Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25600

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntyedJ and dcm are separated by 66 nucleotidesdcm and vsr overlap by 20 nucleotidesvsr and yedA overlap by 12 nucleotides OKFHMN_25595: yedJ - phosphohydrolase, at 4,953,504 to 4,954,199 yedJ OKFHMN_25600: dcm - DNA-cytosine methyltransferase, at 4,954,266 to 4,955,684 dcm OKFHMN_25605: vsr - VSPR family DNA mismatch endonuclease, at 4,955,665 to 4,956,135 vsr OKFHMN_25610: yedA - drug/metabolite exporter YedA, at 4,956,124 to 4,957,044 yedA Position (kb) 4954 4955 4956Strain fitness (log2 ratio) -1 0 1at 4953.277 kb on + strandat 4953.472 kb on + strandat 4953.511 kb on + strandat 4953.512 kb on - strandat 4954.045 kb on + strand, within yedJat 4954.174 kb on + strandat 4954.225 kb on + strandat 4954.348 kb on - strandat 4954.348 kb on - strandat 4954.518 kb on + strand, within dcmat 4954.745 kb on + strand, within dcmat 4955.256 kb on + strand, within dcmat 4955.257 kb on - strand, within dcmat 4955.850 kb on + strand, within vsrat 4955.850 kb on + strand, within vsrat 4955.885 kb on - strand, within vsrat 4955.933 kb on + strand, within vsrat 4955.933 kb on + strand, within vsrat 4956.021 kb on - strand, within vsrat 4956.347 kb on - strand, within yedAat 4956.351 kb on - strand, within yedAat 4956.351 kb on - strand, within yedAat 4956.354 kb on + strand, within yedAat 4956.354 kb on + strand, within yedAat 4956.355 kb on - strand, within yedAat 4956.355 kb on - strand, within yedAat 4956.355 kb on - strand, within yedAat 4956.355 kb on - strand, within yedAat 4956.356 kb on + strand, within yedAat 4956.469 kb on + strand, within yedAat 4956.517 kb on + strand, within yedA

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,953,277 + +1.0
4,953,472 + +0.3
4,953,511 + -0.2
4,953,512 - -0.6
4,954,045 + yedJ OKFHMN_25595 0.78 -0.7
4,954,174 + +1.3
4,954,225 + +0.3
4,954,348 - +1.3
4,954,348 - -1.1
4,954,518 + dcm OKFHMN_25600 0.18 +0.6
4,954,745 + dcm OKFHMN_25600 0.34 -0.7
4,955,256 + dcm OKFHMN_25600 0.70 +0.3
4,955,257 - dcm OKFHMN_25600 0.70 -1.2
4,955,850 + vsr OKFHMN_25605 0.39 -0.1
4,955,850 + vsr OKFHMN_25605 0.39 +0.3
4,955,885 - vsr OKFHMN_25605 0.47 -1.3
4,955,933 + vsr OKFHMN_25605 0.57 -0.7
4,955,933 + vsr OKFHMN_25605 0.57 +0.3
4,956,021 - vsr OKFHMN_25605 0.76 +0.7
4,956,347 - yedA OKFHMN_25610 0.24 +0.0
4,956,351 - yedA OKFHMN_25610 0.25 -0.5
4,956,351 - yedA OKFHMN_25610 0.25 -0.7
4,956,354 + yedA OKFHMN_25610 0.25 +0.3
4,956,354 + yedA OKFHMN_25610 0.25 -0.1
4,956,355 - yedA OKFHMN_25610 0.25 -1.3
4,956,355 - yedA OKFHMN_25610 0.25 -0.3
4,956,355 - yedA OKFHMN_25610 0.25 -0.1
4,956,355 - yedA OKFHMN_25610 0.25 -1.7
4,956,356 + yedA OKFHMN_25610 0.25 +1.7
4,956,469 + yedA OKFHMN_25610 0.37 -0.7
4,956,517 + yedA OKFHMN_25610 0.43 -1.6

Or see this region's nucleotide sequence