Strain Fitness in Escherichia coli ECRC100 around OKFHMN_26170

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntflhB and flhA overlap by 8 nucleotidesflhA and flhe overlap by 4 nucleotidesflhe and yecT are separated by 119 nucleotides OKFHMN_26165: flhB - flagellar biosynthesis protein FlhB, at 5,042,298 to 5,043,446 flhB OKFHMN_26170: flhA - flagellar biosynthesis protein FlhA, at 5,043,439 to 5,045,517 flhA OKFHMN_26175: flhe - flagellar protein FlhE, at 5,045,514 to 5,045,909 flhe OKFHMN_26180: yecT - Uncharacterized protein YecT, at 5,046,029 to 5,046,517 yecT Position (kb) 5043 5044 5045 5046Strain fitness (log2 ratio) -1 0 1at 5043.014 kb on + strand, within flhBat 5043.386 kb on + strandat 5043.582 kb on + strandat 5044.044 kb on + strand, within flhAat 5044.164 kb on + strand, within flhAat 5044.165 kb on - strand, within flhAat 5044.508 kb on + strand, within flhAat 5044.927 kb on - strand, within flhAat 5045.321 kb on + strandat 5045.444 kb on + strandat 5045.531 kb on - strandat 5046.118 kb on + strand, within yecTat 5046.152 kb on - strand, within yecTat 5046.155 kb on + strand, within yecT

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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5,043,014 + flhB OKFHMN_26165 0.62 -1.2
5,043,386 + +1.3
5,043,582 + -0.4
5,044,044 + flhA OKFHMN_26170 0.29 -1.4
5,044,164 + flhA OKFHMN_26170 0.35 -1.0
5,044,165 - flhA OKFHMN_26170 0.35 -0.6
5,044,508 + flhA OKFHMN_26170 0.51 -1.1
5,044,927 - flhA OKFHMN_26170 0.72 -1.0
5,045,321 + -0.0
5,045,444 + -0.8
5,045,531 - +0.4
5,046,118 + yecT OKFHMN_26180 0.18 -0.5
5,046,152 - yecT OKFHMN_26180 0.25 -0.1
5,046,155 + yecT OKFHMN_26180 0.26 +0.4

Or see this region's nucleotide sequence