Strain Fitness in Escherichia coli ECRC100 around OKFHMN_20945

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntIle2_trna and OKFHMN_20945 are separated by 751 nucleotidesOKFHMN_20945 and OKFHMN_20950 are separated by 935 nucleotides OKFHMN_20940: Ile2_trna - tRNA-Ile2(cat), at 4,068,576 to 4,068,651 _trna OKFHMN_20945: OKFHMN_20945 - DUF5507 domain-containing protein, at 4,069,403 to 4,070,752 _20945 OKFHMN_20950: OKFHMN_20950 - Uncharacterized conserved protein, contains a C-terminal beta-barrel porin domain, at 4,071,688 to 4,075,629 _20950 Position (kb) 4069 4070 4071Strain fitness (log2 ratio) -1 0 1 2at 4068.551 kb on - strandat 4068.552 kb on + strandat 4068.553 kb on - strandat 4068.569 kb on + strandat 4068.710 kb on - strandat 4068.890 kb on - strandat 4068.893 kb on + strandat 4068.913 kb on + strandat 4069.014 kb on - strandat 4069.017 kb on + strandat 4069.379 kb on + strandat 4069.460 kb on + strandat 4069.512 kb on + strandat 4069.743 kb on + strand, within OKFHMN_20945at 4070.063 kb on - strand, within OKFHMN_20945at 4070.212 kb on + strand, within OKFHMN_20945at 4070.805 kb on + strandat 4070.866 kb on + strandat 4071.044 kb on - strandat 4071.182 kb on - strandat 4071.206 kb on - strandat 4071.206 kb on - strandat 4071.251 kb on - strandat 4071.278 kb on - strandat 4071.279 kb on + strandat 4071.525 kb on + strandat 4071.569 kb on + strandat 4071.663 kb on - strandat 4071.709 kb on + strandat 4071.710 kb on - strandat 4071.740 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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4,068,551 - -0.3
4,068,552 + +0.3
4,068,553 - +0.3
4,068,569 + +0.8
4,068,710 - +1.5
4,068,890 - -0.1
4,068,893 + +0.4
4,068,913 + -1.5
4,069,014 - +0.1
4,069,017 + +0.5
4,069,379 + +0.3
4,069,460 + +0.4
4,069,512 + -0.1
4,069,743 + OKFHMN_20945 0.25 -0.6
4,070,063 - OKFHMN_20945 0.49 +0.5
4,070,212 + OKFHMN_20945 0.60 +0.5
4,070,805 + -0.4
4,070,866 + -0.1
4,071,044 - +0.8
4,071,182 - -0.3
4,071,206 - +1.5
4,071,206 - -0.1
4,071,251 - +0.1
4,071,278 - -1.2
4,071,279 + +2.5
4,071,525 + -1.7
4,071,569 + -0.3
4,071,663 - +0.5
4,071,709 + +0.1
4,071,710 - +0.5
4,071,740 + +0.2

Or see this region's nucleotide sequence