Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt ruvC and ruvA are separated by 25 nucleotides ruvA and kup are separated by 2 nucleotides kup and ruvB are separated by 118 nucleotides
OKGIIK_01835: ruvC - crossover junction endodeoxyribonuclease RuvC, at 426,446 to 426,967
ruvC
OKGIIK_01840: ruvA - Holliday junction branch migration protein RuvA, at 426,993 to 427,589
ruvA
OKGIIK_01845: kup - potassium uptake protein, at 427,592 to 429,493
kup
OKGIIK_01850: ruvB - Holliday junction branch migration DNA helicase RuvB, at 429,612 to 430,661
ruvB
Position (kb) 427
428
429
430 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 427.098 kb on + strand, within ruvA at 427.173 kb on + strand, within ruvA at 427.706 kb on + strand at 427.707 kb on + strand at 427.817 kb on + strand, within kup at 427.817 kb on + strand, within kup at 427.818 kb on - strand, within kup at 427.818 kb on - strand, within kup at 427.818 kb on - strand, within kup at 428.045 kb on + strand, within kup at 428.045 kb on + strand, within kup at 428.046 kb on - strand, within kup at 428.207 kb on + strand, within kup at 428.397 kb on - strand, within kup at 428.453 kb on + strand, within kup at 428.453 kb on + strand, within kup at 429.319 kb on + strand at 429.319 kb on + strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.505 kb on - strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.541 kb on + strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.542 kb on - strand at 429.543 kb on + strand at 429.543 kb on + strand at 429.544 kb on - strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.578 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 429.584 kb on + strand at 430.159 kb on - strand, within ruvB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 427,098 + ruvA OKGIIK_01840 0.18 +0.5 427,173 + ruvA OKGIIK_01840 0.30 -0.8 427,706 + -1.1 427,707 + -1.9 427,817 + kup OKGIIK_01845 0.12 -1.1 427,817 + kup OKGIIK_01845 0.12 -0.5 427,818 - kup OKGIIK_01845 0.12 -1.5 427,818 - kup OKGIIK_01845 0.12 -0.5 427,818 - kup OKGIIK_01845 0.12 -0.5 428,045 + kup OKGIIK_01845 0.24 -1.3 428,045 + kup OKGIIK_01845 0.24 +1.1 428,046 - kup OKGIIK_01845 0.24 -0.8 428,207 + kup OKGIIK_01845 0.32 -0.5 428,397 - kup OKGIIK_01845 0.42 -1.5 428,453 + kup OKGIIK_01845 0.45 -1.3 428,453 + kup OKGIIK_01845 0.45 -2.1 429,319 + -0.5 429,319 + -1.5 429,505 - -0.7 429,505 - -0.8 429,505 - -1.5 429,505 - +0.0 429,505 - +0.3 429,505 - +0.4 429,541 + -0.6 429,541 + -1.1 429,541 + -0.5 429,541 + -1.1 429,541 + -0.1 429,541 + +0.1 429,541 + +0.9 429,541 + -0.6 429,541 + +0.5 429,541 + -0.1 429,541 + +0.1 429,541 + -0.8 429,541 + -1.3 429,541 + +0.3 429,541 + +1.1 429,542 - +0.3 429,542 - +1.5 429,542 - +0.9 429,542 - -0.7 429,542 - -0.5 429,542 - -0.1 429,542 - -2.4 429,542 - -2.6 429,542 - +0.2 429,542 - +0.2 429,542 - -0.5 429,542 - -0.8 429,542 - +1.0 429,542 - -0.1 429,542 - +0.5 429,543 + +1.2 429,543 + -0.8 429,544 - -3.3 429,578 + -0.8 429,578 + -0.3 429,578 + -2.2 429,578 + -0.8 429,584 + -0.5 429,584 + +2.0 429,584 + -2.2 429,584 + -1.1 429,584 + -0.2 429,584 + -2.5 429,584 + -0.7 429,584 + -1.1 429,584 + -1.1 429,584 + -0.2 429,584 + +0.2 429,584 + +0.2 429,584 + +1.0 429,584 + -1.5 429,584 + +0.8 429,584 + +0.3 429,584 + +0.5 429,584 + -0.3 429,584 + -0.1 429,584 + -0.5 429,584 + +0.3 429,584 + -0.2 430,159 - ruvB OKGIIK_01850 0.52 +1.1
Or see this region's nucleotide sequence