Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28615

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntadhE and ychT are separated by 392 nucleotidesychT and ychE are separated by 30 nucleotidesychE and OKFHMN_28620 are separated by 26 nucleotidesOKFHMN_28620 and OKFHMN_28625 overlap by 25 nucleotidesOKFHMN_28625 and oppA are separated by 373 nucleotides OKFHMN_28605: adhE - bifunctional acetaldehyde-CoA/alcohol dehydrogenase, at 5,488,106 to 5,490,781 adhE OKFHMN_28610: ychT - Protein YchT, at 5,491,174 to 5,491,227 ychT OKFHMN_28615: ychE - NAAT family transporter YchE, at 5,491,258 to 5,491,905 ychE OKFHMN_28620: OKFHMN_28620 - hypothetical protein, at 5,491,932 to 5,492,087 _28620 OKFHMN_28625: OKFHMN_28625 - hypothetical protein, at 5,492,063 to 5,492,224 _28625 OKFHMN_28630: oppA - oligopeptide ABC transporter substrate-binding protein OppA, at 5,492,598 to 5,494,274 oppA Position (kb) 5491 5492Strain fitness (log2 ratio) -2 -1 0 1 2at 5490.311 kb on - strand, within adhEat 5490.422 kb on - strand, within adhEat 5490.422 kb on - strand, within adhEat 5490.464 kb on - strand, within adhEat 5490.543 kb on + strandat 5491.063 kb on + strandat 5491.107 kb on - strandat 5491.215 kb on + strand, within ychTat 5491.216 kb on - strand, within ychTat 5491.226 kb on - strandat 5491.478 kb on + strand, within ychEat 5491.608 kb on + strand, within ychEat 5491.665 kb on + strand, within ychEat 5491.698 kb on + strand, within ychEat 5491.698 kb on + strand, within ychEat 5491.698 kb on + strand, within ychEat 5491.699 kb on - strand, within ychEat 5491.702 kb on + strand, within ychEat 5491.742 kb on + strand, within ychEat 5491.824 kb on + strand, within ychEat 5492.086 kb on - strandat 5492.144 kb on - strand, within OKFHMN_28625at 5492.593 kb on + strandat 5492.620 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,490,311 - adhE OKFHMN_28605 0.82 -0.6
5,490,422 - adhE OKFHMN_28605 0.87 +0.0
5,490,422 - adhE OKFHMN_28605 0.87 +1.1
5,490,464 - adhE OKFHMN_28605 0.88 +0.9
5,490,543 + +0.9
5,491,063 + +0.1
5,491,107 - +0.1
5,491,215 + ychT OKFHMN_28610 0.76 +1.1
5,491,216 - ychT OKFHMN_28610 0.78 +0.1
5,491,226 - -0.2
5,491,478 + ychE OKFHMN_28615 0.34 -1.7
5,491,608 + ychE OKFHMN_28615 0.54 -0.7
5,491,665 + ychE OKFHMN_28615 0.63 -1.9
5,491,698 + ychE OKFHMN_28615 0.68 -0.1
5,491,698 + ychE OKFHMN_28615 0.68 +0.1
5,491,698 + ychE OKFHMN_28615 0.68 -0.3
5,491,699 - ychE OKFHMN_28615 0.68 +0.1
5,491,702 + ychE OKFHMN_28615 0.69 +1.9
5,491,742 + ychE OKFHMN_28615 0.75 -1.3
5,491,824 + ychE OKFHMN_28615 0.87 -0.0
5,492,086 - +0.5
5,492,144 - OKFHMN_28625 0.50 -0.7
5,492,593 + -0.6
5,492,620 - -0.9

Or see this region's nucleotide sequence