Strain Fitness in Escherichia coli ECRC100 around OKFHMN_19585

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntrecJ and prfB are separated by 307 nucleotidesprfB and lysS are separated by 9 nucleotides OKFHMN_19580: recJ - single-stranded-DNA-specific exonuclease RecJ, at 3,800,136 to 3,801,869 recJ OKFHMN_19585: prfB - peptide chain release factor 2, at 3,802,177 to 3,803,058 prfB OKFHMN_19590: lysS - lysine--tRNA ligase, at 3,803,068 to 3,804,585 lysS Position (kb) 3802 3803 3804Strain fitness (log2 ratio) -2 -1 0 1at 3801.216 kb on + strand, within recJat 3801.216 kb on + strand, within recJat 3801.317 kb on + strand, within recJat 3801.357 kb on - strand, within recJat 3801.357 kb on - strand, within recJat 3801.379 kb on + strand, within recJat 3801.391 kb on - strand, within recJat 3801.741 kb on - strandat 3801.741 kb on - strandat 3801.795 kb on + strandat 3801.795 kb on + strandat 3801.944 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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3,801,216 + recJ OKFHMN_19580 0.62 -0.3
3,801,216 + recJ OKFHMN_19580 0.62 -0.3
3,801,317 + recJ OKFHMN_19580 0.68 +0.8
3,801,357 - recJ OKFHMN_19580 0.70 -0.5
3,801,357 - recJ OKFHMN_19580 0.70 +0.0
3,801,379 + recJ OKFHMN_19580 0.72 +0.7
3,801,391 - recJ OKFHMN_19580 0.72 +0.6
3,801,741 - -1.6
3,801,741 - +0.0
3,801,795 + -0.2
3,801,795 + -1.8
3,801,944 + +0.8

Or see this region's nucleotide sequence