Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt menD and menH overlap by 4 nucleotides menH and menB are separated by 14 nucleotides menB and menC overlap by 1 nucleotides menC and menE overlap by 4 nucleotides
OKFHMN_22935: menD - 2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene- 1-carboxylic-acid synthase, at 4,464,897 to 4,466,567
menD
OKFHMN_22940: menH - 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxy late synthase, at 4,466,564 to 4,467,322
menH
OKFHMN_22945: menB - 1,4-dihydroxy-2-naphthoyl-CoA synthase, at 4,467,337 to 4,468,194
menB
OKFHMN_22950: menC - o-succinylbenzoate synthase, at 4,468,194 to 4,469,156
menC
OKFHMN_22955: menE - o-succinylbenzoate--CoA ligase, at 4,469,153 to 4,470,508
menE
Position (kb) 4467
4468
4469 Strain fitness (log2 ratio) -2
-1
0
1
2 at 4466.554 kb on + strand at 4466.564 kb on - strand at 4466.604 kb on + strand at 4466.693 kb on + strand, within menH at 4466.693 kb on + strand, within menH at 4466.763 kb on + strand, within menH at 4466.764 kb on - strand, within menH at 4466.786 kb on + strand, within menH at 4467.148 kb on + strand, within menH at 4467.364 kb on + strand at 4467.529 kb on + strand, within menB at 4467.927 kb on + strand, within menB at 4468.394 kb on - strand, within menC at 4468.453 kb on + strand, within menC at 4468.454 kb on - strand, within menC at 4468.562 kb on + strand, within menC at 4468.562 kb on + strand, within menC at 4468.603 kb on - strand, within menC at 4468.603 kb on - strand, within menC at 4468.888 kb on + strand, within menC at 4468.999 kb on + strand, within menC at 4469.000 kb on - strand, within menC at 4469.000 kb on - strand, within menC at 4469.074 kb on + strand at 4469.092 kb on + strand at 4469.152 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 4,466,554 + +0.2 4,466,564 - -1.0 4,466,604 + -0.5 4,466,693 + menH OKFHMN_22940 0.17 +1.9 4,466,693 + menH OKFHMN_22940 0.17 +1.4 4,466,763 + menH OKFHMN_22940 0.26 -0.4 4,466,764 - menH OKFHMN_22940 0.26 -0.4 4,466,786 + menH OKFHMN_22940 0.29 +0.0 4,467,148 + menH OKFHMN_22940 0.77 -0.6 4,467,364 + +0.3 4,467,529 + menB OKFHMN_22945 0.22 -1.2 4,467,927 + menB OKFHMN_22945 0.69 -2.0 4,468,394 - menC OKFHMN_22950 0.21 +1.4 4,468,453 + menC OKFHMN_22950 0.27 -1.0 4,468,454 - menC OKFHMN_22950 0.27 +0.4 4,468,562 + menC OKFHMN_22950 0.38 +0.7 4,468,562 + menC OKFHMN_22950 0.38 -0.4 4,468,603 - menC OKFHMN_22950 0.42 +0.4 4,468,603 - menC OKFHMN_22950 0.42 -0.3 4,468,888 + menC OKFHMN_22950 0.72 +0.6 4,468,999 + menC OKFHMN_22950 0.84 -0.2 4,469,000 - menC OKFHMN_22950 0.84 +0.4 4,469,000 - menC OKFHMN_22950 0.84 -0.6 4,469,074 + +0.1 4,469,092 + +0.1 4,469,152 + -0.2
Or see this region's nucleotide sequence