Strain Fitness in Escherichia coli ECRC100 around OKFHMN_25320

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntOKFHMN_25305 and OKFHMN_25315 are separated by 186 nucleotidesOKFHMN_25315 and OKFHMN_25320 are separated by 447 nucleotidesOKFHMN_25320 and rusA are separated by 12 nucleotidesrusA and OKFHMN_25330 overlap by 4 nucleotides OKFHMN_25305: OKFHMN_25305 - hypothetical protein, at 4,909,851 to 4,909,955 _25305 OKFHMN_25315: OKFHMN_25315 - protein hokC, at 4,910,142 to 4,910,354 _25315 OKFHMN_25320: OKFHMN_25320 - DUF968 domain-containing protein, at 4,910,802 to 4,911,851 _25320 OKFHMN_25325: rusA - Endodeoxyribonuclease RusA, at 4,911,864 to 4,912,223 rusA OKFHMN_25330: OKFHMN_25330 - antiterminator, at 4,912,220 to 4,912,909 _25330 Position (kb) 4910 4911 4912Strain fitness (log2 ratio) -1 0 1 2at 4909.850 kb on + strandat 4909.914 kb on + strand, within OKFHMN_25305at 4910.466 kb on - strandat 4910.900 kb on - strandat 4911.071 kb on + strand, within OKFHMN_25320at 4911.072 kb on - strand, within OKFHMN_25320at 4911.283 kb on + strand, within OKFHMN_25320at 4911.415 kb on + strand, within OKFHMN_25320at 4911.416 kb on - strand, within OKFHMN_25320at 4911.442 kb on + strand, within OKFHMN_25320at 4911.573 kb on - strand, within OKFHMN_25320at 4912.232 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,909,850 + +0.7
4,909,914 + OKFHMN_25305 0.60 -0.5
4,910,466 - +0.3
4,910,900 - +1.4
4,911,071 + OKFHMN_25320 0.26 -1.3
4,911,072 - OKFHMN_25320 0.26 +2.2
4,911,283 + OKFHMN_25320 0.46 -1.1
4,911,415 + OKFHMN_25320 0.58 +0.2
4,911,416 - OKFHMN_25320 0.58 -0.4
4,911,442 + OKFHMN_25320 0.61 +0.3
4,911,573 - OKFHMN_25320 0.73 -1.1
4,912,232 + -0.4

Or see this region's nucleotide sequence