Experiment: Bas58
Only strains with sufficient reads to estimate fitness are shown,
but the strain fitness values are still rather noisy and may be biased towards zero.
Strains near the edge of a gene are not shown as being associated with that
gene (they are in grey).
Strains in the central 10-90% of a gene are color coded by the insertion's strand.
Usually, "+" means that the selectable marker is encoded on the forward strand,
i.e., transcribed rightward.
500 nt tnaA and tnaC are separated by 220 nucleotides tnaC and OKFHMN_14975 are separated by 399 nucleotides OKFHMN_14975 and OKFHMN_14980 overlap by 50 nucleotides OKFHMN_14980 and OKFHMN_14985 are separated by 149 nucleotides
OKFHMN_14965: tnaA - tryptophanase, at 2,896,225 to 2,897,640
tnaA
OKFHMN_14970: tnaC - tryptophanase leader peptide, at 2,897,861 to 2,897,935
tnaC
OKFHMN_14975: OKFHMN_14975 - toxin, at 2,898,335 to 2,900,323
_14975
OKFHMN_14980: OKFHMN_14980 - hypothetical protein, at 2,900,274 to 2,900,504
_14980
OKFHMN_14985: OKFHMN_14985 - tRNA modification GTPase MnmE, at 2,900,654 to 2,902,018
_14985
Position (kb)
2898
2899
2900
2901 Strain fitness (log2 ratio)
-3
-2
-1
0
1
2 at 2897.357 kb on + strand, within tnaA at 2897.364 kb on - strand, within tnaA at 2897.400 kb on - strand, within tnaA at 2897.400 kb on - strand, within tnaA at 2897.400 kb on - strand, within tnaA at 2897.400 kb on - strand, within tnaA at 2897.420 kb on + strand, within tnaA at 2897.454 kb on + strand, within tnaA at 2897.454 kb on + strand, within tnaA at 2897.565 kb on - strand at 2897.577 kb on + strand at 2897.717 kb on + strand at 2897.807 kb on - strand at 2897.918 kb on + strand, within tnaC at 2897.976 kb on - strand at 2898.142 kb on + strand at 2898.142 kb on + strand at 2898.287 kb on - strand at 2898.298 kb on + strand at 2898.299 kb on - strand at 2898.368 kb on - strand at 2898.439 kb on - strand at 2898.633 kb on + strand, within OKFHMN_14975 at 2898.694 kb on + strand, within OKFHMN_14975 at 2898.885 kb on - strand, within OKFHMN_14975 at 2898.906 kb on + strand, within OKFHMN_14975 at 2899.047 kb on + strand, within OKFHMN_14975 at 2899.054 kb on + strand, within OKFHMN_14975 at 2899.087 kb on + strand, within OKFHMN_14975 at 2899.088 kb on - strand, within OKFHMN_14975 at 2899.088 kb on - strand, within OKFHMN_14975 at 2899.136 kb on + strand, within OKFHMN_14975 at 2899.137 kb on - strand, within OKFHMN_14975 at 2899.137 kb on - strand, within OKFHMN_14975 at 2899.137 kb on - strand, within OKFHMN_14975 at 2899.186 kb on + strand, within OKFHMN_14975 at 2899.518 kb on + strand, within OKFHMN_14975 at 2899.592 kb on - strand, within OKFHMN_14975 at 2899.636 kb on + strand, within OKFHMN_14975 at 2899.965 kb on + strand, within OKFHMN_14975 at 2900.042 kb on + strand, within OKFHMN_14975 at 2900.154 kb on + strand at 2900.280 kb on + strand at 2900.281 kb on - strand at 2900.385 kb on + strand, within OKFHMN_14980 at 2900.389 kb on + strand, within OKFHMN_14980 at 2900.636 kb on + strand at 2900.637 kb on - strand at 2900.637 kb on - strand at 2900.637 kb on - strand at 2900.637 kb on - strand at 2900.639 kb on - strand at 2900.655 kb on + strand at 2900.656 kb on - strand
Per-strain Table
Position Strand Gene LocusTag Fraction Bas58 remove 2,897,357 + tnaA OKFHMN_14965 0.80 +0.3 2,897,364 - tnaA OKFHMN_14965 0.80 +0.6 2,897,400 - tnaA OKFHMN_14965 0.83 -0.4 2,897,400 - tnaA OKFHMN_14965 0.83 +0.9 2,897,400 - tnaA OKFHMN_14965 0.83 +1.5 2,897,400 - tnaA OKFHMN_14965 0.83 -1.0 2,897,420 + tnaA OKFHMN_14965 0.84 -2.8 2,897,454 + tnaA OKFHMN_14965 0.87 +0.0 2,897,454 + tnaA OKFHMN_14965 0.87 -1.3 2,897,565 - -0.9 2,897,577 + +0.6 2,897,717 + -0.3 2,897,807 - +1.3 2,897,918 + tnaC OKFHMN_14970 0.76 -0.7 2,897,976 - +1.3 2,898,142 + -1.5 2,898,142 + +0.1 2,898,287 - +1.9 2,898,298 + +0.7 2,898,299 - +0.2 2,898,368 - +0.1 2,898,439 - -0.3 2,898,633 + OKFHMN_14975 0.15 +0.8 2,898,694 + OKFHMN_14975 0.18 -1.1 2,898,885 - OKFHMN_14975 0.28 -1.1 2,898,906 + OKFHMN_14975 0.29 +0.3 2,899,047 + OKFHMN_14975 0.36 +0.8 2,899,054 + OKFHMN_14975 0.36 +0.3 2,899,087 + OKFHMN_14975 0.38 +1.0 2,899,088 - OKFHMN_14975 0.38 +0.4 2,899,088 - OKFHMN_14975 0.38 -0.3 2,899,136 + OKFHMN_14975 0.40 +1.0 2,899,137 - OKFHMN_14975 0.40 +0.9 2,899,137 - OKFHMN_14975 0.40 -0.4 2,899,137 - OKFHMN_14975 0.40 -1.3 2,899,186 + OKFHMN_14975 0.43 -0.1 2,899,518 + OKFHMN_14975 0.59 -0.5 2,899,592 - OKFHMN_14975 0.63 +0.5 2,899,636 + OKFHMN_14975 0.65 -0.0 2,899,965 + OKFHMN_14975 0.82 -0.3 2,900,042 + OKFHMN_14975 0.86 +1.1 2,900,154 + -0.4 2,900,280 + -0.1 2,900,281 - +0.6 2,900,385 + OKFHMN_14980 0.48 +0.4 2,900,389 + OKFHMN_14980 0.50 +0.3 2,900,636 + -0.1 2,900,637 - +0.7 2,900,637 - -0.2 2,900,637 - +0.8 2,900,637 - -0.9 2,900,639 - +0.5 2,900,655 + -0.8 2,900,656 - -0.4
Or see this region's nucleotide sequence