Experiment: R2A_PIPES with Nickel (II) chloride 424 uM
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt vgrG and OKGIIK_00595 are separated by 340 nucleotides OKGIIK_00595 and OKGIIK_00600 are separated by 10 nucleotides OKGIIK_00600 and OKGIIK_00605 overlap by 4 nucleotides
OKGIIK_00590: vgrG - baseplate assembly protein, at 131,070 to 131,576
vgrG
OKGIIK_00595: OKGIIK_00595 - hypothetical protein, at 131,917 to 132,240
_00595
OKGIIK_00600: OKGIIK_00600 - GPW-gp25 domain-containing protein, at 132,251 to 132,610
_00600
OKGIIK_00605: OKGIIK_00605 - Putative baseplate assembly protein, at 132,607 to 135,375
_00605
Position (kb) 132
133 Strain fitness (log2 ratio) -2
-1
0
1
2 at 131.289 kb on + strand, within vgrG at 131.717 kb on + strand at 131.719 kb on + strand at 131.720 kb on - strand at 131.720 kb on - strand at 131.903 kb on + strand at 131.903 kb on + strand at 131.904 kb on - strand at 131.904 kb on - strand at 132.528 kb on + strand, within OKGIIK_00600 at 132.552 kb on - strand, within OKGIIK_00600 at 132.552 kb on - strand, within OKGIIK_00600 at 132.727 kb on + strand at 132.727 kb on + strand at 132.727 kb on + strand at 132.728 kb on - strand at 132.728 kb on - strand at 132.938 kb on - strand, within OKGIIK_00605 at 132.995 kb on - strand, within OKGIIK_00605 at 133.015 kb on + strand, within OKGIIK_00605 at 133.015 kb on + strand, within OKGIIK_00605 at 133.016 kb on - strand, within OKGIIK_00605 at 133.016 kb on - strand, within OKGIIK_00605 at 133.120 kb on + strand, within OKGIIK_00605 at 133.120 kb on + strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.121 kb on - strand, within OKGIIK_00605 at 133.135 kb on + strand, within OKGIIK_00605 at 133.136 kb on - strand, within OKGIIK_00605 at 133.136 kb on - strand, within OKGIIK_00605 at 133.255 kb on + strand, within OKGIIK_00605 at 133.255 kb on + strand, within OKGIIK_00605 at 133.256 kb on - strand, within OKGIIK_00605 at 133.264 kb on + strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.265 kb on - strand, within OKGIIK_00605 at 133.268 kb on - strand, within OKGIIK_00605 at 133.294 kb on + strand, within OKGIIK_00605 at 133.294 kb on + strand, within OKGIIK_00605 at 133.294 kb on + strand, within OKGIIK_00605 at 133.295 kb on - strand, within OKGIIK_00605 at 133.351 kb on + strand, within OKGIIK_00605 at 133.351 kb on + strand, within OKGIIK_00605 at 133.351 kb on + strand, within OKGIIK_00605 at 133.352 kb on - strand, within OKGIIK_00605 at 133.531 kb on + strand, within OKGIIK_00605
Per-strain Table
Position Strand Gene LocusTag Fraction R2A_PIPES with Nickel (II) chloride 424 uM remove 131,289 + vgrG OKGIIK_00590 0.43 +0.3 131,717 + -1.4 131,719 + +0.6 131,720 - +0.5 131,720 - +0.6 131,903 + -1.3 131,903 + -0.3 131,904 - -0.3 131,904 - -0.9 132,528 + OKGIIK_00600 0.77 -0.3 132,552 - OKGIIK_00600 0.84 -0.3 132,552 - OKGIIK_00600 0.84 -1.4 132,727 + +0.8 132,727 + +2.0 132,727 + -0.2 132,728 - +0.0 132,728 - +2.1 132,938 - OKGIIK_00605 0.12 +1.5 132,995 - OKGIIK_00605 0.14 -0.3 133,015 + OKGIIK_00605 0.15 +1.0 133,015 + OKGIIK_00605 0.15 +1.2 133,016 - OKGIIK_00605 0.15 +1.5 133,016 - OKGIIK_00605 0.15 +0.8 133,120 + OKGIIK_00605 0.19 -0.6 133,120 + OKGIIK_00605 0.19 +0.8 133,121 - OKGIIK_00605 0.19 +0.8 133,121 - OKGIIK_00605 0.19 +0.8 133,121 - OKGIIK_00605 0.19 +0.1 133,121 - OKGIIK_00605 0.19 +1.2 133,121 - OKGIIK_00605 0.19 +1.2 133,121 - OKGIIK_00605 0.19 +0.2 133,121 - OKGIIK_00605 0.19 +0.1 133,135 + OKGIIK_00605 0.19 +0.2 133,136 - OKGIIK_00605 0.19 +0.8 133,136 - OKGIIK_00605 0.19 -0.5 133,255 + OKGIIK_00605 0.23 -1.7 133,255 + OKGIIK_00605 0.23 -0.5 133,256 - OKGIIK_00605 0.23 -1.1 133,264 + OKGIIK_00605 0.24 -1.8 133,265 - OKGIIK_00605 0.24 +0.5 133,265 - OKGIIK_00605 0.24 +0.9 133,265 - OKGIIK_00605 0.24 -0.6 133,265 - OKGIIK_00605 0.24 -0.6 133,265 - OKGIIK_00605 0.24 +0.1 133,265 - OKGIIK_00605 0.24 +0.5 133,265 - OKGIIK_00605 0.24 -1.1 133,268 - OKGIIK_00605 0.24 +0.1 133,294 + OKGIIK_00605 0.25 +0.7 133,294 + OKGIIK_00605 0.25 +0.0 133,294 + OKGIIK_00605 0.25 +1.4 133,295 - OKGIIK_00605 0.25 +1.2 133,351 + OKGIIK_00605 0.27 -0.8 133,351 + OKGIIK_00605 0.27 +0.5 133,351 + OKGIIK_00605 0.27 -1.1 133,352 - OKGIIK_00605 0.27 +1.3 133,531 + OKGIIK_00605 0.33 -1.1
Or see this region's nucleotide sequence