.80 +0.1 1,009,983 + OKGIIK_04615 0.80 -0.8 1,009,983 + OKGIIK_04615 0.80 +0.2 1,010,051 + -1.4 1,010,052 - -0.4 1,010,052 - +0.6 1,010,161 + OKGIIK_04620 0.28 -0.3 1,010,161 + OKGIIK_04620 0.28 -0.6 1,010,161 + OKGIIK_04620 0.28 -1.6 1,010,161 + OKGIIK_04620 0.28 -0.2 1,010,161 + OKGIIK_04620 0.28 +0.1 1,010,161 + OKGIIK_04620 0.28 -1.0 1,010,161 + OKGIIK_04620 0.28 -0.6 1,010,161 + OKGIIK_04620 0.28 -0.2 1,010,324 + OKGIIK_04620 0.74 -1.8 1,010,324 + OKGIIK_04620 0.74 -0.8 1,010,325 - OKGIIK_04620 0.75 -0.7 1,010,325 - OKGIIK_04620 0.75 +0.2 1,010,325 - OKGIIK_04620 0.75 -1.2 1,010,443 - +0.6 1,010,443 - +0.3 1,010,443 - -1.9 1,010,443 - -0.0 1,010,522 + OKGIIK_04625 0.20 -0.6 1,010,547 + OKGIIK_04625 0.24 -0.9 1,010,558 - OKGIIK_04625 0.26 -0.5 1,010,652 + OKGIIK_04625 0.40 -0.4 1,010,653 - OKGIIK_04625 0.40 -0.8 1,010,653 - OKGIIK_04625 0.40 -0.0 1,010,672 + OKGIIK_04625 0.43 -0.8 1,010,672 + OKGIIK_04625 0.43 +1.0 1,010,672 + OKGIIK_04625 0.43 +0.0 1,010,672 + OKGIIK_04625 0.43 -1.0 1,010,673 - OKGIIK_04625 0.43 +0.8 1,010,715 + OKGIIK_04625 0.50 +1.2 1,010,953 + OKGIIK_04625 0.87 +0.6 1,011,193 + rhaT OKGIIK_04630 0.13 +0.6 1,011,193 + rhaT OKGIIK_04630 0.13 +1.0 1,011,194 - rhaT OKGIIK_04630 0.13 -0.2 1,011,194 - rhaT OKGIIK_04630 0.13 -1.3 1,011,253 + rhaT OKGIIK_04630 0.19 +1.3 1,011,253 + rhaT OKGIIK_04630 0.19 +1.1 1,011,254 - rhaT OKGIIK_04630 0.20 -2.0
Or see this region's nucleotide sequence