Strain Fitness in Escherichia coli ECRC100 around OKFHMN_09970

Experiment: Bas58

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Only strains with sufficient reads to estimate fitness are shown, but the strain fitness values are still rather noisy and may be biased towards zero. Strains near the edge of a gene are not shown as being associated with that gene (they are in grey). Strains in the central 10-90% of a gene are color coded by the insertion's strand. Usually, "+" means that the selectable marker is encoded on the forward strand, i.e., transcribed rightward.

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500 ntAsp_trna and dnaQ are separated by 132 nucleotidesdnaQ and rnhA are separated by 55 nucleotidesrnhA and yafS overlap by 4 nucleotidesyafS and gloB are separated by 33 nucleotides OKFHMN_09960: Asp_trna - tRNA-Asp(gtc), at 1,862,635 to 1,862,711 _trna OKFHMN_09965: dnaQ - DNA polymerase III subunit epsilon, at 1,862,844 to 1,863,584 dnaQ OKFHMN_09970: rnhA - ribonuclease HI, at 1,863,640 to 1,864,107 rnhA OKFHMN_09975: yafS - Uncharacterized protein YafS, at 1,864,104 to 1,864,826 yafS OKFHMN_09980: gloB - hydroxyacylglutathione hydrolase, at 1,864,860 to 1,865,615 gloB Position (kb) 1863 1864 1865Strain fitness (log2 ratio) -2 -1 0 1 2at 1862.841 kb on + strandat 1862.846 kb on - strandat 1863.645 kb on + strandat 1863.645 kb on + strandat 1863.815 kb on + strand, within rnhAat 1863.870 kb on + strand, within rnhAat 1864.083 kb on + strandat 1864.118 kb on + strandat 1864.223 kb on + strand, within yafSat 1864.230 kb on + strand, within yafSat 1864.231 kb on - strand, within yafSat 1864.231 kb on - strand, within yafSat 1864.251 kb on - strand, within yafSat 1864.314 kb on + strand, within yafSat 1864.315 kb on - strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.532 kb on + strand, within yafSat 1864.533 kb on - strand, within yafSat 1864.533 kb on - strand, within yafSat 1864.706 kb on - strand, within yafSat 1864.854 kb on + strandat 1864.854 kb on + strandat 1864.855 kb on - strandat 1864.860 kb on - strandat 1864.860 kb on - strandat 1864.875 kb on - strandat 1864.875 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.897 kb on - strandat 1864.964 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.006 kb on - strand, within gloBat 1865.082 kb on + strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.083 kb on - strand, within gloBat 1865.106 kb on - strand, within gloB

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Per-strain Table

Position Strand Gene LocusTag Fraction Bas58
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1,862,841 + -0.3
1,862,846 - +0.3
1,863,645 + -0.5
1,863,645 + -0.2
1,863,815 + rnhA OKFHMN_09970 0.37 -0.0
1,863,870 + rnhA OKFHMN_09970 0.49 -0.4
1,864,083 + -0.3
1,864,118 + -0.4
1,864,223 + yafS OKFHMN_09975 0.16 -0.1
1,864,230 + yafS OKFHMN_09975 0.17 -2.0
1,864,231 - yafS OKFHMN_09975 0.18 +0.5
1,864,231 - yafS OKFHMN_09975 0.18 +2.7
1,864,251 - yafS OKFHMN_09975 0.20 -2.2
1,864,314 + yafS OKFHMN_09975 0.29 -0.5
1,864,315 - yafS OKFHMN_09975 0.29 -0.2
1,864,532 + yafS OKFHMN_09975 0.59 +0.1
1,864,532 + yafS OKFHMN_09975 0.59 -0.1
1,864,532 + yafS OKFHMN_09975 0.59 +0.2
1,864,532 + yafS OKFHMN_09975 0.59 -0.7
1,864,533 - yafS OKFHMN_09975 0.59 +0.1
1,864,533 - yafS OKFHMN_09975 0.59 +0.6
1,864,706 - yafS OKFHMN_09975 0.83 +0.5
1,864,854 + +0.1
1,864,854 + -0.0
1,864,855 - -1.3
1,864,860 - -1.1
1,864,860 - -0.3
1,864,875 - -0.2
1,864,875 - -0.0
1,864,897 - -1.5
1,864,897 - +2.5
1,864,897 - -0.5
1,864,897 - +0.5
1,864,897 - -1.3
1,864,964 - gloB OKFHMN_09980 0.14 -0.4
1,865,006 - gloB OKFHMN_09980 0.19 -0.6
1,865,006 - gloB OKFHMN_09980 0.19 -1.3
1,865,006 - gloB OKFHMN_09980 0.19 +0.1
1,865,006 - gloB OKFHMN_09980 0.19 -1.1
1,865,082 + gloB OKFHMN_09980 0.29 -0.3
1,865,083 - gloB OKFHMN_09980 0.29 +1.4
1,865,083 - gloB OKFHMN_09980 0.29 +0.5
1,865,083 - gloB OKFHMN_09980 0.29 -0.7
1,865,083 - gloB OKFHMN_09980 0.29 -0.3
1,865,106 - gloB OKFHMN_09980 0.33 +1.4

Or see this region's nucleotide sequence