Strain Fitness in Escherichia coli ECRC100 around OKFHMN_22625

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntaroC and mepA are separated by 3 nucleotidesmepA and yfcA overlap by 1 nucleotidesyfcA and epmC overlap by 1 nucleotidesepmC and yfcL are separated by 33 nucleotidesyfcL and mnmC are separated by 120 nucleotides OKFHMN_22615: aroC - chorismate synthase, at 4,400,743 to 4,401,828 aroC OKFHMN_22620: mepA - penicillin-insensitive murein endopeptidase, at 4,401,832 to 4,402,656 mepA OKFHMN_22625: yfcA - putative membrane transporter protein YfcA, at 4,402,656 to 4,403,465 yfcA OKFHMN_22630: epmC - elongation factor P hydroxylase, at 4,403,465 to 4,404,013 epmC OKFHMN_22635: yfcL - Uncharacterized protein YfcL, at 4,404,047 to 4,404,325 yfcL OKFHMN_22640: mnmC - bifunctional tRNA (5-methylaminomethyl-2-thiouridine)(34)-methyltransferase MnmD/FAD-dependent 5-carboxymethylaminomethyl-2-thiouridine(34) oxidoreductase MnmC, at 4,404,446 to 4,406,452 mnmC Position (kb) 4402 4403 4404Strain fitness (log2 ratio) -1 0 1 2at 4401.928 kb on - strand, within mepAat 4401.963 kb on + strand, within mepAat 4402.077 kb on + strand, within mepAat 4402.259 kb on + strand, within mepAat 4402.365 kb on + strand, within mepAat 4402.467 kb on - strand, within mepAat 4402.467 kb on - strand, within mepAat 4402.679 kb on + strandat 4402.862 kb on + strand, within yfcAat 4402.910 kb on + strand, within yfcAat 4402.911 kb on - strand, within yfcAat 4402.913 kb on + strand, within yfcAat 4402.957 kb on + strand, within yfcAat 4402.957 kb on + strand, within yfcAat 4402.957 kb on + strand, within yfcAat 4402.958 kb on - strand, within yfcAat 4402.963 kb on - strand, within yfcAat 4402.963 kb on - strand, within yfcAat 4402.982 kb on - strand, within yfcAat 4403.013 kb on - strand, within yfcAat 4403.023 kb on - strand, within yfcAat 4403.074 kb on - strand, within yfcAat 4403.218 kb on + strand, within yfcAat 4403.418 kb on + strandat 4403.418 kb on + strandat 4403.463 kb on + strandat 4403.464 kb on - strandat 4403.464 kb on - strandat 4403.473 kb on + strandat 4403.545 kb on + strand, within epmCat 4403.567 kb on + strand, within epmCat 4403.589 kb on + strand, within epmCat 4403.590 kb on - strand, within epmCat 4403.595 kb on - strand, within epmCat 4403.595 kb on - strand, within epmCat 4403.660 kb on - strand, within epmCat 4403.813 kb on - strand, within epmCat 4403.954 kb on + strand, within epmCat 4403.957 kb on + strand, within epmCat 4404.151 kb on + strand, within yfcLat 4404.151 kb on + strand, within yfcLat 4404.151 kb on + strand, within yfcLat 4404.152 kb on - strand, within yfcLat 4404.152 kb on - strand, within yfcLat 4404.287 kb on - strand, within yfcLat 4404.287 kb on - strand, within yfcLat 4404.324 kb on - strandat 4404.395 kb on + strandat 4404.396 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,401,928 - mepA OKFHMN_22620 0.12 +0.8
4,401,963 + mepA OKFHMN_22620 0.16 +0.2
4,402,077 + mepA OKFHMN_22620 0.30 -0.1
4,402,259 + mepA OKFHMN_22620 0.52 -0.6
4,402,365 + mepA OKFHMN_22620 0.65 -0.6
4,402,467 - mepA OKFHMN_22620 0.77 +0.4
4,402,467 - mepA OKFHMN_22620 0.77 +0.4
4,402,679 + -0.1
4,402,862 + yfcA OKFHMN_22625 0.25 +0.9
4,402,910 + yfcA OKFHMN_22625 0.31 +0.5
4,402,911 - yfcA OKFHMN_22625 0.31 -0.0
4,402,913 + yfcA OKFHMN_22625 0.32 -0.2
4,402,957 + yfcA OKFHMN_22625 0.37 +0.4
4,402,957 + yfcA OKFHMN_22625 0.37 +0.0
4,402,957 + yfcA OKFHMN_22625 0.37 -0.4
4,402,958 - yfcA OKFHMN_22625 0.37 +0.1
4,402,963 - yfcA OKFHMN_22625 0.38 +0.6
4,402,963 - yfcA OKFHMN_22625 0.38 +0.0
4,402,982 - yfcA OKFHMN_22625 0.40 +1.4
4,403,013 - yfcA OKFHMN_22625 0.44 +1.2
4,403,023 - yfcA OKFHMN_22625 0.45 +0.8
4,403,074 - yfcA OKFHMN_22625 0.52 +0.4
4,403,218 + yfcA OKFHMN_22625 0.69 -0.1
4,403,418 + +0.1
4,403,418 + +0.4
4,403,463 + -0.4
4,403,464 - +1.8
4,403,464 - -1.4
4,403,473 + -0.4
4,403,545 + epmC OKFHMN_22630 0.15 +0.9
4,403,567 + epmC OKFHMN_22630 0.19 -0.6
4,403,589 + epmC OKFHMN_22630 0.23 -0.1
4,403,590 - epmC OKFHMN_22630 0.23 +1.4
4,403,595 - epmC OKFHMN_22630 0.24 -0.2
4,403,595 - epmC OKFHMN_22630 0.24 -0.0
4,403,660 - epmC OKFHMN_22630 0.36 -0.0
4,403,813 - epmC OKFHMN_22630 0.63 +1.4
4,403,954 + epmC OKFHMN_22630 0.89 -0.4
4,403,957 + epmC OKFHMN_22630 0.90 +0.9
4,404,151 + yfcL OKFHMN_22635 0.37 +0.4
4,404,151 + yfcL OKFHMN_22635 0.37 -1.0
4,404,151 + yfcL OKFHMN_22635 0.37 -0.1
4,404,152 - yfcL OKFHMN_22635 0.38 -1.4
4,404,152 - yfcL OKFHMN_22635 0.38 +0.0
4,404,287 - yfcL OKFHMN_22635 0.86 -0.6
4,404,287 - yfcL OKFHMN_22635 0.86 +0.4
4,404,324 - +2.4
4,404,395 + +0.4
4,404,396 - -0.6

Or see this region's nucleotide sequence