Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt hprK and raiA are separated by 196 nucleotides raiA and OKGIIK_02265 are separated by 149 nucleotides
OKGIIK_02255: hprK - HPr(Ser) kinase/phosphatase, at 528,555 to 529,457
hprK
OKGIIK_02260: raiA - ribosomal subunit interface protein, at 529,654 to 529,977
raiA
OKGIIK_02265: OKGIIK_02265 - RNA polymerase factor sigma-54, at 530,127 to 531,623
_02265
Position (kb) 529
530 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 528.820 kb on + strand, within hprK at 528.820 kb on + strand, within hprK at 528.820 kb on + strand, within hprK at 528.820 kb on + strand, within hprK at 528.820 kb on + strand, within hprK at 528.820 kb on + strand, within hprK at 528.821 kb on - strand, within hprK at 528.821 kb on - strand, within hprK at 528.821 kb on - strand, within hprK at 528.821 kb on - strand, within hprK at 528.821 kb on - strand, within hprK at 528.821 kb on - strand, within hprK at 528.993 kb on + strand, within hprK at 528.994 kb on - strand, within hprK at 528.994 kb on - strand, within hprK at 529.120 kb on + strand, within hprK at 529.120 kb on + strand, within hprK at 529.120 kb on + strand, within hprK at 529.120 kb on + strand, within hprK at 529.120 kb on + strand, within hprK at 529.120 kb on + strand, within hprK at 529.121 kb on - strand, within hprK at 529.121 kb on - strand, within hprK at 529.121 kb on - strand, within hprK at 529.129 kb on + strand, within hprK at 529.129 kb on + strand, within hprK at 529.129 kb on + strand, within hprK at 529.129 kb on + strand, within hprK at 529.129 kb on + strand, within hprK at 529.129 kb on + strand, within hprK at 529.129 kb on + strand, within hprK at 529.130 kb on - strand, within hprK at 529.130 kb on - strand, within hprK at 529.130 kb on - strand, within hprK at 529.130 kb on - strand, within hprK at 529.130 kb on - strand, within hprK at 529.130 kb on - strand, within hprK at 529.252 kb on + strand, within hprK at 529.253 kb on - strand, within hprK at 529.253 kb on - strand, within hprK at 529.253 kb on - strand, within hprK at 529.309 kb on + strand, within hprK at 529.309 kb on + strand, within hprK at 529.309 kb on + strand, within hprK at 529.309 kb on + strand, within hprK at 529.309 kb on + strand, within hprK at 529.309 kb on + strand, within hprK at 529.310 kb on - strand, within hprK at 529.310 kb on - strand, within hprK at 529.310 kb on - strand, within hprK at 529.310 kb on - strand, within hprK at 529.310 kb on - strand, within hprK at 529.310 kb on - strand, within hprK at 529.351 kb on + strand, within hprK at 529.352 kb on - strand, within hprK at 529.477 kb on + strand at 529.477 kb on + strand at 529.477 kb on + strand at 529.478 kb on - strand at 529.601 kb on - strand at 529.601 kb on - strand at 529.601 kb on - strand at 529.601 kb on - strand at 529.715 kb on + strand, within raiA at 529.715 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.750 kb on + strand, within raiA at 529.751 kb on - strand, within raiA at 529.751 kb on - strand, within raiA at 529.751 kb on - strand, within raiA at 529.751 kb on - strand, within raiA at 529.751 kb on - strand, within raiA at 529.751 kb on - strand, within raiA at 529.751 kb on - strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.766 kb on + strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.767 kb on - strand, within raiA at 529.881 kb on + strand, within raiA at 529.881 kb on + strand, within raiA at 529.881 kb on + strand, within raiA at 529.882 kb on - strand, within raiA at 530.068 kb on + strand at 530.068 kb on + strand at 530.068 kb on + strand at 530.068 kb on + strand at 530.068 kb on + strand at 530.068 kb on + strand at 530.068 kb on + strand at 530.068 kb on + strand at 530.069 kb on - strand at 530.069 kb on - strand at 530.069 kb on - strand at 530.069 kb on - strand at 530.069 kb on - strand at 530.075 kb on + strand at 530.075 kb on + strand at 530.075 kb on + strand at 530.075 kb on + strand at 530.076 kb on - strand at 530.076 kb on - strand at 530.076 kb on - strand at 530.076 kb on - strand at 530.076 kb on - strand at 530.076 kb on - strand at 530.179 kb on + strand at 530.180 kb on - strand at 530.180 kb on - strand at 530.359 kb on + strand, within OKGIIK_02265 at 530.359 kb on + strand, within OKGIIK_02265 at 530.359 kb on + strand, within OKGIIK_02265 at 530.359 kb on + strand, within OKGIIK_02265 at 530.359 kb on + strand, within OKGIIK_02265 at 530.359 kb on + strand, within OKGIIK_02265 at 530.359 kb on + strand, within OKGIIK_02265 at 530.360 kb on - strand, within OKGIIK_02265 at 530.360 kb on - strand, within OKGIIK_02265 at 530.360 kb on - strand, within OKGIIK_02265 at 530.360 kb on - strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.395 kb on + strand, within OKGIIK_02265 at 530.396 kb on - strand, within OKGIIK_02265 at 530.396 kb on - strand, within OKGIIK_02265 at 530.396 kb on - strand, within OKGIIK_02265 at 530.396 kb on - strand, within OKGIIK_02265 at 530.396 kb on - strand, within OKGIIK_02265 at 530.396 kb on - strand, within OKGIIK_02265 at 530.492 kb on - strand, within OKGIIK_02265 at 530.650 kb on + strand, within OKGIIK_02265 at 530.650 kb on + strand, within OKGIIK_02265 at 530.651 kb on - strand, within OKGIIK_02265 at 530.729 kb on - strand, within OKGIIK_02265 at 530.746 kb on + strand, within OKGIIK_02265 at 530.747 kb on - strand, within OKGIIK_02265
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 528,820 + hprK OKGIIK_02255 0.29 -0.6 528,820 + hprK OKGIIK_02255 0.29 +0.4 528,820 + hprK OKGIIK_02255 0.29 -1.1 528,820 + hprK OKGIIK_02255 0.29 -0.8 528,820 + hprK OKGIIK_02255 0.29 -0.4 528,820 + hprK OKGIIK_02255 0.29 -2.3 528,821 - hprK OKGIIK_02255 0.29 -1.1 528,821 - hprK OKGIIK_02255 0.29 -2.3 528,821 - hprK OKGIIK_02255 0.29 +0.3 528,821 - hprK OKGIIK_02255 0.29 -0.8 528,821 - hprK OKGIIK_02255 0.29 -2.0 528,821 - hprK OKGIIK_02255 0.29 -1.1 528,993 + hprK OKGIIK_02255 0.49 -1.5 528,994 - hprK OKGIIK_02255 0.49 -1.5 528,994 - hprK OKGIIK_02255 0.49 -3.2 529,120 + hprK OKGIIK_02255 0.63 -1.3 529,120 + hprK OKGIIK_02255 0.63 -1.2 529,120 + hprK OKGIIK_02255 0.63 -1.1 529,120 + hprK OKGIIK_02255 0.63 -0.5 529,120 + hprK OKGIIK_02255 0.63 -0.7 529,120 + hprK OKGIIK_02255 0.63 -1.6 529,121 - hprK OKGIIK_02255 0.63 -1.5 529,121 - hprK OKGIIK_02255 0.63 -1.2 529,121 - hprK OKGIIK_02255 0.63 +0.8 529,129 + hprK OKGIIK_02255 0.64 -1.8 529,129 + hprK OKGIIK_02255 0.64 +0.3 529,129 + hprK OKGIIK_02255 0.64 -1.0 529,129 + hprK OKGIIK_02255 0.64 -1.7 529,129 + hprK OKGIIK_02255 0.64 +0.9 529,129 + hprK OKGIIK_02255 0.64 -0.4 529,129 + hprK OKGIIK_02255 0.64 +0.8 529,130 - hprK OKGIIK_02255 0.64 -0.2 529,130 - hprK OKGIIK_02255 0.64 +1.5 529,130 - hprK OKGIIK_02255 0.64 -0.7 529,130 - hprK OKGIIK_02255 0.64 -1.5 529,130 - hprK OKGIIK_02255 0.64 -0.5 529,130 - hprK OKGIIK_02255 0.64 -1.8 529,252 + hprK OKGIIK_02255 0.77 -1.5 529,253 - hprK OKGIIK_02255 0.77 -2.1 529,253 - hprK OKGIIK_02255 0.77 -2.5 529,253 - hprK OKGIIK_02255 0.77 -1.3 529,309 + hprK OKGIIK_02255 0.83 -0.4 529,309 + hprK OKGIIK_02255 0.83 -2.1 529,309 + hprK OKGIIK_02255 0.83 -2.3 529,309 + hprK OKGIIK_02255 0.83 -1.7 529,309 + hprK OKGIIK_02255 0.83 -1.1 529,309 + hprK OKGIIK_02255 0.83 -0.5 529,310 - hprK OKGIIK_02255 0.84 -1.5 529,310 - hprK OKGIIK_02255 0.84 -1.0 529,310 - hprK OKGIIK_02255 0.84 -0.6 529,310 - hprK OKGIIK_02255 0.84 -0.8 529,310 - hprK OKGIIK_02255 0.84 +0.0 529,310 - hprK OKGIIK_02255 0.84 -3.5 529,351 + hprK OKGIIK_02255 0.88 +1.0 529,352 - hprK OKGIIK_02255 0.88 -1.1 529,477 + +0.2 529,477 + -1.7 529,477 + -0.8 529,478 - -3.2 529,601 - +0.3 529,601 - +2.3 529,601 - +0.5 529,601 - -0.4 529,715 + raiA OKGIIK_02260 0.19 -1.2 529,715 + raiA OKGIIK_02260 0.19 -2.4 529,750 + raiA OKGIIK_02260 0.30 -1.8 529,750 + raiA OKGIIK_02260 0.30 -2.1 529,750 + raiA OKGIIK_02260 0.30 -1.1 529,750 + raiA OKGIIK_02260 0.30 -1.0 529,750 + raiA OKGIIK_02260 0.30 -1.1 529,750 + raiA OKGIIK_02260 0.30 -2.6 529,750 + raiA OKGIIK_02260 0.30 -1.8 529,750 + raiA OKGIIK_02260 0.30 -1.7 529,750 + raiA OKGIIK_02260 0.30 -1.8 529,750 + raiA OKGIIK_02260 0.30 -1.1 529,750 + raiA OKGIIK_02260 0.30 -0.1 529,751 - raiA OKGIIK_02260 0.30 -2.4 529,751 - raiA OKGIIK_02260 0.30 -2.5 529,751 - raiA OKGIIK_02260 0.30 -0.3 529,751 - raiA OKGIIK_02260 0.30 -0.8 529,751 - raiA OKGIIK_02260 0.30 -1.8 529,751 - raiA OKGIIK_02260 0.30 -1.8 529,751 - raiA OKGIIK_02260 0.30 -1.1 529,766 + raiA OKGIIK_02260 0.35 +0.1 529,766 + raiA OKGIIK_02260 0.35 -2.0 529,766 + raiA OKGIIK_02260 0.35 -2.1 529,766 + raiA OKGIIK_02260 0.35 -1.7 529,766 + raiA OKGIIK_02260 0.35 -2.1 529,766 + raiA OKGIIK_02260 0.35 -0.4 529,766 + raiA OKGIIK_02260 0.35 -1.7 529,766 + raiA OKGIIK_02260 0.35 -2.7 529,766 + raiA OKGIIK_02260 0.35 -2.5 529,766 + raiA OKGIIK_02260 0.35 -1.1 529,766 + raiA OKGIIK_02260 0.35 -0.6 529,766 + raiA OKGIIK_02260 0.35 -2.7 529,766 + raiA OKGIIK_02260 0.35 -2.4 529,766 + raiA OKGIIK_02260 0.35 -1.1 529,766 + raiA OKGIIK_02260 0.35 -1.7 529,766 + raiA OKGIIK_02260 0.35 -2.1 529,766 + raiA OKGIIK_02260 0.35 -1.3 529,766 + raiA OKGIIK_02260 0.35 +0.2 529,766 + raiA OKGIIK_02260 0.35 -0.3 529,766 + raiA OKGIIK_02260 0.35 -1.8 529,766 + raiA OKGIIK_02260 0.35 -1.3 529,766 + raiA OKGIIK_02260 0.35 -1.0 529,766 + raiA OKGIIK_02260 0.35 -1.5 529,766 + raiA OKGIIK_02260 0.35 -0.5 529,766 + raiA OKGIIK_02260 0.35 -3.4 529,766 + raiA OKGIIK_02260 0.35 -1.1 529,766 + raiA OKGIIK_02260 0.35 -1.1 529,767 - raiA OKGIIK_02260 0.35 -2.5 529,767 - raiA OKGIIK_02260 0.35 -3.7 529,767 - raiA OKGIIK_02260 0.35 -3.2 529,767 - raiA OKGIIK_02260 0.35 +0.5 529,767 - raiA OKGIIK_02260 0.35 -0.8 529,767 - raiA OKGIIK_02260 0.35 -1.1 529,767 - raiA OKGIIK_02260 0.35 -1.2 529,767 - raiA OKGIIK_02260 0.35 -2.7 529,767 - raiA OKGIIK_02260 0.35 -2.0 529,767 - raiA OKGIIK_02260 0.35 -2.3 529,767 - raiA OKGIIK_02260 0.35 -3.3 529,767 - raiA OKGIIK_02260 0.35 -0.8 529,767 - raiA OKGIIK_02260 0.35 -0.5 529,767 - raiA OKGIIK_02260 0.35 -0.5 529,767 - raiA OKGIIK_02260 0.35 -3.2 529,767 - raiA OKGIIK_02260 0.35 -0.8 529,767 - raiA OKGIIK_02260 0.35 -1.7 529,767 - raiA OKGIIK_02260 0.35 -0.7 529,767 - raiA OKGIIK_02260 0.35 -1.7 529,767 - raiA OKGIIK_02260 0.35 -0.2 529,767 - raiA OKGIIK_02260 0.35 -2.6 529,767 - raiA OKGIIK_02260 0.35 -0.5 529,767 - raiA OKGIIK_02260 0.35 -1.1 529,767 - raiA OKGIIK_02260 0.35 -1.8 529,767 - raiA OKGIIK_02260 0.35 -1.5 529,767 - raiA OKGIIK_02260 0.35 -1.3 529,767 - raiA OKGIIK_02260 0.35 -0.5 529,767 - raiA OKGIIK_02260 0.35 -2.2 529,767 - raiA OKGIIK_02260 0.35 -1.7 529,881 + raiA OKGIIK_02260 0.70 -2.2 529,881 + raiA OKGIIK_02260 0.70 -1.6 529,881 + raiA OKGIIK_02260 0.70 -2.2 529,882 - raiA OKGIIK_02260 0.70 +0.5 530,068 + -1.7 530,068 + -0.2 530,068 + -0.5 530,068 + -1.7 530,068 + -0.4 530,068 + +0.2 530,068 + -0.7 530,068 + -1.4 530,069 - -2.2 530,069 - -1.4 530,069 - +0.7 530,069 - -1.7 530,069 - +0.0 530,075 + -1.8 530,075 + -1.1 530,075 + -2.3 530,075 + -1.3 530,076 - -1.4 530,076 - -0.6 530,076 - +0.6 530,076 - -1.6 530,076 - +0.8 530,076 - +0.5 530,179 + -0.8 530,180 - +0.3 530,180 - -2.5 530,359 + OKGIIK_02265 0.15 +0.0 530,359 + OKGIIK_02265 0.15 +0.2 530,359 + OKGIIK_02265 0.15 -0.3 530,359 + OKGIIK_02265 0.15 +0.5 530,359 + OKGIIK_02265 0.15 -0.7 530,359 + OKGIIK_02265 0.15 +2.0 530,359 + OKGIIK_02265 0.15 -1.5 530,360 - OKGIIK_02265 0.16 +1.1 530,360 - OKGIIK_02265 0.16 -1.8 530,360 - OKGIIK_02265 0.16 +0.1 530,360 - OKGIIK_02265 0.16 +0.7 530,395 + OKGIIK_02265 0.18 -0.2 530,395 + OKGIIK_02265 0.18 +0.8 530,395 + OKGIIK_02265 0.18 -2.2 530,395 + OKGIIK_02265 0.18 -1.1 530,395 + OKGIIK_02265 0.18 -0.8 530,395 + OKGIIK_02265 0.18 +0.9 530,395 + OKGIIK_02265 0.18 -1.1 530,395 + OKGIIK_02265 0.18 -2.4 530,395 + OKGIIK_02265 0.18 -0.5 530,395 + OKGIIK_02265 0.18 -0.1 530,396 - OKGIIK_02265 0.18 +0.1 530,396 - OKGIIK_02265 0.18 +0.4 530,396 - OKGIIK_02265 0.18 -0.1 530,396 - OKGIIK_02265 0.18 -0.8 530,396 - OKGIIK_02265 0.18 -0.3 530,396 - OKGIIK_02265 0.18 -0.5 530,492 - OKGIIK_02265 0.24 -0.2 530,650 + OKGIIK_02265 0.35 +0.5 530,650 + OKGIIK_02265 0.35 +1.1 530,651 - OKGIIK_02265 0.35 -1.0 530,729 - OKGIIK_02265 0.40 +0.2 530,746 + OKGIIK_02265 0.41 -0.1 530,747 - OKGIIK_02265 0.41 +0.1
Or see this region's nucleotide sequence