Strain Fitness in Escherichia coli ECRC100 around OKFHMN_24240

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntibsA and terY are separated by 220 nucleotidesterY and pphC are separated by 77 nucleotidespphC and yegI overlap by 4 nucleotides OKFHMN_24230: ibsA - Small toxic protein IbsA, at 4,727,281 to 4,727,340 ibsA OKFHMN_24235: terY - Uncharacterized protein YegL, at 4,727,561 to 4,728,220 terY OKFHMN_24240: pphC - Serine/threonine-protein phosphatase 3, at 4,728,298 to 4,728,978 pphC OKFHMN_24245: yegI - protein kinase YegI, at 4,728,975 to 4,730,915 yegI Position (kb) 4728 4729Strain fitness (log2 ratio) -2 -1 0 1 2 3at 4727.310 kb on - strand, within ibsAat 4727.310 kb on - strand, within ibsAat 4727.339 kb on - strandat 4727.407 kb on - strandat 4727.880 kb on + strand, within terYat 4727.914 kb on + strand, within terYat 4728.058 kb on + strand, within terYat 4728.333 kb on - strandat 4728.418 kb on + strand, within pphCat 4728.418 kb on + strand, within pphCat 4728.522 kb on + strand, within pphCat 4728.673 kb on + strand, within pphCat 4728.769 kb on - strand, within pphCat 4728.933 kb on + strandat 4728.988 kb on + strandat 4728.988 kb on + strandat 4728.989 kb on - strandat 4728.989 kb on - strandat 4728.989 kb on - strandat 4728.990 kb on + strandat 4728.991 kb on - strandat 4728.992 kb on + strandat 4728.992 kb on + strandat 4729.118 kb on - strandat 4729.303 kb on - strand, within yegIat 4729.332 kb on + strand, within yegIat 4729.332 kb on + strand, within yegIat 4729.420 kb on + strand, within yegIat 4729.652 kb on + strand, within yegIat 4729.707 kb on + strand, within yegI

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,727,310 - ibsA OKFHMN_24230 0.48 +0.5
4,727,310 - ibsA OKFHMN_24230 0.48 -0.0
4,727,339 - -0.4
4,727,407 - +0.2
4,727,880 + terY OKFHMN_24235 0.48 -0.5
4,727,914 + terY OKFHMN_24235 0.53 +0.2
4,728,058 + terY OKFHMN_24235 0.75 +0.8
4,728,333 - +0.2
4,728,418 + pphC OKFHMN_24240 0.18 -0.9
4,728,418 + pphC OKFHMN_24240 0.18 -0.3
4,728,522 + pphC OKFHMN_24240 0.33 -1.3
4,728,673 + pphC OKFHMN_24240 0.55 +1.2
4,728,769 - pphC OKFHMN_24240 0.69 -0.4
4,728,933 + -0.4
4,728,988 + +0.1
4,728,988 + +0.8
4,728,989 - +0.8
4,728,989 - +3.2
4,728,989 - -0.3
4,728,990 + +0.1
4,728,991 - -0.6
4,728,992 + -0.6
4,728,992 + -0.9
4,729,118 - +1.5
4,729,303 - yegI OKFHMN_24245 0.17 -0.0
4,729,332 + yegI OKFHMN_24245 0.18 -2.6
4,729,332 + yegI OKFHMN_24245 0.18 +0.6
4,729,420 + yegI OKFHMN_24245 0.23 +0.3
4,729,652 + yegI OKFHMN_24245 0.35 -1.1
4,729,707 + yegI OKFHMN_24245 0.38 -1.9

Or see this region's nucleotide sequence