Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sufS and sufE are separated by 12 nucleotides sufE and ldtE are separated by 148 nucleotides ldtE and lpp are separated by 62 nucleotides lpp and pykF are separated by 310 nucleotides
OKFHMN_27270: sufS - cysteine desulfurase SufS, at 5,250,186 to 5,251,406
sufS
OKFHMN_27275: sufE - cysteine desulfuration protein SufE, at 5,251,419 to 5,251,835
sufE
OKFHMN_27280: ldtE - L,D-transpeptidase LdtE, at 5,251,984 to 5,252,988
ldtE
OKFHMN_27285: lpp - murein lipoprotein Lpp, at 5,253,051 to 5,253,287
lpp
OKFHMN_27290: pykF - pyruvate kinase PykF, at 5,253,598 to 5,255,010
pykF
Position (kb) 5251
5252
5253 Strain fitness (log2 ratio) -1
0
1
2 at 5251.119 kb on + strand, within sufS at 5251.280 kb on + strand, within sufS at 5251.296 kb on + strand at 5251.301 kb on + strand at 5251.314 kb on - strand at 5251.418 kb on + strand at 5251.829 kb on + strand at 5251.833 kb on + strand at 5252.107 kb on + strand, within ldtE at 5252.305 kb on + strand, within ldtE at 5252.352 kb on + strand, within ldtE at 5252.698 kb on + strand, within ldtE at 5252.831 kb on + strand, within ldtE at 5252.861 kb on - strand, within ldtE at 5252.967 kb on + strand at 5252.987 kb on - strand at 5252.992 kb on + strand at 5253.050 kb on - strand at 5253.243 kb on - strand, within lpp at 5253.302 kb on - strand at 5253.312 kb on - strand at 5253.329 kb on - strand at 5253.335 kb on - strand at 5253.493 kb on + strand at 5253.665 kb on - strand at 5253.665 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 5,251,119 + sufS OKFHMN_27270 0.76 -0.7 5,251,280 + sufS OKFHMN_27270 0.90 +0.8 5,251,296 + +1.1 5,251,301 + +0.1 5,251,314 - -0.5 5,251,418 + +0.5 5,251,829 + +2.4 5,251,833 + +0.1 5,252,107 + ldtE OKFHMN_27280 0.12 +0.5 5,252,305 + ldtE OKFHMN_27280 0.32 +2.1 5,252,352 + ldtE OKFHMN_27280 0.37 +0.8 5,252,698 + ldtE OKFHMN_27280 0.71 -0.5 5,252,831 + ldtE OKFHMN_27280 0.84 +0.1 5,252,861 - ldtE OKFHMN_27280 0.87 +0.1 5,252,967 + -0.2 5,252,987 - -0.8 5,252,992 + -0.9 5,253,050 - +0.5 5,253,243 - lpp OKFHMN_27285 0.81 +0.4 5,253,302 - -0.7 5,253,312 - +0.3 5,253,329 - +0.7 5,253,335 - +0.3 5,253,493 + +0.5 5,253,665 - +1.9 5,253,665 - +0.1
Or see this region's nucleotide sequence