Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15310

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescU and etgA overlap by 4 nucleotidesetgA and grlR are separated by 195 nucleotidesgrlR and grlA are separated by 54 nucleotides OKFHMN_15305: escU - type III secretion system LEE export apparatus switch protein EscU, at 2,958,785 to 2,959,822 escU OKFHMN_15310: etgA - type III secretion system LEE muramidase EtgA, at 2,959,819 to 2,960,277 etgA OKFHMN_15315: grlR - type III secretion system LEE GrlA-binding negative regulator GrlR, at 2,960,473 to 2,960,844 grlR OKFHMN_15320: grlA - type III secretion system LEE transcriptional regulator GrlA, at 2,960,899 to 2,961,312 grlA Position (kb) 2959 2960 2961Strain fitness (log2 ratio) -1 0 1at 2959.596 kb on - strand, within escUat 2959.599 kb on - strand, within escUat 2959.744 kb on + strandat 2959.806 kb on + strandat 2959.857 kb on - strandat 2960.193 kb on + strand, within etgAat 2960.232 kb on - strandat 2960.422 kb on - strandat 2960.539 kb on - strand, within grlRat 2960.624 kb on + strand, within grlRat 2960.690 kb on + strand, within grlRat 2960.806 kb on - strand, within grlRat 2960.974 kb on - strand, within grlAat 2961.253 kb on - strand, within grlAat 2961.271 kb on - strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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2,959,596 - escU OKFHMN_15305 0.78 -0.3
2,959,599 - escU OKFHMN_15305 0.78 -0.1
2,959,744 + +1.4
2,959,806 + -0.3
2,959,857 - +0.1
2,960,193 + etgA OKFHMN_15310 0.81 -0.5
2,960,232 - +1.6
2,960,422 - +0.3
2,960,539 - grlR OKFHMN_15315 0.18 -0.5
2,960,624 + grlR OKFHMN_15315 0.41 +1.3
2,960,690 + grlR OKFHMN_15315 0.58 -0.2
2,960,806 - grlR OKFHMN_15315 0.90 -0.4
2,960,974 - grlA OKFHMN_15320 0.18 -1.1
2,961,253 - grlA OKFHMN_15320 0.86 -0.2
2,961,271 - -0.1

Or see this region's nucleotide sequence