Strain Fitness in Escherichia coli ECRC100 around OKFHMN_27325

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_27315 and ydhU overlap by 4 nucleotidesydhU and ydhT are separated by 51 nucleotidesydhT and ydhS are separated by 11 nucleotides OKFHMN_27315: OKFHMN_27315 - Uncharacterized ferredoxin-like protein YdhX, at 5,259,696 to 5,260,364 _27315 OKFHMN_27320: ydhU - Putative cytochrome YdhU, at 5,260,361 to 5,261,146 ydhU OKFHMN_27325: ydhT - protein YdhT, at 5,261,198 to 5,261,962 ydhT OKFHMN_27330: ydhS - FAD-NAD(P)-binding protein, at 5,261,974 to 5,263,578 ydhS Position (kb) 5261 5262Strain fitness (log2 ratio) -1 0 1at 5260.409 kb on + strandat 5261.044 kb on + strand, within ydhUat 5261.501 kb on - strand, within ydhTat 5261.510 kb on - strand, within ydhTat 5261.964 kb on - strandat 5262.151 kb on - strand, within ydhSat 5262.153 kb on - strand, within ydhSat 5262.462 kb on + strand, within ydhSat 5262.797 kb on - strand, within ydhSat 5262.813 kb on + strand, within ydhS

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,260,409 + -0.3
5,261,044 + ydhU OKFHMN_27320 0.87 +1.1
5,261,501 - ydhT OKFHMN_27325 0.40 -0.7
5,261,510 - ydhT OKFHMN_27325 0.41 +1.6
5,261,964 - -0.7
5,262,151 - ydhS OKFHMN_27330 0.11 +0.1
5,262,153 - ydhS OKFHMN_27330 0.11 +1.6
5,262,462 + ydhS OKFHMN_27330 0.30 -1.7
5,262,797 - ydhS OKFHMN_27330 0.51 -0.0
5,262,813 + ydhS OKFHMN_27330 0.52 +1.7

Or see this region's nucleotide sequence