Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt dapA and OKGIIK_03860 are separated by 51 nucleotides OKGIIK_03860 and OKGIIK_03865 are separated by 65 nucleotides OKGIIK_03865 and Glu_trna are separated by 150 nucleotides Glu_trna and pcnB are separated by 189 nucleotides
OKGIIK_03855: dapA - 4-hydroxy-tetrahydrodipicolinate synthase, at 849,071 to 849,949
dapA
OKGIIK_03860: OKGIIK_03860 - hypothetical protein, at 850,001 to 850,591
_03860
OKGIIK_03865: OKGIIK_03865 - ferredoxin, at 850,657 to 850,980
_03865
OKGIIK_03870: Glu_trna - tRNA-Glu, at 851,131 to 851,206
_trna
OKGIIK_03875: pcnB - tRNA nucleotidyltransferase/poly(A) polymerase, at 851,396 to 852,745
pcnB
Position (kb) 850
851 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 849.947 kb on + strand at 849.947 kb on + strand at 849.947 kb on + strand at 849.947 kb on + strand at 849.981 kb on + strand at 849.981 kb on + strand at 849.981 kb on + strand at 849.982 kb on - strand at 849.998 kb on + strand at 849.998 kb on + strand at 849.998 kb on + strand at 849.999 kb on - strand at 849.999 kb on - strand at 849.999 kb on - strand at 850.286 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.361 kb on + strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.362 kb on - strand, within OKGIIK_03860 at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.565 kb on - strand at 850.814 kb on + strand, within OKGIIK_03865 at 850.940 kb on + strand, within OKGIIK_03865 at 850.941 kb on - strand, within OKGIIK_03865 at 850.941 kb on - strand, within OKGIIK_03865 at 850.941 kb on - strand, within OKGIIK_03865 at 850.941 kb on - strand, within OKGIIK_03865 at 850.997 kb on + strand at 850.997 kb on + strand at 850.997 kb on + strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 850.998 kb on - strand at 851.052 kb on + strand at 851.052 kb on + strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.053 kb on - strand at 851.057 kb on + strand at 851.057 kb on + strand at 851.057 kb on + strand at 851.058 kb on - strand at 851.058 kb on - strand at 851.058 kb on - strand at 851.083 kb on + strand at 851.083 kb on + strand at 851.083 kb on + strand at 851.083 kb on + strand at 851.083 kb on + strand at 851.083 kb on + strand at 851.083 kb on + strand at 851.084 kb on + strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.084 kb on - strand at 851.141 kb on + strand, within Glu_trna at 851.186 kb on - strand, within Glu_trna at 851.193 kb on + strand, within Glu_trna at 851.209 kb on + strand at 851.209 kb on + strand at 851.209 kb on + strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.210 kb on - strand at 851.226 kb on + strand at 851.226 kb on + strand at 851.226 kb on + strand at 851.227 kb on - strand at 851.227 kb on - strand at 851.227 kb on - strand at 851.227 kb on - strand at 851.229 kb on + strand at 851.229 kb on + strand at 851.229 kb on + strand at 851.230 kb on - strand at 851.230 kb on - strand at 851.230 kb on - strand at 851.230 kb on - strand at 851.230 kb on - strand at 851.231 kb on + strand at 851.232 kb on - strand at 851.261 kb on - strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.350 kb on + strand at 851.351 kb on - strand at 851.351 kb on - strand at 851.351 kb on - strand at 851.351 kb on - strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.352 kb on + strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.353 kb on - strand at 851.504 kb on + strand at 851.504 kb on + strand at 851.504 kb on + strand at 851.534 kb on + strand, within pcnB at 851.534 kb on + strand, within pcnB at 851.534 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.843 kb on + strand, within pcnB at 851.846 kb on + strand, within pcnB at 851.846 kb on + strand, within pcnB at 851.876 kb on + strand, within pcnB at 851.876 kb on + strand, within pcnB at 851.876 kb on + strand, within pcnB at 851.910 kb on + strand, within pcnB at 851.910 kb on + strand, within pcnB at 851.910 kb on + strand, within pcnB at 851.911 kb on - strand, within pcnB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 849,947 + -1.6 849,947 + -0.2 849,947 + +0.4 849,947 + +0.4 849,981 + +1.3 849,981 + -1.3 849,981 + +0.3 849,982 - +0.6 849,998 + -1.4 849,998 + +0.8 849,998 + +0.2 849,999 - -0.4 849,999 - -0.0 849,999 - +0.6 850,286 + OKGIIK_03860 0.48 -1.2 850,361 + OKGIIK_03860 0.61 -0.4 850,361 + OKGIIK_03860 0.61 -1.0 850,361 + OKGIIK_03860 0.61 -0.6 850,361 + OKGIIK_03860 0.61 -1.1 850,361 + OKGIIK_03860 0.61 -0.2 850,361 + OKGIIK_03860 0.61 -1.7 850,361 + OKGIIK_03860 0.61 -0.8 850,361 + OKGIIK_03860 0.61 -0.6 850,361 + OKGIIK_03860 0.61 +0.2 850,361 + OKGIIK_03860 0.61 +0.1 850,361 + OKGIIK_03860 0.61 +0.2 850,361 + OKGIIK_03860 0.61 -0.9 850,361 + OKGIIK_03860 0.61 -0.4 850,361 + OKGIIK_03860 0.61 +0.2 850,361 + OKGIIK_03860 0.61 -0.4 850,361 + OKGIIK_03860 0.61 -0.4 850,361 + OKGIIK_03860 0.61 +0.6 850,361 + OKGIIK_03860 0.61 +0.6 850,361 + OKGIIK_03860 0.61 +0.8 850,361 + OKGIIK_03860 0.61 -0.1 850,361 + OKGIIK_03860 0.61 -0.4 850,361 + OKGIIK_03860 0.61 -1.0 850,361 + OKGIIK_03860 0.61 +0.1 850,362 - OKGIIK_03860 0.61 -1.2 850,362 - OKGIIK_03860 0.61 -0.8 850,362 - OKGIIK_03860 0.61 +1.0 850,362 - OKGIIK_03860 0.61 +0.1 850,362 - OKGIIK_03860 0.61 -3.0 850,362 - OKGIIK_03860 0.61 -1.4 850,362 - OKGIIK_03860 0.61 -1.9 850,565 - -1.0 850,565 - +1.1 850,565 - -1.4 850,565 - -0.8 850,565 - +0.6 850,565 - -0.0 850,565 - -0.8 850,565 - +0.2 850,565 - -0.8 850,814 + OKGIIK_03865 0.48 -1.8 850,940 + OKGIIK_03865 0.87 -2.1 850,941 - OKGIIK_03865 0.88 -1.9 850,941 - OKGIIK_03865 0.88 -0.3 850,941 - OKGIIK_03865 0.88 -0.4 850,941 - OKGIIK_03865 0.88 -0.4 850,997 + -0.4 850,997 + +0.4 850,997 + -1.2 850,998 - -0.3 850,998 - -0.9 850,998 - -1.2 850,998 - +0.3 850,998 - -0.7 850,998 - -0.6 850,998 - -0.3 850,998 - -3.0 850,998 - -1.4 851,052 + -1.8 851,052 + +2.1 851,053 - +0.8 851,053 - -0.8 851,053 - -1.2 851,053 - -1.4 851,053 - +0.8 851,053 - -1.6 851,053 - +0.2 851,053 - +2.4 851,053 - -1.8 851,053 - +0.6 851,053 - +1.0 851,057 + +0.6 851,057 + +1.8 851,057 + -0.4 851,058 - +1.6 851,058 - +0.2 851,058 - -0.4 851,083 + -1.8 851,083 + -0.4 851,083 + -1.3 851,083 + +1.6 851,083 + -1.5 851,083 + +0.2 851,083 + -0.4 851,084 + -1.3 851,084 - -0.0 851,084 - +1.9 851,084 - -0.4 851,084 - +0.2 851,084 - -1.9 851,084 - -0.0 851,084 - +0.6 851,084 - -1.1 851,084 - -2.0 851,084 - -1.2 851,084 - +0.3 851,084 - -1.7 851,084 - +0.5 851,084 - -0.1 851,084 - +1.2 851,084 - -0.2 851,084 - +0.3 851,141 + Glu_trna OKGIIK_03870 0.13 +0.6 851,186 - Glu_trna OKGIIK_03870 0.72 -0.6 851,193 + Glu_trna OKGIIK_03870 0.82 +0.4 851,209 + +0.6 851,209 + +1.1 851,209 + -2.1 851,210 - -0.3 851,210 - -0.1 851,210 - -0.4 851,210 - -0.5 851,210 - -0.1 851,210 - -0.1 851,210 - -0.5 851,210 - -1.8 851,226 + +0.8 851,226 + -0.2 851,226 + -0.8 851,227 - -0.8 851,227 - +0.9 851,227 - -1.5 851,227 - -0.2 851,229 + -0.9 851,229 + +0.1 851,229 + -0.4 851,230 - -0.6 851,230 - -2.2 851,230 - -0.9 851,230 - +1.3 851,230 - -0.6 851,231 + -1.8 851,232 - +0.2 851,261 - -1.2 851,350 + -1.0 851,350 + -2.1 851,350 + -0.1 851,350 + -0.4 851,350 + -2.4 851,350 + -0.5 851,350 + -3.0 851,350 + +0.5 851,351 - +0.3 851,351 - -0.2 851,351 - -0.7 851,351 - +1.4 851,352 + -0.4 851,352 + -0.8 851,352 + -0.4 851,352 + -0.0 851,352 + -0.2 851,352 + -0.7 851,352 + +0.2 851,352 + -0.0 851,352 + -0.0 851,352 + +1.1 851,352 + -0.6 851,352 + -1.0 851,352 + -2.2 851,352 + -0.2 851,352 + -0.5 851,352 + -1.8 851,352 + -0.1 851,352 + -2.2 851,353 - +0.7 851,353 - +1.7 851,353 - -2.1 851,353 - -1.0 851,353 - -1.8 851,353 - +0.1 851,353 - -1.9 851,353 - +0.1 851,353 - -0.8 851,504 + +0.2 851,504 + -0.2 851,504 + -0.2 851,534 + pcnB OKGIIK_03875 0.10 -0.4 851,534 + pcnB OKGIIK_03875 0.10 -1.2 851,534 + pcnB OKGIIK_03875 0.10 +0.1 851,843 + pcnB OKGIIK_03875 0.33 -1.4 851,843 + pcnB OKGIIK_03875 0.33 -1.1 851,843 + pcnB OKGIIK_03875 0.33 -2.1 851,843 + pcnB OKGIIK_03875 0.33 +0.2 851,843 + pcnB OKGIIK_03875 0.33 -1.1 851,843 + pcnB OKGIIK_03875 0.33 -1.2 851,843 + pcnB OKGIIK_03875 0.33 -0.0 851,846 + pcnB OKGIIK_03875 0.33 +1.1 851,846 + pcnB OKGIIK_03875 0.33 -0.8 851,876 + pcnB OKGIIK_03875 0.36 +0.9 851,876 + pcnB OKGIIK_03875 0.36 -0.5 851,876 + pcnB OKGIIK_03875 0.36 -0.0 851,910 + pcnB OKGIIK_03875 0.38 -0.4 851,910 + pcnB OKGIIK_03875 0.38 -1.4 851,910 + pcnB OKGIIK_03875 0.38 +1.6 851,911 - pcnB OKGIIK_03875 0.38 -1.7
Or see this region's nucleotide sequence