Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26785

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntydjF and OKFHMN_26780 are separated by 117 nucleotidesOKFHMN_26780 and OKFHMN_26785 overlap by 26 nucleotidesOKFHMN_26785 and tnp-IS629 overlap by 1 nucleotidestnp-IS629 and ydjE are separated by 152 nucleotides OKFHMN_26775: ydjF - Uncharacterized HTH-type transcriptional regulator YdjF, at 5,153,948 to 5,154,706 ydjF OKFHMN_26780: OKFHMN_26780 - Insertion sequence 2 OrfA protein, at 5,154,824 to 5,154,994 _26780 OKFHMN_26785: OKFHMN_26785 - IS3 family transposase, at 5,154,969 to 5,155,856 _26785 OKFHMN_26790: tnp-IS629 - IS3 family IS629 transposase, at 5,155,856 to 5,156,182 tnp-IS629 OKFHMN_26795: ydjE - Inner membrane metabolite transport protein YdjE, at 5,156,335 to 5,157,495 ydjE Position (kb) 5154 5155 5156Strain fitness (log2 ratio) -1 0 1at 5154.145 kb on + strand, within ydjFat 5154.155 kb on + strand, within ydjFat 5154.226 kb on + strand, within ydjFat 5154.253 kb on + strand, within ydjFat 5154.256 kb on + strand, within ydjFat 5154.381 kb on + strand, within ydjFat 5154.616 kb on + strand, within ydjFat 5154.625 kb on + strand, within ydjFat 5154.629 kb on + strand, within ydjFat 5154.633 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,154,145 + ydjF OKFHMN_26775 0.26 +0.6
5,154,155 + ydjF OKFHMN_26775 0.27 -1.6
5,154,226 + ydjF OKFHMN_26775 0.37 -1.2
5,154,253 + ydjF OKFHMN_26775 0.40 +0.4
5,154,256 + ydjF OKFHMN_26775 0.41 +0.4
5,154,381 + ydjF OKFHMN_26775 0.57 -0.8
5,154,616 + ydjF OKFHMN_26775 0.88 -0.3
5,154,625 + ydjF OKFHMN_26775 0.89 +0.8
5,154,629 + ydjF OKFHMN_26775 0.90 -0.2
5,154,633 + -0.3

Or see this region's nucleotide sequence