Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27735

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntynfM and ynfL are separated by 106 nucleotidesynfL and mlc are separated by 134 nucleotides OKFHMN_27730: ynfM - Inner membrane transport protein YnfM, at 5,339,667 to 5,340,920 ynfM OKFHMN_27735: ynfL - Uncharacterized HTH-type transcriptional regulator YnfL, at 5,341,027 to 5,341,920 ynfL OKFHMN_27740: mlc - DNA-binding transcriptional repressor Mlc, at 5,342,055 to 5,343,275 mlc Position (kb) 5341 5342Strain fitness (log2 ratio) -1 0 1 2at 5340.142 kb on + strand, within ynfMat 5340.594 kb on + strand, within ynfMat 5340.666 kb on - strand, within ynfMat 5340.698 kb on + strand, within ynfMat 5340.698 kb on + strand, within ynfMat 5340.908 kb on + strandat 5341.196 kb on + strand, within ynfLat 5341.251 kb on + strand, within ynfLat 5341.578 kb on - strand, within ynfLat 5342.522 kb on + strand, within mlcat 5342.628 kb on - strand, within mlc

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,340,142 + ynfM OKFHMN_27730 0.38 +1.1
5,340,594 + ynfM OKFHMN_27730 0.74 +0.0
5,340,666 - ynfM OKFHMN_27730 0.80 -0.7
5,340,698 + ynfM OKFHMN_27730 0.82 +2.1
5,340,698 + ynfM OKFHMN_27730 0.82 +0.8
5,340,908 + -1.5
5,341,196 + ynfL OKFHMN_27735 0.19 +0.1
5,341,251 + ynfL OKFHMN_27735 0.25 +0.3
5,341,578 - ynfL OKFHMN_27735 0.62 +0.2
5,342,522 + mlc OKFHMN_27740 0.38 +0.5
5,342,628 - mlc OKFHMN_27740 0.47 +0.3

Or see this region's nucleotide sequence