Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ubiM and azlC are separated by 74 nucleotides azlC and OKGIIK_04335 overlap by 1 nucleotides OKGIIK_04335 and yhfA are separated by 9 nucleotides yhfA and caiA are separated by 23 nucleotides
OKGIIK_04325: ubiM - 5-demethoxyubiquinol-8 5-hydroxylase UbiM, at 943,089 to 944,315
ubiM
OKGIIK_04330: azlC - putative branched-chain amino acid permease (azaleucine resistance), at 944,390 to 945,130
azlC
OKGIIK_04335: OKGIIK_04335 - branched-chain amino acid transporter AzlD, at 945,130 to 945,441
_04335
OKGIIK_04340: yhfA - peroxiredoxin, at 945,451 to 945,861
yhfA
OKGIIK_04345: caiA - acyl-CoA dehydrogenase, at 945,885 to 947,654
caiA
Position (kb) 944
945
946 Strain fitness (log2 ratio) -2
-1
0
1
2 at 943.434 kb on + strand, within ubiM at 943.434 kb on + strand, within ubiM at 943.435 kb on - strand, within ubiM at 943.435 kb on - strand, within ubiM at 943.435 kb on - strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.743 kb on + strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.744 kb on - strand, within ubiM at 943.905 kb on + strand, within ubiM at 943.906 kb on - strand, within ubiM at 943.906 kb on - strand, within ubiM at 943.906 kb on - strand, within ubiM at 943.906 kb on - strand, within ubiM at 944.121 kb on + strand, within ubiM at 944.121 kb on + strand, within ubiM at 944.121 kb on + strand, within ubiM at 944.122 kb on - strand, within ubiM at 944.122 kb on - strand, within ubiM at 944.122 kb on - strand, within ubiM at 944.122 kb on - strand, within ubiM at 944.122 kb on - strand, within ubiM at 944.151 kb on + strand, within ubiM at 944.151 kb on + strand, within ubiM at 944.151 kb on + strand, within ubiM at 944.151 kb on + strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.152 kb on - strand, within ubiM at 944.153 kb on + strand, within ubiM at 944.154 kb on - strand, within ubiM at 944.154 kb on - strand, within ubiM at 944.154 kb on - strand, within ubiM at 944.154 kb on - strand, within ubiM at 944.378 kb on + strand at 944.454 kb on - strand at 944.636 kb on + strand, within azlC at 944.636 kb on + strand, within azlC at 944.636 kb on + strand, within azlC at 944.636 kb on + strand, within azlC at 945.092 kb on + strand at 945.172 kb on + strand, within OKGIIK_04335 at 945.172 kb on + strand, within OKGIIK_04335 at 945.172 kb on + strand, within OKGIIK_04335 at 945.172 kb on + strand, within OKGIIK_04335 at 945.173 kb on - strand, within OKGIIK_04335 at 945.266 kb on + strand, within OKGIIK_04335 at 945.266 kb on + strand, within OKGIIK_04335 at 945.267 kb on - strand, within OKGIIK_04335 at 945.267 kb on - strand, within OKGIIK_04335 at 945.267 kb on - strand, within OKGIIK_04335 at 945.267 kb on - strand, within OKGIIK_04335 at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.674 kb on + strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.675 kb on - strand, within yhfA at 945.818 kb on + strand, within yhfA at 945.853 kb on + strand at 945.853 kb on + strand at 945.853 kb on + strand at 945.853 kb on + strand at 945.853 kb on + strand at 945.853 kb on + strand at 945.853 kb on + strand at 945.854 kb on - strand at 945.854 kb on - strand at 945.854 kb on - strand at 945.854 kb on - strand at 945.854 kb on - strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.904 kb on + strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand at 945.905 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 943,434 + ubiM OKGIIK_04325 0.28 -2.3 943,434 + ubiM OKGIIK_04325 0.28 +0.2 943,435 - ubiM OKGIIK_04325 0.28 +1.9 943,435 - ubiM OKGIIK_04325 0.28 -0.7 943,435 - ubiM OKGIIK_04325 0.28 -1.8 943,743 + ubiM OKGIIK_04325 0.53 -0.4 943,743 + ubiM OKGIIK_04325 0.53 -0.9 943,743 + ubiM OKGIIK_04325 0.53 +0.4 943,743 + ubiM OKGIIK_04325 0.53 +0.4 943,743 + ubiM OKGIIK_04325 0.53 +0.3 943,743 + ubiM OKGIIK_04325 0.53 +0.6 943,743 + ubiM OKGIIK_04325 0.53 +0.2 943,743 + ubiM OKGIIK_04325 0.53 +0.2 943,743 + ubiM OKGIIK_04325 0.53 -0.2 943,743 + ubiM OKGIIK_04325 0.53 -0.8 943,743 + ubiM OKGIIK_04325 0.53 -0.4 943,743 + ubiM OKGIIK_04325 0.53 -0.2 943,743 + ubiM OKGIIK_04325 0.53 -1.2 943,743 + ubiM OKGIIK_04325 0.53 +0.6 943,743 + ubiM OKGIIK_04325 0.53 -0.0 943,743 + ubiM OKGIIK_04325 0.53 +1.2 943,743 + ubiM OKGIIK_04325 0.53 +0.3 943,743 + ubiM OKGIIK_04325 0.53 -0.3 943,743 + ubiM OKGIIK_04325 0.53 -1.2 943,743 + ubiM OKGIIK_04325 0.53 -0.1 943,743 + ubiM OKGIIK_04325 0.53 +0.2 943,743 + ubiM OKGIIK_04325 0.53 -2.0 943,743 + ubiM OKGIIK_04325 0.53 -1.4 943,743 + ubiM OKGIIK_04325 0.53 -0.9 943,743 + ubiM OKGIIK_04325 0.53 -1.0 943,744 - ubiM OKGIIK_04325 0.53 -0.1 943,744 - ubiM OKGIIK_04325 0.53 +0.8 943,744 - ubiM OKGIIK_04325 0.53 -0.6 943,744 - ubiM OKGIIK_04325 0.53 -0.3 943,744 - ubiM OKGIIK_04325 0.53 -0.6 943,744 - ubiM OKGIIK_04325 0.53 -1.9 943,744 - ubiM OKGIIK_04325 0.53 -1.0 943,744 - ubiM OKGIIK_04325 0.53 +1.0 943,744 - ubiM OKGIIK_04325 0.53 +1.4 943,744 - ubiM OKGIIK_04325 0.53 -1.9 943,744 - ubiM OKGIIK_04325 0.53 -1.0 943,744 - ubiM OKGIIK_04325 0.53 -2.3 943,744 - ubiM OKGIIK_04325 0.53 -0.4 943,744 - ubiM OKGIIK_04325 0.53 -1.9 943,744 - ubiM OKGIIK_04325 0.53 +0.0 943,744 - ubiM OKGIIK_04325 0.53 -0.7 943,744 - ubiM OKGIIK_04325 0.53 +0.9 943,744 - ubiM OKGIIK_04325 0.53 -1.7 943,744 - ubiM OKGIIK_04325 0.53 -0.6 943,744 - ubiM OKGIIK_04325 0.53 -0.2 943,744 - ubiM OKGIIK_04325 0.53 -2.0 943,905 + ubiM OKGIIK_04325 0.67 -0.0 943,906 - ubiM OKGIIK_04325 0.67 -2.5 943,906 - ubiM OKGIIK_04325 0.67 -0.4 943,906 - ubiM OKGIIK_04325 0.67 -1.0 943,906 - ubiM OKGIIK_04325 0.67 +1.0 944,121 + ubiM OKGIIK_04325 0.84 +1.1 944,121 + ubiM OKGIIK_04325 0.84 -0.8 944,121 + ubiM OKGIIK_04325 0.84 +0.8 944,122 - ubiM OKGIIK_04325 0.84 -1.0 944,122 - ubiM OKGIIK_04325 0.84 +0.8 944,122 - ubiM OKGIIK_04325 0.84 -0.1 944,122 - ubiM OKGIIK_04325 0.84 +0.8 944,122 - ubiM OKGIIK_04325 0.84 -0.2 944,151 + ubiM OKGIIK_04325 0.87 +0.6 944,151 + ubiM OKGIIK_04325 0.87 -0.3 944,151 + ubiM OKGIIK_04325 0.87 +0.2 944,151 + ubiM OKGIIK_04325 0.87 +0.4 944,152 - ubiM OKGIIK_04325 0.87 -0.8 944,152 - ubiM OKGIIK_04325 0.87 -0.6 944,152 - ubiM OKGIIK_04325 0.87 -0.5 944,152 - ubiM OKGIIK_04325 0.87 -1.8 944,152 - ubiM OKGIIK_04325 0.87 +0.8 944,152 - ubiM OKGIIK_04325 0.87 -0.4 944,152 - ubiM OKGIIK_04325 0.87 +0.3 944,152 - ubiM OKGIIK_04325 0.87 -0.3 944,152 - ubiM OKGIIK_04325 0.87 +0.8 944,153 + ubiM OKGIIK_04325 0.87 +0.7 944,154 - ubiM OKGIIK_04325 0.87 -0.4 944,154 - ubiM OKGIIK_04325 0.87 -2.0 944,154 - ubiM OKGIIK_04325 0.87 -0.6 944,154 - ubiM OKGIIK_04325 0.87 +1.4 944,378 + -0.9 944,454 - +0.2 944,636 + azlC OKGIIK_04330 0.33 -1.0 944,636 + azlC OKGIIK_04330 0.33 +0.6 944,636 + azlC OKGIIK_04330 0.33 -1.0 944,636 + azlC OKGIIK_04330 0.33 -1.6 945,092 + +0.2 945,172 + OKGIIK_04335 0.13 -0.5 945,172 + OKGIIK_04335 0.13 -1.2 945,172 + OKGIIK_04335 0.13 -0.4 945,172 + OKGIIK_04335 0.13 -0.9 945,173 - OKGIIK_04335 0.14 -1.4 945,266 + OKGIIK_04335 0.44 -1.0 945,266 + OKGIIK_04335 0.44 +0.2 945,267 - OKGIIK_04335 0.44 +0.4 945,267 - OKGIIK_04335 0.44 -1.8 945,267 - OKGIIK_04335 0.44 +1.0 945,267 - OKGIIK_04335 0.44 -2.0 945,674 + yhfA OKGIIK_04340 0.54 -0.8 945,674 + yhfA OKGIIK_04340 0.54 -1.0 945,674 + yhfA OKGIIK_04340 0.54 -0.4 945,674 + yhfA OKGIIK_04340 0.54 +1.1 945,674 + yhfA OKGIIK_04340 0.54 +0.3 945,674 + yhfA OKGIIK_04340 0.54 -1.6 945,674 + yhfA OKGIIK_04340 0.54 -0.3 945,674 + yhfA OKGIIK_04340 0.54 +2.1 945,675 - yhfA OKGIIK_04340 0.55 -0.0 945,675 - yhfA OKGIIK_04340 0.55 -0.4 945,675 - yhfA OKGIIK_04340 0.55 -1.0 945,675 - yhfA OKGIIK_04340 0.55 +1.3 945,675 - yhfA OKGIIK_04340 0.55 -1.1 945,675 - yhfA OKGIIK_04340 0.55 -0.3 945,675 - yhfA OKGIIK_04340 0.55 -0.4 945,675 - yhfA OKGIIK_04340 0.55 -0.2 945,675 - yhfA OKGIIK_04340 0.55 +1.9 945,675 - yhfA OKGIIK_04340 0.55 +1.2 945,675 - yhfA OKGIIK_04340 0.55 +0.9 945,675 - yhfA OKGIIK_04340 0.55 +0.8 945,818 + yhfA OKGIIK_04340 0.89 +0.1 945,853 + +0.8 945,853 + -1.2 945,853 + +1.3 945,853 + +0.8 945,853 + +1.6 945,853 + -0.1 945,853 + +0.6 945,854 - -1.0 945,854 - -0.7 945,854 - +1.3 945,854 - +0.4 945,854 - -2.0 945,904 + -0.9 945,904 + +0.6 945,904 + +0.8 945,904 + +0.4 945,904 + +0.6 945,904 + -0.0 945,904 + +0.8 945,904 + -0.0 945,904 + +0.9 945,905 - -0.9 945,905 - +0.2 945,905 - +0.4 945,905 - -0.2 945,905 - -0.0 945,905 - -0.1 945,905 - +0.4 945,905 - +0.9 945,905 - +0.6 945,905 - +0.5
Or see this region's nucleotide sequence