Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26340

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntzwf and edd are separated by 234 nucleotidesedd and kdgA are separated by 36 nucleotideskdgA and purT are separated by 55 nucleotides OKFHMN_26335: zwf - glucose-6-phosphate dehydrogenase, at 5,072,274 to 5,073,749 zwf OKFHMN_26340: edd - phosphogluconate dehydratase, at 5,073,984 to 5,075,795 edd OKFHMN_26345: kdgA - bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase, at 5,075,832 to 5,076,473 kdgA OKFHMN_26350: purT - formate-dependent phosphoribosylglycinamide formyltransferase, at 5,076,529 to 5,077,707 purT Position (kb) 5073 5074 5075 5076Strain fitness (log2 ratio) -2 -1 0 1at 5073.073 kb on + strand, within zwfat 5073.135 kb on + strand, within zwfat 5073.273 kb on + strand, within zwfat 5073.495 kb on + strand, within zwfat 5073.583 kb on + strand, within zwfat 5073.890 kb on - strandat 5074.204 kb on - strand, within eddat 5074.234 kb on - strand, within eddat 5074.243 kb on - strand, within eddat 5074.647 kb on - strand, within eddat 5074.653 kb on + strand, within eddat 5074.799 kb on + strand, within eddat 5075.443 kb on + strand, within eddat 5075.558 kb on - strand, within eddat 5075.794 kb on - strandat 5076.155 kb on + strand, within kdgAat 5076.156 kb on - strand, within kdgAat 5076.211 kb on - strand, within kdgAat 5076.320 kb on + strand, within kdgAat 5076.336 kb on + strand, within kdgAat 5076.337 kb on - strand, within kdgAat 5076.454 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,073,073 + zwf OKFHMN_26335 0.54 -2.3
5,073,135 + zwf OKFHMN_26335 0.58 +0.7
5,073,273 + zwf OKFHMN_26335 0.68 -0.9
5,073,495 + zwf OKFHMN_26335 0.83 -1.9
5,073,583 + zwf OKFHMN_26335 0.89 -0.9
5,073,890 - -0.2
5,074,204 - edd OKFHMN_26340 0.12 +0.0
5,074,234 - edd OKFHMN_26340 0.14 +0.0
5,074,243 - edd OKFHMN_26340 0.14 +1.7
5,074,647 - edd OKFHMN_26340 0.37 -0.2
5,074,653 + edd OKFHMN_26340 0.37 +0.5
5,074,799 + edd OKFHMN_26340 0.45 +0.1
5,075,443 + edd OKFHMN_26340 0.81 +0.7
5,075,558 - edd OKFHMN_26340 0.87 +0.7
5,075,794 - +0.7
5,076,155 + kdgA OKFHMN_26345 0.50 +0.2
5,076,156 - kdgA OKFHMN_26345 0.50 -0.5
5,076,211 - kdgA OKFHMN_26345 0.59 +0.4
5,076,320 + kdgA OKFHMN_26345 0.76 -1.6
5,076,336 + kdgA OKFHMN_26345 0.79 -0.1
5,076,337 - kdgA OKFHMN_26345 0.79 -0.1
5,076,454 + -0.1

Or see this region's nucleotide sequence