Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28630

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntychE and OKFHMN_28620 are separated by 26 nucleotidesOKFHMN_28620 and OKFHMN_28625 overlap by 25 nucleotidesOKFHMN_28625 and oppA are separated by 373 nucleotidesoppA and oppB are separated by 85 nucleotides OKFHMN_28615: ychE - NAAT family transporter YchE, at 5,491,258 to 5,491,905 ychE OKFHMN_28620: OKFHMN_28620 - hypothetical protein, at 5,491,932 to 5,492,087 _28620 OKFHMN_28625: OKFHMN_28625 - hypothetical protein, at 5,492,063 to 5,492,224 _28625 OKFHMN_28630: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 5,492,598 to 5,494,274 oppA OKFHMN_28635: oppB - oligopeptide ABC transporter permease OppB, at 5,494,360 to 5,495,280 oppB Position (kb) 5492 5493 5494 5495Strain fitness (log2 ratio) -2 -1 0 1 2at 5491.608 kb on + strand, within ychEat 5491.665 kb on + strand, within ychEat 5491.698 kb on + strand, within ychEat 5491.698 kb on + strand, within ychEat 5491.698 kb on + strand, within ychEat 5491.699 kb on - strand, within ychEat 5491.702 kb on + strand, within ychEat 5491.742 kb on + strand, within ychEat 5491.824 kb on + strand, within ychEat 5492.086 kb on - strandat 5492.144 kb on - strand, within OKFHMN_28625at 5492.593 kb on + strandat 5492.620 kb on - strandat 5493.005 kb on + strand, within oppAat 5493.293 kb on + strand, within oppAat 5493.813 kb on + strand, within oppAat 5494.171 kb on - strandat 5494.253 kb on + strandat 5494.257 kb on + strandat 5494.257 kb on + strandat 5494.547 kb on - strand, within oppBat 5494.744 kb on + strand, within oppBat 5494.805 kb on + strand, within oppBat 5494.947 kb on + strand, within oppBat 5495.180 kb on + strand, within oppBat 5495.214 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,491,608 + ychE OKFHMN_28615 0.54 -0.7
5,491,665 + ychE OKFHMN_28615 0.63 -1.9
5,491,698 + ychE OKFHMN_28615 0.68 -0.1
5,491,698 + ychE OKFHMN_28615 0.68 +0.1
5,491,698 + ychE OKFHMN_28615 0.68 -0.3
5,491,699 - ychE OKFHMN_28615 0.68 +0.1
5,491,702 + ychE OKFHMN_28615 0.69 +1.9
5,491,742 + ychE OKFHMN_28615 0.75 -1.3
5,491,824 + ychE OKFHMN_28615 0.87 -0.0
5,492,086 - +0.5
5,492,144 - OKFHMN_28625 0.50 -0.7
5,492,593 + -0.6
5,492,620 - -0.9
5,493,005 + oppA OKFHMN_28630 0.24 +1.1
5,493,293 + oppA OKFHMN_28630 0.41 -0.9
5,493,813 + oppA OKFHMN_28630 0.72 -0.9
5,494,171 - +0.9
5,494,253 + -2.3
5,494,257 + -0.2
5,494,257 + -1.1
5,494,547 - oppB OKFHMN_28635 0.20 +0.8
5,494,744 + oppB OKFHMN_28635 0.42 +0.0
5,494,805 + oppB OKFHMN_28635 0.48 -0.9
5,494,947 + oppB OKFHMN_28635 0.64 +0.5
5,495,180 + oppB OKFHMN_28635 0.89 +0.1
5,495,214 - -0.5

Or see this region's nucleotide sequence