Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt OKGIIK_00980 and OKGIIK_00985 are separated by 81 nucleotides OKGIIK_00985 and OKGIIK_00990 are separated by 61 nucleotides OKGIIK_00990 and OKGIIK_00995 are separated by 66 nucleotides OKGIIK_00995 and OKGIIK_01000 overlap by 17 nucleotides OKGIIK_01000 and OKGIIK_01005 overlap by 4 nucleotides
OKGIIK_00980: OKGIIK_00980 - Multidrug transporter AcrB, at 212,051 to 215,314
_00980
OKGIIK_00985: OKGIIK_00985 - DnrO protein, at 215,396 to 215,893
_00985
OKGIIK_00990: OKGIIK_00990 - TonB-dependent copper receptor, at 215,955 to 218,099
_00990
OKGIIK_00995: OKGIIK_00995 - DUF2946 domain-containing protein, at 218,166 to 218,573
_00995
OKGIIK_01000: OKGIIK_01000 - hypothetical protein, at 218,557 to 218,829
_01000
OKGIIK_01005: OKGIIK_01005 - Prephenate dehydrogenase, at 218,826 to 219,590
_01005
Position (kb) 215
216
217
218
219 Strain fitness (log2 ratio) -2
-1
0
1 at 215.176 kb on - strand at 215.237 kb on + strand at 215.237 kb on + strand at 215.237 kb on + strand at 215.237 kb on + strand at 215.237 kb on + strand at 215.238 kb on - strand at 215.238 kb on - strand at 215.238 kb on - strand at 215.238 kb on - strand at 215.238 kb on - strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.240 kb on + strand at 215.241 kb on - strand at 215.241 kb on - strand at 215.241 kb on - strand at 215.388 kb on + strand at 215.388 kb on + strand at 215.388 kb on + strand at 215.389 kb on - strand at 215.415 kb on + strand at 215.415 kb on + strand at 215.416 kb on - strand at 215.603 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.672 kb on + strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.673 kb on - strand, within OKGIIK_00985 at 215.797 kb on + strand, within OKGIIK_00985 at 215.820 kb on - strand, within OKGIIK_00985 at 215.828 kb on + strand, within OKGIIK_00985 at 215.828 kb on + strand, within OKGIIK_00985 at 215.829 kb on - strand, within OKGIIK_00985 at 215.829 kb on - strand, within OKGIIK_00985 at 215.829 kb on - strand, within OKGIIK_00985 at 215.829 kb on - strand, within OKGIIK_00985 at 216.064 kb on + strand at 217.144 kb on + strand, within OKGIIK_00990 at 217.385 kb on - strand, within OKGIIK_00990 at 217.651 kb on + strand, within OKGIIK_00990 at 218.216 kb on - strand, within OKGIIK_00995 at 218.216 kb on - strand, within OKGIIK_00995 at 218.216 kb on - strand, within OKGIIK_00995 at 218.275 kb on - strand, within OKGIIK_00995 at 218.336 kb on - strand, within OKGIIK_00995
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 215,176 - +0.5 215,237 + +0.7 215,237 + -0.7 215,237 + -0.8 215,237 + -0.3 215,237 + -0.1 215,238 - -0.1 215,238 - -0.2 215,238 - -2.3 215,238 - +0.5 215,238 - -1.6 215,240 + +0.3 215,240 + -1.1 215,240 + -0.2 215,240 + -0.4 215,240 + +1.0 215,240 + +0.0 215,240 + -1.1 215,240 + -0.6 215,240 + -0.1 215,240 + +0.1 215,240 + -1.0 215,241 - -0.1 215,241 - -1.6 215,241 - -0.5 215,388 + -0.2 215,388 + -0.8 215,388 + -0.1 215,389 - -0.5 215,415 + -0.5 215,415 + +0.3 215,416 - -2.7 215,603 + OKGIIK_00985 0.42 -0.7 215,672 + OKGIIK_00985 0.55 +0.5 215,672 + OKGIIK_00985 0.55 +0.2 215,672 + OKGIIK_00985 0.55 -1.3 215,672 + OKGIIK_00985 0.55 +1.1 215,672 + OKGIIK_00985 0.55 -0.3 215,672 + OKGIIK_00985 0.55 -0.3 215,672 + OKGIIK_00985 0.55 -0.3 215,672 + OKGIIK_00985 0.55 -0.7 215,672 + OKGIIK_00985 0.55 -0.3 215,672 + OKGIIK_00985 0.55 +1.4 215,672 + OKGIIK_00985 0.55 -2.1 215,672 + OKGIIK_00985 0.55 -0.3 215,672 + OKGIIK_00985 0.55 +1.2 215,672 + OKGIIK_00985 0.55 +0.3 215,672 + OKGIIK_00985 0.55 -1.1 215,672 + OKGIIK_00985 0.55 -0.2 215,672 + OKGIIK_00985 0.55 +0.7 215,672 + OKGIIK_00985 0.55 +0.5 215,672 + OKGIIK_00985 0.55 -0.8 215,672 + OKGIIK_00985 0.55 -0.8 215,672 + OKGIIK_00985 0.55 -0.2 215,672 + OKGIIK_00985 0.55 +1.3 215,672 + OKGIIK_00985 0.55 +0.6 215,672 + OKGIIK_00985 0.55 +0.6 215,672 + OKGIIK_00985 0.55 -0.5 215,672 + OKGIIK_00985 0.55 -2.1 215,672 + OKGIIK_00985 0.55 -0.9 215,672 + OKGIIK_00985 0.55 -0.4 215,672 + OKGIIK_00985 0.55 -0.1 215,672 + OKGIIK_00985 0.55 +0.2 215,672 + OKGIIK_00985 0.55 -0.3 215,673 - OKGIIK_00985 0.56 +0.9 215,673 - OKGIIK_00985 0.56 +1.1 215,673 - OKGIIK_00985 0.56 +0.5 215,673 - OKGIIK_00985 0.56 -0.5 215,673 - OKGIIK_00985 0.56 +0.8 215,673 - OKGIIK_00985 0.56 -0.8 215,673 - OKGIIK_00985 0.56 -0.4 215,673 - OKGIIK_00985 0.56 +0.2 215,673 - OKGIIK_00985 0.56 +0.7 215,673 - OKGIIK_00985 0.56 +1.0 215,673 - OKGIIK_00985 0.56 +1.7 215,673 - OKGIIK_00985 0.56 +0.5 215,673 - OKGIIK_00985 0.56 -0.9 215,673 - OKGIIK_00985 0.56 -0.7 215,673 - OKGIIK_00985 0.56 -0.2 215,673 - OKGIIK_00985 0.56 +0.5 215,673 - OKGIIK_00985 0.56 +0.7 215,673 - OKGIIK_00985 0.56 -0.1 215,673 - OKGIIK_00985 0.56 -0.5 215,673 - OKGIIK_00985 0.56 -0.5 215,673 - OKGIIK_00985 0.56 +0.1 215,797 + OKGIIK_00985 0.81 -0.9 215,820 - OKGIIK_00985 0.85 +0.3 215,828 + OKGIIK_00985 0.87 +1.2 215,828 + OKGIIK_00985 0.87 +0.9 215,829 - OKGIIK_00985 0.87 -0.1 215,829 - OKGIIK_00985 0.87 +0.8 215,829 - OKGIIK_00985 0.87 -0.3 215,829 - OKGIIK_00985 0.87 -0.1 216,064 + -0.8 217,144 + OKGIIK_00990 0.55 -1.7 217,385 - OKGIIK_00990 0.67 -1.6 217,651 + OKGIIK_00990 0.79 -2.1 218,216 - OKGIIK_00995 0.12 -0.5 218,216 - OKGIIK_00995 0.12 -0.3 218,216 - OKGIIK_00995 0.12 +1.5 218,275 - OKGIIK_00995 0.27 -0.2 218,336 - OKGIIK_00995 0.42 -1.9
Or see this region's nucleotide sequence