Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_03280 and yciW are separated by 34 nucleotides yciW and OKGIIK_03290 are separated by 131 nucleotides OKGIIK_03290 and OKGIIK_03295 overlap by 35 nucleotides OKGIIK_03295 and minE are separated by 91 nucleotides
OKGIIK_03280: OKGIIK_03280 - Carboxyvinyl-carboxyphosphonate phosphorylmutase, at 740,327 to 741,166
_03280
OKGIIK_03285: yciW - carboxymuconolactone decarboxylase, at 741,201 to 741,662
yciW
OKGIIK_03290: OKGIIK_03290 - Cation transport regulator, at 741,794 to 742,072
_03290
OKGIIK_03295: OKGIIK_03295 - hypothetical protein, at 742,038 to 742,541
_03295
OKGIIK_03300: minE - cell division topological specificity factor MinE, at 742,633 to 742,929
minE
Position (kb) 741
742 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 740.283 kb on + strand at 740.283 kb on + strand at 740.284 kb on - strand at 740.284 kb on - strand at 740.498 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.867 kb on + strand, within OKGIIK_03280 at 740.894 kb on + strand, within OKGIIK_03280 at 740.895 kb on - strand, within OKGIIK_03280 at 740.895 kb on - strand, within OKGIIK_03280 at 741.001 kb on + strand, within OKGIIK_03280 at 741.002 kb on - strand, within OKGIIK_03280 at 741.002 kb on - strand, within OKGIIK_03280 at 741.131 kb on + strand at 741.131 kb on + strand at 741.131 kb on + strand at 741.132 kb on - strand at 741.212 kb on + strand at 741.212 kb on + strand at 741.213 kb on - strand at 741.213 kb on - strand at 741.220 kb on - strand at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.270 kb on + strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.271 kb on - strand, within yciW at 741.348 kb on + strand, within yciW at 741.349 kb on - strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.412 kb on + strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.413 kb on - strand, within yciW at 741.441 kb on + strand, within yciW at 741.441 kb on + strand, within yciW at 741.522 kb on + strand, within yciW at 741.522 kb on + strand, within yciW at 741.522 kb on + strand, within yciW at 741.522 kb on + strand, within yciW at 741.522 kb on + strand, within yciW at 741.523 kb on - strand, within yciW at 741.523 kb on - strand, within yciW at 741.639 kb on + strand at 741.639 kb on + strand at 741.639 kb on + strand at 741.639 kb on + strand at 741.640 kb on - strand at 741.800 kb on + strand at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.866 kb on + strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.867 kb on - strand, within OKGIIK_03290 at 741.899 kb on + strand, within OKGIIK_03290 at 741.899 kb on + strand, within OKGIIK_03290 at 741.900 kb on - strand, within OKGIIK_03290 at 741.900 kb on - strand, within OKGIIK_03290 at 741.900 kb on - strand, within OKGIIK_03290 at 741.952 kb on + strand, within OKGIIK_03290 at 741.953 kb on - strand, within OKGIIK_03290 at 741.953 kb on - strand, within OKGIIK_03290 at 741.953 kb on - strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.983 kb on + strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 741.984 kb on - strand, within OKGIIK_03290 at 742.056 kb on + strand at 742.057 kb on - strand at 742.138 kb on + strand, within OKGIIK_03295 at 742.138 kb on + strand, within OKGIIK_03295 at 742.139 kb on - strand, within OKGIIK_03295 at 742.349 kb on - strand, within OKGIIK_03295
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 740,283 + -0.6 740,283 + +1.2 740,284 - -0.9 740,284 - +0.7 740,498 + OKGIIK_03280 0.20 -0.3 740,867 + OKGIIK_03280 0.64 -0.7 740,867 + OKGIIK_03280 0.64 -2.7 740,867 + OKGIIK_03280 0.64 -1.3 740,867 + OKGIIK_03280 0.64 -0.6 740,867 + OKGIIK_03280 0.64 -0.9 740,867 + OKGIIK_03280 0.64 -0.6 740,867 + OKGIIK_03280 0.64 -0.6 740,867 + OKGIIK_03280 0.64 +1.1 740,894 + OKGIIK_03280 0.68 +0.8 740,895 - OKGIIK_03280 0.68 +0.1 740,895 - OKGIIK_03280 0.68 -0.4 741,001 + OKGIIK_03280 0.80 -0.1 741,002 - OKGIIK_03280 0.80 -0.5 741,002 - OKGIIK_03280 0.80 -1.3 741,131 + +0.3 741,131 + -1.5 741,131 + +0.0 741,132 - -1.6 741,212 + -2.4 741,212 + +0.1 741,213 - -0.5 741,213 - -0.8 741,220 - -3.2 741,270 + yciW OKGIIK_03285 0.15 +0.5 741,270 + yciW OKGIIK_03285 0.15 +0.4 741,270 + yciW OKGIIK_03285 0.15 -0.5 741,270 + yciW OKGIIK_03285 0.15 -0.5 741,270 + yciW OKGIIK_03285 0.15 -1.3 741,270 + yciW OKGIIK_03285 0.15 -0.6 741,270 + yciW OKGIIK_03285 0.15 +0.0 741,270 + yciW OKGIIK_03285 0.15 -2.0 741,270 + yciW OKGIIK_03285 0.15 -1.3 741,270 + yciW OKGIIK_03285 0.15 +0.8 741,270 + yciW OKGIIK_03285 0.15 +1.9 741,270 + yciW OKGIIK_03285 0.15 +2.0 741,270 + yciW OKGIIK_03285 0.15 -1.6 741,270 + yciW OKGIIK_03285 0.15 +1.2 741,270 + yciW OKGIIK_03285 0.15 +0.5 741,270 + yciW OKGIIK_03285 0.15 -0.2 741,270 + yciW OKGIIK_03285 0.15 -0.8 741,270 + yciW OKGIIK_03285 0.15 -0.9 741,270 + yciW OKGIIK_03285 0.15 +1.2 741,270 + yciW OKGIIK_03285 0.15 +0.5 741,270 + yciW OKGIIK_03285 0.15 +0.1 741,270 + yciW OKGIIK_03285 0.15 +1.7 741,270 + yciW OKGIIK_03285 0.15 -0.8 741,270 + yciW OKGIIK_03285 0.15 +0.2 741,270 + yciW OKGIIK_03285 0.15 -1.0 741,270 + yciW OKGIIK_03285 0.15 -0.2 741,270 + yciW OKGIIK_03285 0.15 -0.6 741,271 - yciW OKGIIK_03285 0.15 -2.6 741,271 - yciW OKGIIK_03285 0.15 +1.2 741,271 - yciW OKGIIK_03285 0.15 -0.5 741,271 - yciW OKGIIK_03285 0.15 +0.4 741,271 - yciW OKGIIK_03285 0.15 -0.5 741,271 - yciW OKGIIK_03285 0.15 +0.1 741,271 - yciW OKGIIK_03285 0.15 -1.0 741,271 - yciW OKGIIK_03285 0.15 +0.3 741,271 - yciW OKGIIK_03285 0.15 -0.7 741,271 - yciW OKGIIK_03285 0.15 -1.1 741,271 - yciW OKGIIK_03285 0.15 -0.1 741,271 - yciW OKGIIK_03285 0.15 +0.2 741,348 + yciW OKGIIK_03285 0.32 -1.0 741,349 - yciW OKGIIK_03285 0.32 -0.8 741,412 + yciW OKGIIK_03285 0.46 +0.8 741,412 + yciW OKGIIK_03285 0.46 -0.4 741,412 + yciW OKGIIK_03285 0.46 -0.0 741,412 + yciW OKGIIK_03285 0.46 -0.5 741,412 + yciW OKGIIK_03285 0.46 -0.3 741,412 + yciW OKGIIK_03285 0.46 -1.3 741,412 + yciW OKGIIK_03285 0.46 -1.0 741,412 + yciW OKGIIK_03285 0.46 +0.7 741,412 + yciW OKGIIK_03285 0.46 -2.6 741,413 - yciW OKGIIK_03285 0.46 +0.9 741,413 - yciW OKGIIK_03285 0.46 +0.1 741,413 - yciW OKGIIK_03285 0.46 -0.9 741,413 - yciW OKGIIK_03285 0.46 -1.5 741,413 - yciW OKGIIK_03285 0.46 -0.1 741,413 - yciW OKGIIK_03285 0.46 +1.3 741,413 - yciW OKGIIK_03285 0.46 -1.0 741,413 - yciW OKGIIK_03285 0.46 -0.5 741,441 + yciW OKGIIK_03285 0.52 -0.8 741,441 + yciW OKGIIK_03285 0.52 -2.2 741,522 + yciW OKGIIK_03285 0.69 -1.5 741,522 + yciW OKGIIK_03285 0.69 -0.8 741,522 + yciW OKGIIK_03285 0.69 -0.6 741,522 + yciW OKGIIK_03285 0.69 +0.3 741,522 + yciW OKGIIK_03285 0.69 -0.3 741,523 - yciW OKGIIK_03285 0.70 -1.4 741,523 - yciW OKGIIK_03285 0.70 -1.3 741,639 + -0.6 741,639 + -1.2 741,639 + -0.2 741,639 + +0.4 741,640 - -0.7 741,800 + -0.1 741,866 + OKGIIK_03290 0.26 +0.2 741,866 + OKGIIK_03290 0.26 -1.4 741,866 + OKGIIK_03290 0.26 -0.2 741,866 + OKGIIK_03290 0.26 -0.0 741,866 + OKGIIK_03290 0.26 +0.5 741,866 + OKGIIK_03290 0.26 -1.0 741,866 + OKGIIK_03290 0.26 -0.4 741,866 + OKGIIK_03290 0.26 +0.3 741,866 + OKGIIK_03290 0.26 +0.1 741,866 + OKGIIK_03290 0.26 +0.3 741,867 - OKGIIK_03290 0.26 -0.0 741,867 - OKGIIK_03290 0.26 +0.2 741,867 - OKGIIK_03290 0.26 -0.6 741,867 - OKGIIK_03290 0.26 +0.7 741,867 - OKGIIK_03290 0.26 +0.5 741,867 - OKGIIK_03290 0.26 -0.3 741,867 - OKGIIK_03290 0.26 -0.2 741,899 + OKGIIK_03290 0.38 +0.4 741,899 + OKGIIK_03290 0.38 +0.2 741,900 - OKGIIK_03290 0.38 +0.3 741,900 - OKGIIK_03290 0.38 -0.6 741,900 - OKGIIK_03290 0.38 -0.6 741,952 + OKGIIK_03290 0.57 +1.5 741,953 - OKGIIK_03290 0.57 -1.3 741,953 - OKGIIK_03290 0.57 -0.1 741,953 - OKGIIK_03290 0.57 +1.3 741,983 + OKGIIK_03290 0.68 +0.6 741,983 + OKGIIK_03290 0.68 +0.4 741,983 + OKGIIK_03290 0.68 +0.7 741,983 + OKGIIK_03290 0.68 -1.3 741,983 + OKGIIK_03290 0.68 -0.6 741,983 + OKGIIK_03290 0.68 -0.2 741,983 + OKGIIK_03290 0.68 -1.0 741,983 + OKGIIK_03290 0.68 -0.0 741,984 - OKGIIK_03290 0.68 -1.5 741,984 - OKGIIK_03290 0.68 +0.3 741,984 - OKGIIK_03290 0.68 +0.0 741,984 - OKGIIK_03290 0.68 +0.5 741,984 - OKGIIK_03290 0.68 -0.5 741,984 - OKGIIK_03290 0.68 -0.5 741,984 - OKGIIK_03290 0.68 +1.3 741,984 - OKGIIK_03290 0.68 +0.4 741,984 - OKGIIK_03290 0.68 -0.0 741,984 - OKGIIK_03290 0.68 +0.2 742,056 + -0.4 742,057 - +0.5 742,138 + OKGIIK_03295 0.20 -0.2 742,138 + OKGIIK_03295 0.20 +2.7 742,139 - OKGIIK_03295 0.20 -0.5 742,349 - OKGIIK_03295 0.62 +0.3
Or see this region's nucleotide sequence