Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_01150 and cpsB are separated by 412 nucleotides cpsB and rfbD are separated by 16 nucleotides
OKGIIK_01150: OKGIIK_01150 - Protein adenylyltransferase, at 247,277 to 248,410
_01150
OKGIIK_01155: cpsB - mannose-1-phosphate guanylyltransferase/mannose-6-phosphate isomerase, at 248,823 to 250,235
cpsB
OKGIIK_01160: rfbD - dTDP-4-dehydrorhamnose reductase, at 250,252 to 251,187
rfbD
Position (kb) 248
249
250
251 Strain fitness (log2 ratio) -4
-3
-2
-1
0
1
2 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.938 kb on + strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.939 kb on - strand, within OKGIIK_01150 at 247.956 kb on + strand, within OKGIIK_01150 at 247.956 kb on + strand, within OKGIIK_01150 at 247.956 kb on + strand, within OKGIIK_01150 at 247.956 kb on + strand, within OKGIIK_01150 at 247.956 kb on + strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 247.957 kb on - strand, within OKGIIK_01150 at 248.213 kb on + strand, within OKGIIK_01150 at 248.214 kb on - strand, within OKGIIK_01150 at 248.283 kb on + strand, within OKGIIK_01150 at 248.284 kb on - strand, within OKGIIK_01150 at 248.284 kb on - strand, within OKGIIK_01150 at 248.413 kb on + strand at 248.413 kb on + strand at 248.413 kb on + strand at 248.698 kb on - strand at 248.794 kb on + strand at 248.795 kb on - strand at 248.795 kb on - strand at 248.795 kb on - strand at 248.836 kb on + strand at 248.837 kb on - strand at 248.837 kb on - strand at 248.875 kb on + strand at 248.875 kb on + strand at 248.876 kb on - strand at 248.876 kb on - strand at 248.954 kb on + strand at 248.955 kb on - strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.956 kb on + strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 248.957 kb on - strand at 249.133 kb on + strand, within cpsB at 249.133 kb on + strand, within cpsB at 249.133 kb on + strand, within cpsB at 249.134 kb on - strand, within cpsB at 249.134 kb on - strand, within cpsB at 249.134 kb on - strand, within cpsB at 249.134 kb on - strand, within cpsB at 249.568 kb on + strand, within cpsB at 249.626 kb on - strand, within cpsB at 249.667 kb on + strand, within cpsB at 249.685 kb on + strand, within cpsB at 249.686 kb on - strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.772 kb on + strand, within cpsB at 249.773 kb on - strand, within cpsB at 249.906 kb on + strand, within cpsB at 249.907 kb on - strand, within cpsB at 249.907 kb on - strand, within cpsB at 249.907 kb on - strand, within cpsB at 250.268 kb on - strand at 250.348 kb on - strand, within rfbD at 250.781 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.817 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.847 kb on + strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.848 kb on - strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.859 kb on + strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.860 kb on - strand, within rfbD at 250.973 kb on + strand, within rfbD at 250.974 kb on - strand, within rfbD at 250.974 kb on - strand, within rfbD at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.176 kb on + strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.177 kb on - strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.203 kb on + strand at 251.204 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 247,938 + OKGIIK_01150 0.58 -1.2 247,938 + OKGIIK_01150 0.58 -0.2 247,938 + OKGIIK_01150 0.58 -1.0 247,938 + OKGIIK_01150 0.58 +0.4 247,938 + OKGIIK_01150 0.58 +0.3 247,938 + OKGIIK_01150 0.58 +1.1 247,938 + OKGIIK_01150 0.58 -1.5 247,938 + OKGIIK_01150 0.58 +0.3 247,938 + OKGIIK_01150 0.58 -1.3 247,938 + OKGIIK_01150 0.58 -0.2 247,939 - OKGIIK_01150 0.58 -1.2 247,939 - OKGIIK_01150 0.58 -0.0 247,939 - OKGIIK_01150 0.58 -0.6 247,939 - OKGIIK_01150 0.58 -0.3 247,939 - OKGIIK_01150 0.58 -0.5 247,939 - OKGIIK_01150 0.58 +0.5 247,939 - OKGIIK_01150 0.58 +0.5 247,939 - OKGIIK_01150 0.58 +0.4 247,939 - OKGIIK_01150 0.58 -0.5 247,939 - OKGIIK_01150 0.58 -0.7 247,939 - OKGIIK_01150 0.58 +0.2 247,939 - OKGIIK_01150 0.58 -0.3 247,939 - OKGIIK_01150 0.58 -0.8 247,939 - OKGIIK_01150 0.58 +0.6 247,939 - OKGIIK_01150 0.58 -1.4 247,956 + OKGIIK_01150 0.60 -2.4 247,956 + OKGIIK_01150 0.60 +0.2 247,956 + OKGIIK_01150 0.60 -0.8 247,956 + OKGIIK_01150 0.60 +0.5 247,956 + OKGIIK_01150 0.60 +0.6 247,957 - OKGIIK_01150 0.60 -0.4 247,957 - OKGIIK_01150 0.60 -2.1 247,957 - OKGIIK_01150 0.60 +0.1 247,957 - OKGIIK_01150 0.60 +0.4 247,957 - OKGIIK_01150 0.60 -0.6 247,957 - OKGIIK_01150 0.60 -0.6 247,957 - OKGIIK_01150 0.60 -0.7 247,957 - OKGIIK_01150 0.60 -0.4 247,957 - OKGIIK_01150 0.60 +0.9 247,957 - OKGIIK_01150 0.60 -1.3 247,957 - OKGIIK_01150 0.60 +0.2 247,957 - OKGIIK_01150 0.60 -0.6 248,213 + OKGIIK_01150 0.83 +0.1 248,214 - OKGIIK_01150 0.83 +0.4 248,283 + OKGIIK_01150 0.89 +0.7 248,284 - OKGIIK_01150 0.89 -1.5 248,284 - OKGIIK_01150 0.89 -0.1 248,413 + +2.1 248,413 + +1.3 248,413 + -1.5 248,698 - +0.5 248,794 + -0.9 248,795 - -0.6 248,795 - -1.8 248,795 - -0.4 248,836 + -0.5 248,837 - -0.8 248,837 - -0.8 248,875 + -2.6 248,875 + -2.3 248,876 - -3.0 248,876 - -0.5 248,954 + -0.8 248,955 - -1.1 248,956 + -0.5 248,956 + +0.8 248,956 + -0.8 248,956 + -1.1 248,956 + -1.8 248,956 + -0.5 248,956 + -0.8 248,956 + -1.1 248,956 + -1.5 248,956 + -1.3 248,956 + -1.3 248,956 + -0.8 248,956 + -1.9 248,956 + -2.8 248,956 + -1.5 248,956 + -0.6 248,956 + -1.6 248,956 + -0.5 248,956 + -1.5 248,956 + -2.4 248,956 + -1.3 248,956 + -1.1 248,956 + -1.6 248,957 - -1.3 248,957 - -0.8 248,957 - -0.5 248,957 - -0.5 248,957 - -1.1 248,957 - -0.5 248,957 - -2.2 248,957 - -1.9 248,957 - -0.8 248,957 - -2.4 248,957 - -0.5 248,957 - -1.3 248,957 - -1.1 248,957 - -0.5 248,957 - -1.1 248,957 - -1.8 249,133 + cpsB OKGIIK_01155 0.22 -1.1 249,133 + cpsB OKGIIK_01155 0.22 -1.1 249,133 + cpsB OKGIIK_01155 0.22 -2.4 249,134 - cpsB OKGIIK_01155 0.22 -1.6 249,134 - cpsB OKGIIK_01155 0.22 -3.8 249,134 - cpsB OKGIIK_01155 0.22 -2.7 249,134 - cpsB OKGIIK_01155 0.22 -1.9 249,568 + cpsB OKGIIK_01155 0.53 -0.8 249,626 - cpsB OKGIIK_01155 0.57 -1.9 249,667 + cpsB OKGIIK_01155 0.60 -1.1 249,685 + cpsB OKGIIK_01155 0.61 -1.3 249,686 - cpsB OKGIIK_01155 0.61 -0.8 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,772 + cpsB OKGIIK_01155 0.67 -2.1 249,772 + cpsB OKGIIK_01155 0.67 -0.5 249,773 - cpsB OKGIIK_01155 0.67 -1.6 249,906 + cpsB OKGIIK_01155 0.77 -1.5 249,907 - cpsB OKGIIK_01155 0.77 -0.8 249,907 - cpsB OKGIIK_01155 0.77 -1.1 249,907 - cpsB OKGIIK_01155 0.77 -1.8 250,268 - -2.5 250,348 - rfbD OKGIIK_01160 0.10 +0.1 250,781 + rfbD OKGIIK_01160 0.57 +0.5 250,817 + rfbD OKGIIK_01160 0.60 -2.2 250,817 + rfbD OKGIIK_01160 0.60 -0.5 250,817 + rfbD OKGIIK_01160 0.60 +0.7 250,847 + rfbD OKGIIK_01160 0.64 +0.5 250,847 + rfbD OKGIIK_01160 0.64 -0.8 250,847 + rfbD OKGIIK_01160 0.64 -0.5 250,848 - rfbD OKGIIK_01160 0.64 -0.5 250,848 - rfbD OKGIIK_01160 0.64 -1.1 250,859 + rfbD OKGIIK_01160 0.65 -1.3 250,859 + rfbD OKGIIK_01160 0.65 -1.2 250,859 + rfbD OKGIIK_01160 0.65 -0.5 250,860 - rfbD OKGIIK_01160 0.65 -0.5 250,860 - rfbD OKGIIK_01160 0.65 -0.2 250,860 - rfbD OKGIIK_01160 0.65 -0.5 250,973 + rfbD OKGIIK_01160 0.77 -0.8 250,974 - rfbD OKGIIK_01160 0.77 -0.5 250,974 - rfbD OKGIIK_01160 0.77 -0.5 251,176 + -0.2 251,176 + +1.2 251,176 + +0.3 251,176 + +0.7 251,177 - -1.1 251,177 - +1.8 251,177 - +1.4 251,177 - -0.5 251,177 - -0.5 251,177 - -0.5 251,203 + -0.5 251,203 + +0.1 251,203 + -1.6 251,204 - -0.3
Or see this region's nucleotide sequence