Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01300 and gcvT are separated by 55 nucleotides gcvT and gcvH are separated by 91 nucleotides
OKGIIK_01300: OKGIIK_01300 - Multifunctional fusion protein, at 286,656 to 288,530
_01300
OKGIIK_01305: gcvT - glycine cleavage system aminomethyltransferase GcvT, at 288,586 to 289,719
gcvT
OKGIIK_01310: gcvH - glycine cleavage system protein GcvH, at 289,811 to 290,206
gcvH
Position (kb) 288
289
290 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 287.673 kb on + strand, within OKGIIK_01300 at 287.793 kb on + strand, within OKGIIK_01300 at 287.793 kb on + strand, within OKGIIK_01300 at 287.793 kb on + strand, within OKGIIK_01300 at 287.794 kb on - strand, within OKGIIK_01300 at 287.794 kb on - strand, within OKGIIK_01300 at 287.794 kb on - strand, within OKGIIK_01300 at 287.794 kb on - strand, within OKGIIK_01300 at 287.794 kb on - strand, within OKGIIK_01300 at 287.835 kb on + strand, within OKGIIK_01300 at 287.836 kb on - strand, within OKGIIK_01300 at 287.836 kb on - strand, within OKGIIK_01300 at 287.836 kb on - strand, within OKGIIK_01300 at 287.836 kb on - strand, within OKGIIK_01300 at 287.836 kb on - strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.096 kb on + strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.097 kb on - strand, within OKGIIK_01300 at 288.249 kb on + strand, within OKGIIK_01300 at 288.360 kb on + strand at 288.414 kb on + strand at 288.415 kb on - strand at 288.415 kb on - strand at 288.415 kb on - strand at 288.584 kb on + strand at 288.584 kb on + strand at 288.585 kb on - strand at 288.635 kb on + strand at 288.706 kb on + strand, within gcvT at 288.707 kb on - strand, within gcvT at 288.872 kb on - strand, within gcvT at 288.922 kb on + strand, within gcvT at 288.923 kb on - strand, within gcvT at 288.923 kb on - strand, within gcvT at 289.198 kb on + strand, within gcvT at 289.199 kb on - strand, within gcvT at 289.679 kb on - strand at 289.681 kb on - strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.762 kb on + strand at 289.763 kb on - strand at 289.763 kb on - strand at 289.763 kb on - strand at 289.777 kb on + strand at 289.934 kb on + strand, within gcvH at 289.935 kb on - strand, within gcvH at 290.021 kb on + strand, within gcvH at 290.021 kb on + strand, within gcvH at 290.021 kb on + strand, within gcvH at 290.175 kb on - strand at 290.175 kb on - strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.385 kb on + strand at 290.386 kb on - strand at 290.386 kb on - strand at 290.386 kb on - strand at 290.386 kb on - strand at 290.690 kb on + strand at 290.702 kb on + strand at 290.718 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 287,673 + OKGIIK_01300 0.54 -0.2 287,793 + OKGIIK_01300 0.61 -1.8 287,793 + OKGIIK_01300 0.61 -0.5 287,793 + OKGIIK_01300 0.61 -1.9 287,794 - OKGIIK_01300 0.61 -0.2 287,794 - OKGIIK_01300 0.61 -0.8 287,794 - OKGIIK_01300 0.61 -0.6 287,794 - OKGIIK_01300 0.61 -0.1 287,794 - OKGIIK_01300 0.61 -2.7 287,835 + OKGIIK_01300 0.63 -0.4 287,836 - OKGIIK_01300 0.63 -1.3 287,836 - OKGIIK_01300 0.63 +0.8 287,836 - OKGIIK_01300 0.63 -2.5 287,836 - OKGIIK_01300 0.63 -0.7 287,836 - OKGIIK_01300 0.63 -0.9 288,096 + OKGIIK_01300 0.77 +1.8 288,096 + OKGIIK_01300 0.77 -1.1 288,096 + OKGIIK_01300 0.77 +0.3 288,096 + OKGIIK_01300 0.77 -0.3 288,096 + OKGIIK_01300 0.77 -1.1 288,096 + OKGIIK_01300 0.77 +0.1 288,096 + OKGIIK_01300 0.77 +0.9 288,096 + OKGIIK_01300 0.77 -2.5 288,096 + OKGIIK_01300 0.77 -0.3 288,096 + OKGIIK_01300 0.77 +0.8 288,097 - OKGIIK_01300 0.77 -0.6 288,097 - OKGIIK_01300 0.77 +0.1 288,097 - OKGIIK_01300 0.77 +0.1 288,097 - OKGIIK_01300 0.77 -0.1 288,097 - OKGIIK_01300 0.77 +0.3 288,097 - OKGIIK_01300 0.77 -0.1 288,097 - OKGIIK_01300 0.77 -1.1 288,097 - OKGIIK_01300 0.77 -1.4 288,097 - OKGIIK_01300 0.77 -0.1 288,097 - OKGIIK_01300 0.77 +0.8 288,097 - OKGIIK_01300 0.77 +0.5 288,097 - OKGIIK_01300 0.77 -0.2 288,097 - OKGIIK_01300 0.77 -0.9 288,249 + OKGIIK_01300 0.85 -0.7 288,360 + +0.6 288,414 + +0.4 288,415 - -0.8 288,415 - +0.0 288,415 - +0.8 288,584 + +0.4 288,584 + +0.3 288,585 - +0.3 288,635 + -0.0 288,706 + gcvT OKGIIK_01305 0.11 -1.1 288,707 - gcvT OKGIIK_01305 0.11 -0.8 288,872 - gcvT OKGIIK_01305 0.25 -0.1 288,922 + gcvT OKGIIK_01305 0.30 +0.2 288,923 - gcvT OKGIIK_01305 0.30 -1.8 288,923 - gcvT OKGIIK_01305 0.30 -1.1 289,198 + gcvT OKGIIK_01305 0.54 -2.1 289,199 - gcvT OKGIIK_01305 0.54 +0.1 289,679 - -1.8 289,681 - -0.8 289,762 + -2.0 289,762 + +0.9 289,762 + -0.8 289,762 + -0.5 289,762 + -1.3 289,762 + +0.8 289,762 + -0.1 289,762 + -1.0 289,763 - +1.3 289,763 - +0.8 289,763 - -0.2 289,777 + -0.3 289,934 + gcvH OKGIIK_01310 0.31 -0.6 289,935 - gcvH OKGIIK_01310 0.31 -1.5 290,021 + gcvH OKGIIK_01310 0.53 -1.6 290,021 + gcvH OKGIIK_01310 0.53 -2.7 290,021 + gcvH OKGIIK_01310 0.53 -1.8 290,175 - -1.6 290,175 - +0.1 290,385 + +0.3 290,385 + -2.9 290,385 + -0.5 290,385 + -0.6 290,385 + -0.2 290,385 + +0.4 290,385 + -0.8 290,385 + -0.8 290,385 + -0.5 290,385 + +1.3 290,385 + -0.6 290,385 + +1.2 290,385 + -0.8 290,385 + -0.5 290,385 + -1.3 290,386 - +0.5 290,386 - +0.5 290,386 - -1.6 290,386 - +0.1 290,690 + +1.2 290,702 + +0.2 290,718 + +0.2
Or see this region's nucleotide sequence