Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24415

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntwcaA and wzy overlap by 4 nucleotideswzy and wcaA overlap by 32 nucleotideswcaA and wzx overlap by 14 nucleotides OKFHMN_24410: wcaA - glycosyl transferase, at 4,768,520 to 4,769,302 wcaA OKFHMN_24415: wzy - O157 family O-antigen polymerase, at 4,769,299 to 4,770,483 wzy OKFHMN_24420: wcaA - glycosyl transferase, at 4,770,452 to 4,771,198 wcaA OKFHMN_24425: wzx - O157 family O-antigen flippase, at 4,771,185 to 4,772,576 wzx Position (kb) 4769 4770 4771Strain fitness (log2 ratio) -2 -1 0 1at 4768.307 kb on + strandat 4768.336 kb on + strandat 4770.247 kb on + strand, within wzyat 4770.429 kb on + strandat 4771.184 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,768,307 + -0.7
4,768,336 + -0.5
4,770,247 + wzy OKFHMN_24415 0.80 -2.2
4,770,429 + +0.3
4,771,184 + -0.4

Or see this region's nucleotide sequence