Strain Fitness in Escherichia coli ECRC100 around OKFHMN_28245

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntycgL and ycgM are separated by 71 nucleotidesycgM and ycgN are separated by 91 nucleotidesycgN and hlyE are separated by 206 nucleotides OKFHMN_28235: ycgL - Protein YcgL, at 5,416,501 to 5,416,794 ycgL OKFHMN_28240: ycgM - fumarylacetoacetate hydrolase family protein, at 5,416,866 to 5,417,525 ycgM OKFHMN_28245: ycgN - YcgN family cysteine cluster protein, at 5,417,617 to 5,418,063 ycgN OKFHMN_28250: hlyE - hemolysin HlyE, at 5,418,270 to 5,419,181 hlyE Position (kb) 5417 5418 5419Strain fitness (log2 ratio) -1 0 1 2 3at 5416.785 kb on + strandat 5416.869 kb on + strandat 5416.916 kb on + strandat 5416.921 kb on + strandat 5416.937 kb on + strand, within ycgMat 5416.941 kb on + strand, within ycgMat 5416.955 kb on + strand, within ycgMat 5417.320 kb on + strand, within ycgMat 5417.391 kb on - strand, within ycgMat 5417.582 kb on - strandat 5417.627 kb on + strandat 5418.100 kb on + strandat 5418.174 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,416,785 + +0.3
5,416,869 + -0.1
5,416,916 + +0.1
5,416,921 + -0.9
5,416,937 + ycgM OKFHMN_28240 0.11 -0.8
5,416,941 + ycgM OKFHMN_28240 0.11 -1.6
5,416,955 + ycgM OKFHMN_28240 0.13 +0.6
5,417,320 + ycgM OKFHMN_28240 0.69 +3.1
5,417,391 - ycgM OKFHMN_28240 0.80 +0.4
5,417,582 - -0.1
5,417,627 + +0.5
5,418,100 + +0.5
5,418,174 + +0.3

Or see this region's nucleotide sequence