Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt flgM and flgA are separated by 109 nucleotides flgA and OKGIIK_02640 overlap by 4 nucleotides OKGIIK_02640 and OKGIIK_02645 are separated by 195 nucleotides
OKGIIK_02630: flgM - flagellar biosynthesis anti-sigma factor FlgM, at 613,559 to 613,882
flgM
OKGIIK_02635: flgA - flagellar basal body P-ring formation protein FlgA, at 613,992 to 614,699
flgA
OKGIIK_02640: OKGIIK_02640 - Chemotaxis protein, at 614,696 to 616,000
_02640
OKGIIK_02645: OKGIIK_02645 - Chemotaxis protein, at 616,196 to 617,134
_02645
Position (kb)
614
615
616
617 Strain fitness (log2 ratio)
-2
-1
0
1
2 at 613.776 kb on - strand, within flgM at 613.913 kb on - strand at 613.913 kb on - strand at 613.913 kb on - strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.945 kb on + strand at 613.946 kb on - strand at 613.946 kb on - strand at 613.946 kb on - strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.960 kb on + strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.961 kb on - strand at 613.962 kb on + strand at 613.962 kb on + strand at 613.962 kb on + strand at 613.963 kb on - strand at 613.963 kb on - strand at 613.963 kb on - strand at 614.269 kb on + strand, within flgA at 614.269 kb on + strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 614.567 kb on - strand, within flgA at 615.184 kb on + strand, within OKGIIK_02640 at 615.184 kb on + strand, within OKGIIK_02640 at 615.184 kb on + strand, within OKGIIK_02640 at 615.184 kb on + strand, within OKGIIK_02640 at 615.707 kb on - strand, within OKGIIK_02640 at 615.909 kb on + strand at 615.909 kb on + strand at 615.909 kb on + strand at 615.909 kb on + strand at 615.909 kb on + strand at 615.909 kb on + strand at 615.910 kb on - strand at 616.159 kb on - strand at 616.159 kb on - strand at 616.176 kb on + strand at 616.177 kb on - strand at 616.231 kb on + strand at 616.231 kb on + strand at 616.231 kb on + strand at 616.232 kb on - strand at 616.391 kb on + strand, within OKGIIK_02645 at 616.391 kb on + strand, within OKGIIK_02645 at 616.392 kb on - strand, within OKGIIK_02645 at 616.439 kb on + strand, within OKGIIK_02645 at 616.440 kb on - strand, within OKGIIK_02645 at 616.932 kb on - strand, within OKGIIK_02645 at 616.932 kb on - strand, within OKGIIK_02645
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 613,776 - flgM OKGIIK_02630 0.67 -2.0 613,913 - +1.2 613,913 - -0.5 613,913 - -1.1 613,945 + -1.2 613,945 + -0.1 613,945 + -0.4 613,945 + +0.4 613,945 + -1.3 613,946 - +0.2 613,946 - -1.9 613,946 - +0.7 613,960 + +0.0 613,960 + -2.3 613,960 + -0.5 613,960 + +1.3 613,960 + -0.3 613,960 + -0.5 613,960 + -0.1 613,960 + -0.8 613,960 + +0.7 613,960 + -0.8 613,960 + -0.1 613,960 + -0.1 613,960 + +0.9 613,960 + -0.1 613,960 + -0.5 613,960 + +1.1 613,960 + -0.1 613,960 + +0.9 613,960 + +0.3 613,960 + +0.8 613,960 + +0.2 613,961 - +0.5 613,961 - -0.4 613,961 - +1.2 613,961 - +1.7 613,961 - -2.2 613,961 - -0.5 613,961 - -0.5 613,961 - -0.3 613,961 - -0.5 613,961 - +2.1 613,961 - +1.3 613,961 - +0.2 613,961 - -1.0 613,961 - -0.6 613,961 - -0.7 613,961 - -0.1 613,961 - -0.8 613,962 + +1.4 613,962 + +0.7 613,962 + +0.3 613,963 - +0.9 613,963 - -0.5 613,963 - +0.0 614,269 + flgA OKGIIK_02635 0.39 +0.2 614,269 + flgA OKGIIK_02635 0.39 +0.5 614,567 - flgA OKGIIK_02635 0.81 -0.7 614,567 - flgA OKGIIK_02635 0.81 -0.4 614,567 - flgA OKGIIK_02635 0.81 +0.0 614,567 - flgA OKGIIK_02635 0.81 -0.2 614,567 - flgA OKGIIK_02635 0.81 +0.6 615,184 + OKGIIK_02640 0.37 -1.8 615,184 + OKGIIK_02640 0.37 +0.5 615,184 + OKGIIK_02640 0.37 -2.2 615,184 + OKGIIK_02640 0.37 -1.8 615,707 - OKGIIK_02640 0.77 -0.5 615,909 + -0.6 615,909 + -0.1 615,909 + +0.5 615,909 + -0.3 615,909 + -1.3 615,909 + +1.0 615,910 - -1.2 616,159 - -0.5 616,159 - -1.0 616,176 + +1.2 616,177 - -1.9 616,231 + +0.1 616,231 + -0.7 616,231 + -1.8 616,232 - +0.2 616,391 + OKGIIK_02645 0.21 -0.1 616,391 + OKGIIK_02645 0.21 -0.3 616,392 - OKGIIK_02645 0.21 -0.1 616,439 + OKGIIK_02645 0.26 +0.1 616,440 - OKGIIK_02645 0.26 +0.0 616,932 - OKGIIK_02645 0.78 -0.8 616,932 - OKGIIK_02645 0.78 +0.2
Or see this region's nucleotide sequence