Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt gluQRS and htpX are separated by 126 nucleotides htpX and htpG are separated by 188 nucleotides
OKGIIK_03640: gluQRS - tRNA glutamyl-Q(34) synthetase GluQRS, at 801,488 to 802,354
gluQRS
OKGIIK_03645: htpX - protease HtpX, at 802,481 to 803,374
htpX
OKGIIK_03650: htpG - molecular chaperone HtpG, at 803,563 to 805,431
htpG
Position (kb) 802
803
804 Strain fitness (log2 ratio) -2
-1
0
1
2 at 801.481 kb on + strand at 801.482 kb on - strand at 801.482 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.523 kb on - strand at 801.717 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.846 kb on + strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.847 kb on - strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on + strand, within gluQRS at 801.852 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 801.853 kb on - strand, within gluQRS at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.365 kb on + strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.366 kb on - strand at 802.613 kb on + strand, within htpX at 802.614 kb on - strand, within htpX at 802.614 kb on - strand, within htpX at 802.796 kb on + strand, within htpX at 802.796 kb on + strand, within htpX at 802.796 kb on + strand, within htpX at 802.797 kb on - strand, within htpX at 802.797 kb on - strand, within htpX at 803.078 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.251 kb on + strand, within htpX at 803.252 kb on - strand, within htpX at 803.252 kb on - strand, within htpX at 803.252 kb on - strand, within htpX at 803.252 kb on - strand, within htpX at 803.399 kb on + strand at 803.399 kb on + strand at 803.399 kb on + strand at 803.400 kb on - strand at 803.509 kb on + strand at 803.509 kb on + strand at 803.509 kb on + strand at 803.509 kb on + strand at 803.509 kb on + strand at 803.509 kb on + strand at 803.510 kb on - strand at 803.510 kb on - strand at 803.510 kb on - strand at 803.535 kb on - strand at 803.535 kb on - strand at 803.638 kb on + strand at 803.639 kb on - strand at 803.639 kb on - strand at 803.639 kb on - strand at 803.639 kb on - strand at 803.722 kb on - strand at 803.947 kb on + strand, within htpG at 803.947 kb on + strand, within htpG at 803.947 kb on + strand, within htpG at 803.948 kb on - strand, within htpG at 803.948 kb on - strand, within htpG at 804.049 kb on + strand, within htpG at 804.049 kb on + strand, within htpG at 804.049 kb on + strand, within htpG at 804.050 kb on - strand, within htpG at 804.164 kb on - strand, within htpG at 804.295 kb on + strand, within htpG at 804.295 kb on + strand, within htpG at 804.295 kb on + strand, within htpG at 804.296 kb on - strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.307 kb on + strand, within htpG at 804.308 kb on - strand, within htpG at 804.308 kb on - strand, within htpG at 804.308 kb on - strand, within htpG at 804.308 kb on - strand, within htpG at 804.308 kb on - strand, within htpG at 804.308 kb on - strand, within htpG
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 801,481 + +0.8 801,482 - +0.8 801,482 - +0.9 801,523 - +0.1 801,523 - +0.8 801,523 - -0.0 801,523 - +0.6 801,523 - +1.3 801,717 + gluQRS OKGIIK_03640 0.26 -0.5 801,846 + gluQRS OKGIIK_03640 0.41 -1.1 801,846 + gluQRS OKGIIK_03640 0.41 +0.6 801,846 + gluQRS OKGIIK_03640 0.41 +0.4 801,846 + gluQRS OKGIIK_03640 0.41 +0.7 801,846 + gluQRS OKGIIK_03640 0.41 -0.6 801,846 + gluQRS OKGIIK_03640 0.41 +0.4 801,846 + gluQRS OKGIIK_03640 0.41 +1.2 801,847 - gluQRS OKGIIK_03640 0.41 +0.6 801,847 - gluQRS OKGIIK_03640 0.41 +0.6 801,847 - gluQRS OKGIIK_03640 0.41 -0.2 801,847 - gluQRS OKGIIK_03640 0.41 +0.3 801,847 - gluQRS OKGIIK_03640 0.41 +0.2 801,847 - gluQRS OKGIIK_03640 0.41 -1.1 801,847 - gluQRS OKGIIK_03640 0.41 -1.6 801,847 - gluQRS OKGIIK_03640 0.41 +0.2 801,847 - gluQRS OKGIIK_03640 0.41 -0.1 801,852 + gluQRS OKGIIK_03640 0.42 +0.1 801,852 + gluQRS OKGIIK_03640 0.42 -0.0 801,852 + gluQRS OKGIIK_03640 0.42 -0.3 801,852 + gluQRS OKGIIK_03640 0.42 -1.6 801,852 + gluQRS OKGIIK_03640 0.42 -1.6 801,852 - gluQRS OKGIIK_03640 0.42 -1.4 801,853 - gluQRS OKGIIK_03640 0.42 -0.4 801,853 - gluQRS OKGIIK_03640 0.42 +0.8 801,853 - gluQRS OKGIIK_03640 0.42 +1.0 801,853 - gluQRS OKGIIK_03640 0.42 +1.0 801,853 - gluQRS OKGIIK_03640 0.42 +2.4 801,853 - gluQRS OKGIIK_03640 0.42 +0.6 801,853 - gluQRS OKGIIK_03640 0.42 +0.6 801,853 - gluQRS OKGIIK_03640 0.42 +0.1 801,853 - gluQRS OKGIIK_03640 0.42 -0.3 801,853 - gluQRS OKGIIK_03640 0.42 +0.7 802,365 + +0.3 802,365 + -1.6 802,365 + -0.2 802,365 + -2.0 802,365 + +0.9 802,365 + -0.1 802,365 + -0.0 802,365 + +1.4 802,365 + -1.1 802,365 + +0.2 802,366 - -0.9 802,366 - +0.9 802,366 - -0.4 802,366 - -0.5 802,366 - -0.3 802,366 - -1.0 802,613 + htpX OKGIIK_03645 0.15 -0.8 802,614 - htpX OKGIIK_03645 0.15 -2.0 802,614 - htpX OKGIIK_03645 0.15 +0.2 802,796 + htpX OKGIIK_03645 0.35 -0.8 802,796 + htpX OKGIIK_03645 0.35 +1.2 802,796 + htpX OKGIIK_03645 0.35 +1.6 802,797 - htpX OKGIIK_03645 0.35 +0.6 802,797 - htpX OKGIIK_03645 0.35 -0.2 803,078 + htpX OKGIIK_03645 0.67 -0.6 803,251 + htpX OKGIIK_03645 0.86 -0.7 803,251 + htpX OKGIIK_03645 0.86 -0.8 803,251 + htpX OKGIIK_03645 0.86 +0.4 803,251 + htpX OKGIIK_03645 0.86 -1.2 803,252 - htpX OKGIIK_03645 0.86 -0.0 803,252 - htpX OKGIIK_03645 0.86 -0.3 803,252 - htpX OKGIIK_03645 0.86 -0.8 803,252 - htpX OKGIIK_03645 0.86 -0.4 803,399 + +0.2 803,399 + -0.4 803,399 + -0.8 803,400 - +0.5 803,509 + +1.7 803,509 + +1.2 803,509 + -1.2 803,509 + -0.9 803,509 + -1.4 803,509 + -1.3 803,510 - -0.2 803,510 - +0.3 803,510 - -0.4 803,535 - +1.3 803,535 - +1.3 803,638 + +1.2 803,639 - -1.8 803,639 - +0.8 803,639 - -0.0 803,639 - +1.3 803,722 - +0.4 803,947 + htpG OKGIIK_03650 0.21 -0.7 803,947 + htpG OKGIIK_03650 0.21 -1.5 803,947 + htpG OKGIIK_03650 0.21 +2.3 803,948 - htpG OKGIIK_03650 0.21 -0.4 803,948 - htpG OKGIIK_03650 0.21 -0.8 804,049 + htpG OKGIIK_03650 0.26 -0.0 804,049 + htpG OKGIIK_03650 0.26 +0.3 804,049 + htpG OKGIIK_03650 0.26 +0.6 804,050 - htpG OKGIIK_03650 0.26 -0.3 804,164 - htpG OKGIIK_03650 0.32 -0.1 804,295 + htpG OKGIIK_03650 0.39 -0.0 804,295 + htpG OKGIIK_03650 0.39 +0.6 804,295 + htpG OKGIIK_03650 0.39 -0.6 804,296 - htpG OKGIIK_03650 0.39 -0.8 804,307 + htpG OKGIIK_03650 0.40 -0.8 804,307 + htpG OKGIIK_03650 0.40 -0.5 804,307 + htpG OKGIIK_03650 0.40 -0.9 804,307 + htpG OKGIIK_03650 0.40 +0.2 804,307 + htpG OKGIIK_03650 0.40 -0.4 804,307 + htpG OKGIIK_03650 0.40 +0.6 804,307 + htpG OKGIIK_03650 0.40 -1.8 804,307 + htpG OKGIIK_03650 0.40 -0.9 804,307 + htpG OKGIIK_03650 0.40 +0.0 804,308 - htpG OKGIIK_03650 0.40 +0.2 804,308 - htpG OKGIIK_03650 0.40 -0.3 804,308 - htpG OKGIIK_03650 0.40 -1.8 804,308 - htpG OKGIIK_03650 0.40 +1.6 804,308 - htpG OKGIIK_03650 0.40 -1.6 804,308 - htpG OKGIIK_03650 0.40 -0.9
Or see this region's nucleotide sequence