Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27745

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntmlc and bioD are separated by 124 nucleotidesbioD and clcB overlap by 48 nucleotides OKFHMN_27740: mlc - DNA-binding transcriptional repressor Mlc, at 5,342,055 to 5,343,275 mlc OKFHMN_27745: bioD - dethiobiotin synthase, at 5,343,400 to 5,344,095 bioD OKFHMN_27750: clcB - voltage-gated ClC-type chloride channel ClcB, at 5,344,048 to 5,345,304 clcB Position (kb) 5343 5344 5345Strain fitness (log2 ratio) -1 0 1at 5342.522 kb on + strand, within mlcat 5342.628 kb on - strand, within mlcat 5343.179 kb on + strandat 5344.039 kb on + strandat 5344.120 kb on + strandat 5344.328 kb on - strand, within clcBat 5344.420 kb on + strand, within clcBat 5344.627 kb on - strand, within clcBat 5344.676 kb on - strand, within clcB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,342,522 + mlc OKFHMN_27740 0.38 +0.5
5,342,628 - mlc OKFHMN_27740 0.47 +0.3
5,343,179 + -0.0
5,344,039 + +0.4
5,344,120 + +1.5
5,344,328 - clcB OKFHMN_27750 0.22 +0.5
5,344,420 + clcB OKFHMN_27750 0.30 +0.1
5,344,627 - clcB OKFHMN_27750 0.46 +1.1
5,344,676 - clcB OKFHMN_27750 0.50 -0.0

Or see this region's nucleotide sequence