Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt nlpA and metP are separated by 227 nucleotides metP and abcC overlap by 4 nucleotides abcC and btuE are separated by 99 nucleotides btuE and OKGIIK_03570 are separated by 121 nucleotides
OKGIIK_03550: nlpA - methionine ABC transporter substrate-binding protein, at 787,191 to 787,985
nlpA
OKGIIK_03555: metP - metal ABC transporter permease, at 788,213 to 788,890
metP
OKGIIK_03560: abcC - methionine ABC transporter ATP-binding protein, at 788,887 to 789,897
abcC
OKGIIK_03565: btuE - glutathione peroxidase, at 789,997 to 790,482
btuE
OKGIIK_03570: OKGIIK_03570 - Peptidyl-prolyl cis-trans isomerase, at 790,604 to 791,086
_03570
Position (kb) 788
789
790 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 787.990 kb on + strand at 787.990 kb on + strand at 787.991 kb on - strand at 787.991 kb on - strand at 788.015 kb on + strand at 788.015 kb on + strand at 788.016 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.025 kb on - strand at 788.027 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.054 kb on - strand at 788.089 kb on - strand at 788.178 kb on + strand at 788.178 kb on + strand at 788.178 kb on + strand at 788.179 kb on - strand at 788.179 kb on - strand at 788.179 kb on - strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.224 kb on + strand at 788.225 kb on - strand at 788.226 kb on + strand at 788.226 kb on + strand at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.304 kb on + strand, within metP at 788.305 kb on - strand, within metP at 788.316 kb on + strand, within metP at 788.316 kb on + strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.317 kb on - strand, within metP at 788.338 kb on - strand, within metP at 788.388 kb on + strand, within metP at 788.628 kb on + strand, within metP at 788.628 kb on + strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.629 kb on - strand, within metP at 788.709 kb on + strand, within metP at 788.751 kb on + strand, within metP at 788.752 kb on - strand, within metP at 788.752 kb on - strand, within metP at 789.274 kb on + strand, within abcC at 789.485 kb on + strand, within abcC at 789.486 kb on - strand, within abcC at 789.486 kb on - strand, within abcC at 789.754 kb on + strand, within abcC at 789.755 kb on - strand, within abcC at 789.755 kb on - strand, within abcC at 789.867 kb on - strand at 789.867 kb on - strand at 789.931 kb on + strand at 789.931 kb on + strand at 789.931 kb on + strand at 789.931 kb on + strand at 789.931 kb on + strand at 789.931 kb on + strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.932 kb on - strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.959 kb on + strand at 789.960 kb on - strand at 789.960 kb on - strand at 789.960 kb on - strand at 789.960 kb on - strand at 789.960 kb on - strand at 789.960 kb on - strand at 789.960 kb on - strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.009 kb on + strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.010 kb on - strand at 790.120 kb on + strand, within btuE at 790.252 kb on + strand, within btuE at 790.252 kb on + strand, within btuE at 790.253 kb on - strand, within btuE at 790.253 kb on - strand, within btuE at 790.253 kb on - strand, within btuE at 790.253 kb on - strand, within btuE at 790.253 kb on - strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.315 kb on + strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.316 kb on - strand, within btuE at 790.420 kb on + strand, within btuE at 790.421 kb on - strand, within btuE at 790.421 kb on - strand, within btuE at 790.421 kb on - strand, within btuE at 790.421 kb on - strand, within btuE at 790.543 kb on + strand at 790.544 kb on - strand at 790.544 kb on - strand at 790.544 kb on - strand at 790.640 kb on + strand at 790.640 kb on + strand at 790.640 kb on + strand at 790.640 kb on + strand at 790.640 kb on + strand at 790.640 kb on + strand at 790.640 kb on + strand at 790.641 kb on - strand at 790.641 kb on - strand at 790.641 kb on - strand at 790.641 kb on - strand at 790.701 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.703 kb on + strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.704 kb on - strand, within OKGIIK_03570 at 790.777 kb on + strand, within OKGIIK_03570 at 790.777 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.805 kb on + strand, within OKGIIK_03570 at 790.806 kb on - strand, within OKGIIK_03570 at 790.806 kb on - strand, within OKGIIK_03570 at 790.806 kb on - strand, within OKGIIK_03570
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 787,990 + +0.4 787,990 + -0.0 787,991 - -0.0 787,991 - -0.9 788,015 + +1.2 788,015 + -1.6 788,016 - -1.8 788,025 - +0.1 788,025 - +0.3 788,025 - -1.0 788,025 - +2.0 788,027 - +2.0 788,054 - -0.4 788,054 - -1.6 788,054 - +1.2 788,054 - -1.4 788,054 - +0.0 788,089 - +0.5 788,178 + -1.8 788,178 + -1.0 788,178 + -0.4 788,179 - -0.6 788,179 - -0.9 788,179 - -0.4 788,224 + -1.0 788,224 + -1.2 788,224 + -0.2 788,224 + -1.8 788,224 + -0.2 788,225 - +0.4 788,226 + -1.1 788,226 + +0.6 788,304 + metP OKGIIK_03555 0.13 -0.4 788,304 + metP OKGIIK_03555 0.13 -0.4 788,304 + metP OKGIIK_03555 0.13 -1.6 788,305 - metP OKGIIK_03555 0.14 -1.2 788,316 + metP OKGIIK_03555 0.15 -0.2 788,316 + metP OKGIIK_03555 0.15 -1.2 788,317 - metP OKGIIK_03555 0.15 -1.2 788,317 - metP OKGIIK_03555 0.15 +0.3 788,317 - metP OKGIIK_03555 0.15 -0.2 788,338 - metP OKGIIK_03555 0.18 -0.6 788,388 + metP OKGIIK_03555 0.26 -0.4 788,628 + metP OKGIIK_03555 0.61 -1.0 788,628 + metP OKGIIK_03555 0.61 -2.2 788,629 - metP OKGIIK_03555 0.61 -0.2 788,629 - metP OKGIIK_03555 0.61 +0.1 788,629 - metP OKGIIK_03555 0.61 -1.9 788,629 - metP OKGIIK_03555 0.61 -2.5 788,629 - metP OKGIIK_03555 0.61 +1.0 788,709 + metP OKGIIK_03555 0.73 -2.0 788,751 + metP OKGIIK_03555 0.79 -2.0 788,752 - metP OKGIIK_03555 0.79 -1.4 788,752 - metP OKGIIK_03555 0.79 -1.8 789,274 + abcC OKGIIK_03560 0.38 -2.0 789,485 + abcC OKGIIK_03560 0.59 -1.3 789,486 - abcC OKGIIK_03560 0.59 -0.2 789,486 - abcC OKGIIK_03560 0.59 -0.0 789,754 + abcC OKGIIK_03560 0.86 -1.3 789,755 - abcC OKGIIK_03560 0.86 -2.0 789,755 - abcC OKGIIK_03560 0.86 -1.6 789,867 - -0.8 789,867 - -0.4 789,931 + -0.4 789,931 + +0.4 789,931 + +0.4 789,931 + -0.0 789,931 + -0.2 789,931 + -2.2 789,932 - +0.3 789,932 - -0.4 789,932 - -2.7 789,932 - -0.3 789,932 - -1.2 789,932 - -1.0 789,932 - +0.6 789,932 - -0.0 789,959 + +2.3 789,959 + -1.2 789,959 + -0.2 789,959 + +0.4 789,959 + -0.6 789,959 + -1.4 789,959 + -0.8 789,959 + -1.2 789,959 + -0.3 789,959 + -1.8 789,960 - -0.8 789,960 - +0.8 789,960 - -0.4 789,960 - +0.8 789,960 - +0.1 789,960 - -0.9 789,960 - +0.9 790,009 + +1.2 790,009 + -0.0 790,009 + -1.6 790,009 + -0.7 790,009 + -0.6 790,009 + -0.0 790,009 + -1.2 790,009 + -2.9 790,009 + +0.2 790,010 - -1.4 790,010 - -0.4 790,010 - -0.3 790,010 - +0.1 790,010 - -1.6 790,010 - -2.1 790,010 - +0.6 790,010 - +0.4 790,010 - -1.2 790,010 - -0.6 790,120 + btuE OKGIIK_03565 0.25 -0.1 790,252 + btuE OKGIIK_03565 0.52 +0.1 790,252 + btuE OKGIIK_03565 0.52 -1.0 790,253 - btuE OKGIIK_03565 0.53 -0.4 790,253 - btuE OKGIIK_03565 0.53 +0.6 790,253 - btuE OKGIIK_03565 0.53 +0.1 790,253 - btuE OKGIIK_03565 0.53 -1.0 790,253 - btuE OKGIIK_03565 0.53 -0.8 790,315 + btuE OKGIIK_03565 0.65 +0.2 790,315 + btuE OKGIIK_03565 0.65 -0.8 790,315 + btuE OKGIIK_03565 0.65 -0.8 790,315 + btuE OKGIIK_03565 0.65 -0.9 790,315 + btuE OKGIIK_03565 0.65 -1.6 790,315 + btuE OKGIIK_03565 0.65 -1.2 790,315 + btuE OKGIIK_03565 0.65 +1.1 790,315 + btuE OKGIIK_03565 0.65 +0.2 790,315 + btuE OKGIIK_03565 0.65 +0.1 790,315 + btuE OKGIIK_03565 0.65 -1.0 790,316 - btuE OKGIIK_03565 0.66 -0.2 790,316 - btuE OKGIIK_03565 0.66 -0.3 790,316 - btuE OKGIIK_03565 0.66 +0.3 790,316 - btuE OKGIIK_03565 0.66 -0.1 790,316 - btuE OKGIIK_03565 0.66 +0.5 790,316 - btuE OKGIIK_03565 0.66 +0.8 790,316 - btuE OKGIIK_03565 0.66 +0.7 790,316 - btuE OKGIIK_03565 0.66 +1.4 790,316 - btuE OKGIIK_03565 0.66 -2.0 790,420 + btuE OKGIIK_03565 0.87 -0.3 790,421 - btuE OKGIIK_03565 0.87 -0.8 790,421 - btuE OKGIIK_03565 0.87 +1.6 790,421 - btuE OKGIIK_03565 0.87 +0.7 790,421 - btuE OKGIIK_03565 0.87 -0.2 790,543 + -1.6 790,544 - -0.1 790,544 - -1.0 790,544 - -1.3 790,640 + +0.7 790,640 + -0.6 790,640 + -0.4 790,640 + +0.2 790,640 + +1.5 790,640 + +0.2 790,640 + +0.1 790,641 - +0.1 790,641 - -0.1 790,641 - +0.6 790,641 - -2.6 790,701 + OKGIIK_03570 0.20 +0.1 790,703 + OKGIIK_03570 0.20 +0.4 790,703 + OKGIIK_03570 0.20 +0.3 790,703 + OKGIIK_03570 0.20 +0.0 790,703 + OKGIIK_03570 0.20 +0.2 790,703 + OKGIIK_03570 0.20 -1.2 790,703 + OKGIIK_03570 0.20 +0.6 790,703 + OKGIIK_03570 0.20 +0.6 790,703 + OKGIIK_03570 0.20 +2.4 790,703 + OKGIIK_03570 0.20 +0.9 790,703 + OKGIIK_03570 0.20 +0.6 790,704 - OKGIIK_03570 0.21 -2.1 790,704 - OKGIIK_03570 0.21 -0.4 790,704 - OKGIIK_03570 0.21 -1.3 790,704 - OKGIIK_03570 0.21 +1.0 790,704 - OKGIIK_03570 0.21 -0.7 790,704 - OKGIIK_03570 0.21 -0.2 790,704 - OKGIIK_03570 0.21 -1.3 790,777 + OKGIIK_03570 0.36 -1.2 790,777 + OKGIIK_03570 0.36 +0.1 790,805 + OKGIIK_03570 0.42 -1.0 790,805 + OKGIIK_03570 0.42 -0.4 790,805 + OKGIIK_03570 0.42 +0.2 790,805 + OKGIIK_03570 0.42 +0.4 790,805 + OKGIIK_03570 0.42 -0.3 790,805 + OKGIIK_03570 0.42 -1.2 790,805 + OKGIIK_03570 0.42 -2.3 790,806 - OKGIIK_03570 0.42 -1.6 790,806 - OKGIIK_03570 0.42 -2.1 790,806 - OKGIIK_03570 0.42 -0.9
Or see this region's nucleotide sequence