Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt rfbD and rfbC are separated by 143 nucleotides rfbC and rfbA overlap by 4 nucleotides rfbA and rfbB overlap by 4 nucleotides
OKGIIK_01160: rfbD - dTDP-4-dehydrorhamnose reductase, at 250,252 to 251,187
rfbD
OKGIIK_01165: rfbC - dTDP-4-dehydrorhamnose 3,5-epimerase, at 251,331 to 251,888
rfbC
OKGIIK_01170: rfbA - glucose-1-phosphate thymidylyltransferase RfbA, at 251,885 to 252,769
rfbA
OKGIIK_01175: rfbB - dTDP-glucose 4,6-dehydratase, at 252,766 to 253,836
rfbB
Position (kb) 251
252
253 Strain fitness (log2 ratio) -3
-2
-1
0
1
2 at 250.973 kb on + strand, within rfbD at 250.974 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.204 kb on - strand at 251.333 kb on - strand at 251.333 kb on - strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.341 kb on + strand at 251.342 kb on - strand at 251.482 kb on + strand, within rfbC at 251.482 kb on + strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.483 kb on - strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.500 kb on + strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.501 kb on - strand, within rfbC at 251.633 kb on + strand, within rfbC at 251.650 kb on + strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.651 kb on - strand, within rfbC at 251.705 kb on - strand, within rfbC at 251.754 kb on + strand, within rfbC at 251.754 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.800 kb on + strand, within rfbC at 251.801 kb on - strand, within rfbC at 251.809 kb on + strand, within rfbC at 251.923 kb on - strand at 251.923 kb on - strand at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.057 kb on + strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.058 kb on - strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.153 kb on + strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.154 kb on - strand, within rfbA at 252.216 kb on + strand, within rfbA at 252.217 kb on - strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.243 kb on + strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.244 kb on - strand, within rfbA at 252.261 kb on + strand, within rfbA at 252.261 kb on + strand, within rfbA at 252.262 kb on - strand, within rfbA at 252.262 kb on - strand, within rfbA at 252.429 kb on + strand, within rfbA at 252.535 kb on - strand, within rfbA at 252.535 kb on - strand, within rfbA at 252.657 kb on + strand, within rfbA at 252.657 kb on + strand, within rfbA at 252.657 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.660 kb on + strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.661 kb on - strand, within rfbA at 252.678 kb on + strand, within rfbA at 252.678 kb on + strand, within rfbA at 252.679 kb on - strand, within rfbA at 252.792 kb on - strand at 252.792 kb on - strand at 252.792 kb on - strand at 252.792 kb on - strand at 252.836 kb on + strand at 252.836 kb on + strand at 252.836 kb on + strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.837 kb on - strand at 252.904 kb on + strand, within rfbB at 252.905 kb on - strand, within rfbB at 252.905 kb on - strand, within rfbB at 252.905 kb on - strand, within rfbB at 252.905 kb on - strand, within rfbB at 252.905 kb on - strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.959 kb on + strand, within rfbB at 252.960 kb on - strand, within rfbB at 252.960 kb on - strand, within rfbB at 252.960 kb on - strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.076 kb on + strand, within rfbB at 253.077 kb on - strand, within rfbB at 253.077 kb on - strand, within rfbB at 253.077 kb on - strand, within rfbB at 253.077 kb on - strand, within rfbB at 253.242 kb on - strand, within rfbB at 253.250 kb on + strand, within rfbB at 253.250 kb on + strand, within rfbB at 253.250 kb on + strand, within rfbB at 253.250 kb on + strand, within rfbB at 253.250 kb on + strand, within rfbB at 253.251 kb on - strand, within rfbB at 253.251 kb on - strand, within rfbB at 253.251 kb on - strand, within rfbB at 253.289 kb on + strand, within rfbB at 253.289 kb on + strand, within rfbB at 253.289 kb on + strand, within rfbB at 253.289 kb on + strand, within rfbB at 253.289 kb on + strand, within rfbB at 253.289 kb on + strand, within rfbB at 253.290 kb on - strand, within rfbB at 253.290 kb on - strand, within rfbB at 253.290 kb on - strand, within rfbB at 253.290 kb on - strand, within rfbB at 253.293 kb on + strand, within rfbB at 253.293 kb on + strand, within rfbB at 253.293 kb on + strand, within rfbB at 253.293 kb on + strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.294 kb on - strand, within rfbB at 253.406 kb on + strand, within rfbB at 253.406 kb on + strand, within rfbB at 253.406 kb on + strand, within rfbB at 253.406 kb on + strand, within rfbB at 253.407 kb on - strand, within rfbB at 253.407 kb on - strand, within rfbB at 253.407 kb on - strand, within rfbB at 253.407 kb on - strand, within rfbB at 253.407 kb on - strand, within rfbB at 253.476 kb on - strand, within rfbB at 253.709 kb on + strand, within rfbB at 253.709 kb on + strand, within rfbB at 253.710 kb on - strand, within rfbB at 253.710 kb on - strand, within rfbB at 253.710 kb on - strand, within rfbB
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 250,973 + rfbD OKGIIK_01160 0.77 -0.8 250,974 - rfbD OKGIIK_01160 0.77 -0.5 250,974 - rfbD OKGIIK_01160 0.77 -0.5 251,176 + -0.2 251,176 + +1.2 251,176 + +0.3 251,176 + +0.7 251,177 - -1.1 251,177 - +1.8 251,177 - +1.4 251,177 - -0.5 251,177 - -0.5 251,177 - -0.5 251,203 + -0.5 251,203 + +0.1 251,203 + -1.6 251,204 - -0.3 251,333 - -1.1 251,333 - -0.1 251,341 + +0.7 251,341 + +0.5 251,341 + +0.0 251,341 + +1.5 251,342 - -0.3 251,482 + rfbC OKGIIK_01165 0.27 -1.1 251,482 + rfbC OKGIIK_01165 0.27 -1.1 251,483 - rfbC OKGIIK_01165 0.27 -1.1 251,483 - rfbC OKGIIK_01165 0.27 -1.5 251,500 + rfbC OKGIIK_01165 0.30 -1.6 251,500 + rfbC OKGIIK_01165 0.30 -0.8 251,500 + rfbC OKGIIK_01165 0.30 -2.1 251,501 - rfbC OKGIIK_01165 0.30 -1.1 251,501 - rfbC OKGIIK_01165 0.30 -2.2 251,633 + rfbC OKGIIK_01165 0.54 -0.4 251,650 + rfbC OKGIIK_01165 0.57 -1.3 251,651 - rfbC OKGIIK_01165 0.57 -1.5 251,651 - rfbC OKGIIK_01165 0.57 -0.8 251,705 - rfbC OKGIIK_01165 0.67 -0.5 251,754 + rfbC OKGIIK_01165 0.76 -0.5 251,754 + rfbC OKGIIK_01165 0.76 -1.4 251,800 + rfbC OKGIIK_01165 0.84 -1.8 251,800 + rfbC OKGIIK_01165 0.84 -2.6 251,801 - rfbC OKGIIK_01165 0.84 -0.1 251,809 + rfbC OKGIIK_01165 0.86 -3.4 251,923 - -1.6 251,923 - +0.1 252,057 + rfbA OKGIIK_01170 0.19 -1.1 252,057 + rfbA OKGIIK_01170 0.19 -0.5 252,057 + rfbA OKGIIK_01170 0.19 -1.1 252,057 + rfbA OKGIIK_01170 0.19 -0.8 252,058 - rfbA OKGIIK_01170 0.20 -0.8 252,058 - rfbA OKGIIK_01170 0.20 -1.1 252,058 - rfbA OKGIIK_01170 0.20 -0.6 252,058 - rfbA OKGIIK_01170 0.20 -0.8 252,058 - rfbA OKGIIK_01170 0.20 -1.1 252,153 + rfbA OKGIIK_01170 0.30 -0.5 252,153 + rfbA OKGIIK_01170 0.30 +0.1 252,153 + rfbA OKGIIK_01170 0.30 -2.1 252,154 - rfbA OKGIIK_01170 0.30 -2.6 252,154 - rfbA OKGIIK_01170 0.30 -1.9 252,154 - rfbA OKGIIK_01170 0.30 -0.4 252,216 + rfbA OKGIIK_01170 0.37 -0.6 252,217 - rfbA OKGIIK_01170 0.38 -1.3 252,243 + rfbA OKGIIK_01170 0.40 -1.8 252,243 + rfbA OKGIIK_01170 0.40 -2.0 252,243 + rfbA OKGIIK_01170 0.40 +1.2 252,243 + rfbA OKGIIK_01170 0.40 -2.2 252,243 + rfbA OKGIIK_01170 0.40 -2.3 252,244 - rfbA OKGIIK_01170 0.41 -0.9 252,244 - rfbA OKGIIK_01170 0.41 -0.6 252,244 - rfbA OKGIIK_01170 0.41 -0.5 252,244 - rfbA OKGIIK_01170 0.41 -1.1 252,244 - rfbA OKGIIK_01170 0.41 +0.3 252,244 - rfbA OKGIIK_01170 0.41 +0.8 252,244 - rfbA OKGIIK_01170 0.41 -0.7 252,261 + rfbA OKGIIK_01170 0.42 -1.4 252,261 + rfbA OKGIIK_01170 0.42 -1.8 252,262 - rfbA OKGIIK_01170 0.43 -1.1 252,262 - rfbA OKGIIK_01170 0.43 -0.8 252,429 + rfbA OKGIIK_01170 0.61 -1.2 252,535 - rfbA OKGIIK_01170 0.73 -0.8 252,535 - rfbA OKGIIK_01170 0.73 -3.1 252,657 + rfbA OKGIIK_01170 0.87 -0.8 252,657 + rfbA OKGIIK_01170 0.87 -1.1 252,657 + rfbA OKGIIK_01170 0.87 +1.1 252,660 + rfbA OKGIIK_01170 0.88 -0.6 252,660 + rfbA OKGIIK_01170 0.88 -0.5 252,660 + rfbA OKGIIK_01170 0.88 -1.6 252,660 + rfbA OKGIIK_01170 0.88 -1.6 252,660 + rfbA OKGIIK_01170 0.88 -1.8 252,660 + rfbA OKGIIK_01170 0.88 -1.6 252,660 + rfbA OKGIIK_01170 0.88 +0.5 252,660 + rfbA OKGIIK_01170 0.88 -0.5 252,661 - rfbA OKGIIK_01170 0.88 -2.9 252,661 - rfbA OKGIIK_01170 0.88 -2.2 252,661 - rfbA OKGIIK_01170 0.88 -0.3 252,661 - rfbA OKGIIK_01170 0.88 +1.5 252,661 - rfbA OKGIIK_01170 0.88 -0.5 252,661 - rfbA OKGIIK_01170 0.88 -0.8 252,661 - rfbA OKGIIK_01170 0.88 +0.4 252,661 - rfbA OKGIIK_01170 0.88 -0.3 252,661 - rfbA OKGIIK_01170 0.88 -0.5 252,661 - rfbA OKGIIK_01170 0.88 -1.3 252,678 + rfbA OKGIIK_01170 0.90 -0.2 252,678 + rfbA OKGIIK_01170 0.90 -0.5 252,679 - rfbA OKGIIK_01170 0.90 +0.6 252,792 - +0.3 252,792 - -0.4 252,792 - +0.3 252,792 - +0.8 252,836 + -0.9 252,836 + -1.2 252,836 + -1.6 252,837 - -2.3 252,837 - -1.8 252,837 - -1.6 252,837 - -2.6 252,837 - -1.7 252,904 + rfbB OKGIIK_01175 0.13 +0.5 252,905 - rfbB OKGIIK_01175 0.13 +1.1 252,905 - rfbB OKGIIK_01175 0.13 -2.1 252,905 - rfbB OKGIIK_01175 0.13 -1.1 252,905 - rfbB OKGIIK_01175 0.13 -1.5 252,905 - rfbB OKGIIK_01175 0.13 -0.8 252,959 + rfbB OKGIIK_01175 0.18 -0.2 252,959 + rfbB OKGIIK_01175 0.18 -1.1 252,959 + rfbB OKGIIK_01175 0.18 -0.1 252,959 + rfbB OKGIIK_01175 0.18 -0.5 252,959 + rfbB OKGIIK_01175 0.18 -0.7 252,959 + rfbB OKGIIK_01175 0.18 -2.5 252,959 + rfbB OKGIIK_01175 0.18 -0.2 252,960 - rfbB OKGIIK_01175 0.18 -0.8 252,960 - rfbB OKGIIK_01175 0.18 -2.4 252,960 - rfbB OKGIIK_01175 0.18 -0.3 253,076 + rfbB OKGIIK_01175 0.29 -0.1 253,076 + rfbB OKGIIK_01175 0.29 -1.8 253,076 + rfbB OKGIIK_01175 0.29 -0.6 253,076 + rfbB OKGIIK_01175 0.29 -1.3 253,076 + rfbB OKGIIK_01175 0.29 -1.1 253,076 + rfbB OKGIIK_01175 0.29 +0.5 253,076 + rfbB OKGIIK_01175 0.29 -1.5 253,076 + rfbB OKGIIK_01175 0.29 +0.9 253,077 - rfbB OKGIIK_01175 0.29 -0.9 253,077 - rfbB OKGIIK_01175 0.29 -2.6 253,077 - rfbB OKGIIK_01175 0.29 -0.1 253,077 - rfbB OKGIIK_01175 0.29 +0.5 253,242 - rfbB OKGIIK_01175 0.44 -0.3 253,250 + rfbB OKGIIK_01175 0.45 -0.1 253,250 + rfbB OKGIIK_01175 0.45 -1.3 253,250 + rfbB OKGIIK_01175 0.45 -1.2 253,250 + rfbB OKGIIK_01175 0.45 -1.3 253,250 + rfbB OKGIIK_01175 0.45 -1.5 253,251 - rfbB OKGIIK_01175 0.45 +0.5 253,251 - rfbB OKGIIK_01175 0.45 -1.0 253,251 - rfbB OKGIIK_01175 0.45 -1.0 253,289 + rfbB OKGIIK_01175 0.49 -2.4 253,289 + rfbB OKGIIK_01175 0.49 -0.1 253,289 + rfbB OKGIIK_01175 0.49 +0.2 253,289 + rfbB OKGIIK_01175 0.49 -0.7 253,289 + rfbB OKGIIK_01175 0.49 -2.5 253,289 + rfbB OKGIIK_01175 0.49 -0.6 253,290 - rfbB OKGIIK_01175 0.49 -1.6 253,290 - rfbB OKGIIK_01175 0.49 -0.1 253,290 - rfbB OKGIIK_01175 0.49 -1.8 253,290 - rfbB OKGIIK_01175 0.49 -1.4 253,293 + rfbB OKGIIK_01175 0.49 -1.4 253,293 + rfbB OKGIIK_01175 0.49 -2.4 253,293 + rfbB OKGIIK_01175 0.49 -0.5 253,293 + rfbB OKGIIK_01175 0.49 -2.4 253,294 - rfbB OKGIIK_01175 0.49 -1.9 253,294 - rfbB OKGIIK_01175 0.49 -1.5 253,294 - rfbB OKGIIK_01175 0.49 -0.5 253,294 - rfbB OKGIIK_01175 0.49 -0.5 253,294 - rfbB OKGIIK_01175 0.49 +0.9 253,294 - rfbB OKGIIK_01175 0.49 -2.7 253,294 - rfbB OKGIIK_01175 0.49 +0.8 253,294 - rfbB OKGIIK_01175 0.49 -0.3 253,294 - rfbB OKGIIK_01175 0.49 -1.5 253,406 + rfbB OKGIIK_01175 0.60 -1.1 253,406 + rfbB OKGIIK_01175 0.60 -0.9 253,406 + rfbB OKGIIK_01175 0.60 -0.8 253,406 + rfbB OKGIIK_01175 0.60 +0.1 253,407 - rfbB OKGIIK_01175 0.60 -0.5 253,407 - rfbB OKGIIK_01175 0.60 -0.6 253,407 - rfbB OKGIIK_01175 0.60 -0.5 253,407 - rfbB OKGIIK_01175 0.60 -0.1 253,407 - rfbB OKGIIK_01175 0.60 -1.1 253,476 - rfbB OKGIIK_01175 0.66 -2.0 253,709 + rfbB OKGIIK_01175 0.88 -0.1 253,709 + rfbB OKGIIK_01175 0.88 -0.8 253,710 - rfbB OKGIIK_01175 0.88 -1.9 253,710 - rfbB OKGIIK_01175 0.88 -1.6 253,710 - rfbB OKGIIK_01175 0.88 -0.7
Or see this region's nucleotide sequence