Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt sctI and escJ overlap by 4 nucleotides escJ and OKFHMN_19835 are separated by 15 nucleotides OKFHMN_19835 and ygeN overlap by 59 nucleotides ygeN and ygeN are separated by 15 nucleotides ygeN and ygeK are separated by 222 nucleotides
OKFHMN_19825: sctI - type III secretion system inner rod subunit SctI, at 3,856,124 to 3,856,456
sctI
OKFHMN_19830: escJ - EscJ/YscJ/HrcJ family type III secretion inner membrane ring protein, at 3,856,453 to 3,857,187
escJ
OKFHMN_19835: OKFHMN_19835 - type III secretion apparatus protein OrgA/MxiK, at 3,857,203 to 3,857,784
_19835
OKFHMN_19840: ygeN - Putative uncharacterized protein YgeN, at 3,857,726 to 3,857,986
ygeN
OKFHMN_19845: ygeN - Putative uncharacterized protein YgeN, at 3,858,002 to 3,858,433
ygeN
OKFHMN_19850: ygeK - Uncharacterized response regulatory protein YgeK, at 3,858,656 to 3,859,288
ygeK
Position (kb) 3857
3858 Strain fitness (log2 ratio) -1
0
1 at 3856.460 kb on - strand at 3856.963 kb on - strand, within escJ at 3857.608 kb on - strand, within OKFHMN_19835 at 3857.719 kb on - strand, within OKFHMN_19835 at 3857.825 kb on - strand, within ygeN at 3858.025 kb on - strand at 3858.142 kb on + strand, within ygeN at 3858.394 kb on + strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 3,856,460 - +0.2 3,856,963 - escJ OKFHMN_19830 0.69 -0.3 3,857,608 - OKFHMN_19835 0.70 -1.3 3,857,719 - OKFHMN_19835 0.89 +0.0 3,857,825 - ygeN OKFHMN_19840 0.38 -1.0 3,858,025 - -1.2 3,858,142 + ygeN OKFHMN_19845 0.32 +0.0 3,858,394 + -0.1
Or see this region's nucleotide sequence