Strain Fitness in Escherichia coli ECRC100 around OKFHMN_15300

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntescL and escR are separated by 4 nucleotidesescR and escS overlap by 1 nucleotidesescS and escT overlap by 1 nucleotidesescT and escU overlap by 8 nucleotides OKFHMN_15285: escL - negative regulator EscL, stator protein, at 2,956,436 to 2,957,089 escL OKFHMN_15290: escR - Type III secretion systemminor export apparatus protein EscR, at 2,957,094 to 2,957,747 escR OKFHMN_15295: escS - type III secretion system LEE export apparatus protein EscS, at 2,957,747 to 2,958,016 escS OKFHMN_15300: escT - type III secretion system LEE export apparatus protein EscT, at 2,958,016 to 2,958,792 escT OKFHMN_15305: escU - type III secretion system LEE export apparatus switch protein EscU, at 2,958,785 to 2,959,822 escU Position (kb) 2958 2959Strain fitness (log2 ratio) -1 0 1at 2957.846 kb on - strand, within escSat 2957.846 kb on - strand, within escSat 2957.985 kb on + strand, within escSat 2958.026 kb on + strandat 2958.293 kb on + strand, within escTat 2958.339 kb on - strand, within escTat 2958.363 kb on - strand, within escTat 2958.449 kb on + strand, within escTat 2958.660 kb on - strand, within escTat 2959.596 kb on - strand, within escUat 2959.599 kb on - strand, within escUat 2959.744 kb on + strand

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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2,957,846 - escS OKFHMN_15295 0.37 -0.7
2,957,846 - escS OKFHMN_15295 0.37 -0.2
2,957,985 + escS OKFHMN_15295 0.88 -0.2
2,958,026 + -0.2
2,958,293 + escT OKFHMN_15300 0.36 -1.1
2,958,339 - escT OKFHMN_15300 0.42 +0.4
2,958,363 - escT OKFHMN_15300 0.45 -0.1
2,958,449 + escT OKFHMN_15300 0.56 -0.5
2,958,660 - escT OKFHMN_15300 0.83 -0.2
2,959,596 - escU OKFHMN_15305 0.78 -0.3
2,959,599 - escU OKFHMN_15305 0.78 -0.1
2,959,744 + +1.4

Or see this region's nucleotide sequence